##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630441.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26849
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62520019367574	31.0	31.0	34.0	30.0	34.0
2	31.802674215054566	31.0	31.0	34.0	30.0	34.0
3	31.92167306044918	33.0	31.0	34.0	30.0	34.0
4	35.60799284889568	37.0	35.0	37.0	33.0	37.0
5	35.54344668330292	37.0	35.0	37.0	33.0	37.0
6	35.61074900368729	37.0	35.0	37.0	33.0	37.0
7	35.59987336586092	37.0	35.0	37.0	33.0	37.0
8	35.57480725539126	37.0	35.0	37.0	33.0	37.0
9	37.267942940146746	39.0	37.0	39.0	34.0	39.0
10	37.166337666207305	39.0	37.0	39.0	33.0	39.0
11	37.24794219524004	39.0	37.0	39.0	34.0	39.0
12	37.08179075570785	39.0	37.0	39.0	33.0	39.0
13	37.12480911765801	39.0	37.0	39.0	33.0	39.0
14	38.281016052739396	40.0	38.0	41.0	33.0	41.0
15	38.28053186338411	40.0	38.0	41.0	33.0	41.0
16	38.23066035978994	40.0	38.0	41.0	33.0	41.0
17	38.23509255465753	40.0	38.0	41.0	33.0	41.0
18	38.09572051100599	40.0	37.0	41.0	33.0	41.0
19	38.0849566091847	40.0	37.0	41.0	33.0	41.0
20	38.023613542403815	40.0	37.0	41.0	33.0	41.0
21	38.015121606018845	40.0	37.0	41.0	33.0	41.0
22	37.908935155871724	40.0	37.0	41.0	33.0	41.0
23	37.87217401020522	40.0	37.0	41.0	33.0	41.0
24	37.84610227568997	40.0	37.0	41.0	33.0	41.0
25	37.76781258147417	40.0	37.0	41.0	32.0	41.0
26	37.5596484040374	40.0	37.0	41.0	32.0	41.0
27	37.468062125218815	39.0	37.0	41.0	32.0	41.0
28	37.313568475548436	39.0	36.0	41.0	31.0	41.0
29	37.241238034936124	39.0	36.0	41.0	31.0	41.0
30	37.179150061454806	39.0	36.0	41.0	31.0	41.0
31	37.08696785727587	39.0	36.0	41.0	31.0	41.0
32	36.982159484524566	39.0	36.0	41.0	31.0	41.0
33	36.874371484971505	39.0	36.0	41.0	30.0	41.0
34	36.7234161421282	39.0	35.0	40.0	30.0	41.0
35	36.604826995418826	39.0	35.0	40.0	30.0	41.0
36	36.46299675965585	39.0	35.0	40.0	30.0	41.0
37	36.42139372043651	39.0	35.0	40.0	30.0	41.0
38	36.26198368654326	39.0	35.0	40.0	29.0	41.0
39	36.16715706357779	39.0	35.0	40.0	29.0	41.0
40	36.05501135982718	39.0	35.0	40.0	28.0	41.0
41	35.86930611940854	38.0	35.0	40.0	28.0	41.0
42	35.734775969309844	38.0	34.0	40.0	27.0	41.0
43	34.45137621512905	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	10.0
21	17.0
22	25.0
23	44.0
24	66.0
25	110.0
26	157.0
27	244.0
28	319.0
29	446.0
30	531.0
31	737.0
32	860.0
33	1126.0
34	1526.0
35	1959.0
36	2502.0
37	3618.0
38	6171.0
39	6375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.599389176505646	16.663562888748185	11.784424000893887	27.952623933852283
2	22.190770605981598	18.808894186003204	31.22276434876532	27.777570859249877
3	21.51663004208723	20.224216916831168	28.377220753100673	29.881932287980927
4	16.734329025289583	14.629967596558533	30.24321203769228	38.39249134045961
5	17.184997579053224	34.787142910350475	31.528176095943984	16.499683414652313
6	39.167194308912805	30.82796379753436	13.639241684978956	16.365600208573877
7	31.613840366494095	27.956348467354463	19.062162464151363	21.367648702000075
8	29.75529814890685	31.1371000782152	18.19434615814369	20.913255614734254
9	27.974971134865356	12.804946180490894	16.913106633394168	42.30697605124958
10	19.941152370665574	25.010242467130993	28.90982904391225	26.138776118291183
11	38.966069499795154	19.356400610823492	18.220417892658944	23.45711199672241
12	25.14805020671161	22.701031695780102	25.956273976684418	26.194644120823867
13	33.17441990390704	16.726879958285224	22.6488882267496	27.44981191105814
14	24.481358709821595	18.961600059592538	22.44776341763194	34.10927781295393
15	29.945249357517973	23.68058400685314	19.103132332675333	27.271034302953556
