FastQCFastQC Report
Fri 10 Feb 2017
ERR1630441.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630441.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26849
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC1780.6629669633878357No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT980.3650042832135275No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG560.2085738761220157No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT550.20484934261983684No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG530.19740027561547915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT480.1787776081045849No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG470.17505307460240607No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG470.17505307460240607No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC450.16760400759804833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT400.14898134008715408No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG390.14525680658497522No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG390.14525680658497522No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT380.14153227308279637No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG360.13408320607843868No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT360.13408320607843868No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT350.13035867257625983No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT340.12663413907408097No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC340.12663413907408097No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA340.12663413907408097No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG340.12663413907408097No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT330.12290960557190213No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG310.11546053856754443No Hit
CCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCT300.11173600506536557No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT300.11173600506536557No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG290.10801147156318672No Hit
GGACAGTGATCTGCTTGATGGCCTCTTCTGATGCAGCCTGTCC280.10428693806100785No Hit
CGCATCCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGAT270.10056240455882899No Hit
GCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGG270.10056240455882899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCCG251.2017511E-437.018
CCCGCAC251.2017511E-437.021
CCCCGCA251.2017511E-437.020
AGAAGAA401.5243095E-632.3755
TAGAAGA303.5073407E-430.8333324
CCTCGTT303.5073407E-430.83333213
CGCACAC303.5073407E-430.83333223
GCACACT303.5073407E-430.83333224
AGCCTCG358.644479E-426.4285711
GTTCCCC358.644479E-426.4285717
ACACTAG358.644479E-426.4285726
TCGTTCC358.644479E-426.4285715
AAGCCTC358.644479E-426.4285710
CTCGTTC358.644479E-426.4285714
TCCCCGC358.644479E-426.4285719
CGTTCCC400.00188267423.12516
CACACTA400.00188267423.12525
GCCTCGT400.00188267423.12512
AGAAGCC400.00188267423.1258
GAAGCCT400.00188267423.1259
Produced by FastQC (version 0.11.5)