FastQCFastQC Report
Fri 10 Feb 2017
ERR1630440.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630440.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158464
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42962.7110258481421647No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33232.0970062600969306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23461.480462439418417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13290.8386762924071083No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8100.5111571082390953No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG7980.5035844103392569No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA5960.376110662358643No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5020.3167911954765751No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.3104806138933764No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4080.25747172859450723No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4070.2568406704361874No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4020.25368537964458804No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3230.20383178513731826No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3140.1981522617124394No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.1981522617124394No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2940.185531098546042No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2870.1811136914378029No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2800.1766962843295638No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2790.17606522617124393No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.1722788772213247No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC2680.16912358642972536No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG2430.1533471324717286No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.14577443457189013No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2240.14135702746365103No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA2190.13820173667205168No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA2180.1375706785137318No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2030.12810480613893377No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1930.12179422455573506No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1910.12053210823909531No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA1860.11737681744749597No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.11674575928917609No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG1760.11106623586429726No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA1750.11043517770597738No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1680.10601777059773829No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.10601777059773829No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC1680.10601777059773829No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1670.10538671243941841No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1660.10475565428109855No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1620.10223142164781907No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.10096930533117932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG251.2288278E-437.01
GTTGAAT250.00548222929.63
GTGGGGA250.00548222929.611
GTCGTCA250.00548222929.624
CATACAG250.00548222929.628
TACTAGG250.00548222929.62
GCAATTA250.00548222929.631
GCATACA250.00548222929.627
TACTGTG358.835548E-426.4285727
TCTTATA751.3547833E-824.66666637
CCAATGC400.001923887923.12518
TCCAATG400.001923887923.12517
CAATGCG400.001923887923.12519
CATCCAA400.001923887923.12515
TGTGTCA400.001923887923.12529
TGCGACG502.6878814E-422.222
GTAGTAG502.6878814E-422.23
CGCTGAA450.003811485120.55555718
GATACTG500.007008797518.534
CTTCACC500.007008797518.532
Produced by FastQC (version 0.11.5)