16	27.08853216134679	23.00271890945659	20.53335319751201	29.375395731684605
17	25.583820626466537	24.652687250921822	22.52225408767552	27.24123803493612
18	26.73097694513762	21.38627136951097	23.74390107639018	28.13885060896123
19	27.852061529293454	22.302506611046965	22.49245781965809	27.352974040001488
20	28.261760214533126	22.175872471972884	22.727103430295355	26.83526388319863
21	30.41081604529033	21.136727624864985	21.676784982680918	26.775671347163765
22	29.226414391597455	22.198219672985957	21.93005326082908	26.645312674587508
23	28.13512607545905	22.697307162277923	21.54270177660248	27.624864985660547
24	28.086707139930724	22.49245781965809	22.496182353160265	26.924652687250923
25	27.825989794778206	22.28760847703825	22.764348765317145	27.122052962866398
26	29.613765875824054	21.83693992327461	22.25408767551864	26.295206525382696
27	29.68825654586763	21.583671645126447	21.252188163432532	27.47588364557339
28	26.827814816194273	21.28943349845432	23.51670453275727	28.36604715259414
29	26.757048679652872	21.96729859585087	24.153599761629856	27.122052962866398
30	26.60806733956572	23.1070058475176	23.46456106372677	26.82036574918991
31	27.110879362359864	22.730827963797534	22.987820775447876	27.170471898394727
32	26.82036574918991	21.795970054750644	23.24108905359604	28.142575142463404
33	27.539200715110436	22.742001564304072	22.723378896793175	26.99541882379232
34	27.58389511713658	22.633990092740884	22.645163693247422	27.136951096875116
35	28.187269544489553	21.54642631010466	23.75135014339454	26.514954002011248
36	26.541025736526503	23.2299154530895	21.851838057283327	28.377220753100673
37	26.01586651271928	22.58184662371038	23.572572535289954	27.82971432828038
38	27.52430258110172	21.9263287273269	23.49063279824202	27.05873589332936
39	25.725352899549332	22.350925546575294	24.39569443927148	27.528027114603898
40	26.354799061417555	20.689783604603523	25.986070244701853	26.96934708927707
41	24.36962270475623	21.781071920741926	26.056836381243247	27.792468993258595
42	24.783045923498083	22.15724980446199	25.360348616335806	27.69935565570412
43	24.40686803977802	19.87783530112853	26.798018548176838	28.917278110916605
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	4.0
4	5.0
5	5.0
6	11.5
7	18.0
8	16.5
9	15.0
10	15.5
11	16.0
12	16.0
13	28.0
14	40.0
15	74.0
16	108.0
17	86.0
18	64.0
19	64.0
20	77.5
21	91.0
22	82.5
23	74.0
24	81.0
25	88.0
26	88.0
27	110.5
28	133.0
29	166.0
30	199.0
31	255.5
32	312.0
33	312.0
34	376.0
35	440.0
36	522.5
37	605.0
38	718.5
39	832.0
40	832.0
41	914.0
42	996.0
43	1103.5
44	1211.0
45	1372.0
46	1533.0
47	1533.0
48	1645.0
49	1757.0
50	1803.5
51	1850.0
52	2105.0
53	2360.0
54	2360.0
55	2404.5
56	2449.0
57	2319.0
58	2189.0
59	2090.5
60	1992.0
61	1992.0
62	1898.0
63	1804.0
64	1610.5
65	1417.0
66	1210.5
67	1004.0
68	1004.0
69	899.5
70	795.0
71	722.5
72	650.0
73	675.0
74	700.0
75	700.0
76	648.0
77	596.0
78	468.0
79	340.0
80	219.5
81	99.0
82	99.0
83	64.0
84	29.0
85	24.5
86	20.0
87	16.5
88	13.0
89	13.0
90	7.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	26849.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.25974896644195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.4353495837095	67.15706357778689
2	4.7998796268432145	7.128757123170323
3	1.6701775504062593	3.7208089686766734
4	0.8375965493028388	2.4879883794554734
5	0.5466947537365834	2.0298707586874745
6	0.38619721135520113	1.7207344780066296
7	0.1554819941819641	0.808223769972811
8	0.1655130905808005	0.983276844575217
9	0.16049754238138228	1.0726656486275095
>10	0.817534356505166	11.251815710082312
>50	0.020062192797672785	0.9758277775708593
>100	0.005015548199418196	0.6629669633878357
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	178	0.6629669633878357	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	98	0.3650042832135275	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	56	0.2085738761220157	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	55	0.20484934261983684	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	53	0.19740027561547915	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	48	0.1787776081045849	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	47	0.17505307460240607	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	47	0.17505307460240607	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	45	0.16760400759804833	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40	0.14898134008715408	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	39	0.14525680658497522	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	39	0.14525680658497522	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	38	0.14153227308279637	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	36	0.13408320607843868	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	36	0.13408320607843868	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	35	0.13035867257625983	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	34	0.12663413907408097	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	34	0.12663413907408097	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	34	0.12663413907408097	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	34	0.12663413907408097	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	33	0.12290960557190213	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	31	0.11546053856754443	No Hit
CCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCT	30	0.11173600506536557	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	30	0.11173600506536557	No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG	29	0.10801147156318672	No Hit
GGACAGTGATCTGCTTGATGGCCTCTTCTGATGCAGCCTGTCC	28	0.10428693806100785	No Hit
CGCATCCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGAT	27	0.10056240455882899	No Hit
GCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGG	27	0.10056240455882899	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.011173600506536556	0.0
18	0.0	0.0	0.0	0.011173600506536556	0.0
19	0.0	0.0	0.0	0.011173600506536556	0.0
20	0.0	0.0	0.0	0.011173600506536556	0.0
21	0.0	0.0	0.0	0.014898134008715409	0.0
22	0.0	0.0	0.0	0.026071734515251965	0.0
23	0.0	0.0	0.0	0.03352080151960967	0.0
24	0.0	0.0	0.0	0.05214346903050393	0.0
25	0.0	0.0	0.0	0.05586800253268278	0.0
26	0.0	0.0	0.0	0.06331706953704049	0.0
27	0.0	0.0	0.0	0.0782152035457559	0.0
28	0.0	0.0	0.0	0.13035867257625983	0.0
29	0.0	0.0	0.0	0.19740027561547915	0.0
30	0.0	0.0	0.0	0.2793400126634139	0.0
31	0.0	0.0	0.0	0.4692912212745354	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCCCCG	25	1.2017511E-4	37.0	18
CCCGCAC	25	1.2017511E-4	37.0	21
CCCCGCA	25	1.2017511E-4	37.0	20
AGAAGAA	40	1.5243095E-6	32.375	5
TAGAAGA	30	3.5073407E-4	30.833332	4
CCTCGTT	30	3.5073407E-4	30.833332	13
CGCACAC	30	3.5073407E-4	30.833332	23
GCACACT	30	3.5073407E-4	30.833332	24
AGCCTCG	35	8.644479E-4	26.42857	11
GTTCCCC	35	8.644479E-4	26.42857	17
ACACTAG	35	8.644479E-4	26.42857	26
TCGTTCC	35	8.644479E-4	26.42857	15
AAGCCTC	35	8.644479E-4	26.42857	10
CTCGTTC	35	8.644479E-4	26.42857	14
TCCCCGC	35	8.644479E-4	26.42857	19
CGTTCCC	40	0.001882674	23.125	16
CACACTA	40	0.001882674	23.125	25
GCCTCGT	40	0.001882674	23.125	12
AGAAGCC	40	0.001882674	23.125	8
GAAGCCT	40	0.001882674	23.125	9
>>END_MODULE