##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630440.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	158464
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.800680280694667	31.0	31.0	34.0	30.0	34.0
2	31.912844557754443	33.0	31.0	34.0	30.0	34.0
3	31.961738943861068	33.0	31.0	34.0	30.0	34.0
4	35.66633430936995	37.0	35.0	37.0	33.0	37.0
5	35.63632118336026	37.0	35.0	37.0	33.0	37.0
6	35.75791346930533	37.0	35.0	37.0	35.0	37.0
7	35.73453276453958	37.0	35.0	37.0	33.0	37.0
8	35.7739991417609	37.0	35.0	37.0	35.0	37.0
9	37.395888025040385	39.0	37.0	39.0	34.0	39.0
10	37.3212906401454	39.0	37.0	39.0	34.0	39.0
11	37.44161449919225	39.0	37.0	39.0	35.0	39.0
12	37.34704412358643	39.0	37.0	39.0	34.0	39.0
13	37.382427554523424	39.0	37.0	39.0	34.0	39.0
14	38.45149055936995	40.0	38.0	41.0	34.0	41.0
15	38.43582138529887	40.0	38.0	41.0	34.0	41.0
16	38.27424525444265	40.0	38.0	41.0	34.0	41.0
17	38.374880098949916	40.0	38.0	41.0	34.0	41.0
18	38.46381512520194	40.0	38.0	41.0	34.0	41.0
19	38.510071688206786	40.0	38.0	41.0	34.0	41.0
20	38.539920739095315	40.0	38.0	41.0	34.0	41.0
21	38.520073960016155	40.0	38.0	41.0	34.0	41.0
22	38.477319769789986	40.0	38.0	41.0	34.0	41.0
23	38.42577493941842	40.0	38.0	41.0	34.0	41.0
24	38.40286752827141	40.0	38.0	41.0	34.0	41.0
25	38.35594204361874	40.0	38.0	41.0	34.0	41.0
26	38.11520597738288	40.0	37.0	41.0	34.0	41.0
27	37.98885551292407	40.0	37.0	41.0	33.0	41.0
28	37.85388479402262	40.0	37.0	41.0	33.0	41.0
29	37.705327392972535	40.0	36.0	41.0	33.0	41.0
30	37.513870658319874	39.0	36.0	41.0	33.0	41.0
31	37.23031098546042	39.0	35.0	41.0	33.0	41.0
32	36.958129291195476	39.0	35.0	41.0	32.0	41.0
33	36.81761157108239	39.0	35.0	41.0	31.0	41.0
34	36.58921900242326	39.0	35.0	40.0	31.0	41.0
35	36.419672607027465	39.0	35.0	40.0	31.0	41.0
36	36.21373939822294	38.0	35.0	40.0	30.0	41.0
37	35.978234804119545	38.0	35.0	40.0	30.0	41.0
38	35.76470365508885	38.0	35.0	40.0	29.0	41.0
39	35.46659178109854	38.0	35.0	40.0	27.0	41.0
40	35.188383481421646	38.0	35.0	40.0	25.0	41.0
41	34.8376287358643	38.0	34.0	40.0	24.0	41.0
42	34.553513731825525	38.0	34.0	40.0	22.0	41.0
43	33.44565958198707	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	1.0
13	0.0
14	3.0
15	0.0
16	1.0
17	1.0
18	7.0
19	12.0
20	29.0
21	53.0
22	139.0
23	183.0
24	356.0
25	561.0
26	909.0
27	1355.0
28	1819.0
29	2455.0
30	3201.0
31	4016.0
32	5123.0
33	6502.0
34	8360.0
35	10681.0
36	15341.0
37	25310.0
38	34095.0
39	37946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.33269386106624	19.49654180129241	13.755805735056542	21.414958602584814
2	18.349909127625203	23.98778271405493	32.82638327948303	24.835924878836835
3	22.533824717285945	25.646203554119545	30.700348344103396	21.119623384491113
4	14.545890549273022	18.371996163166397	32.501388327948305	34.58072495961228
5	12.375681542810986	41.158244143780294	32.13537459612278	14.330699717285944
6	29.462212237479807	31.93785339256866	18.99548162358643	19.604452746365105
7	25.78188105815832	34.595870355411954	21.025595718901453	18.59665286752827
8	31.148399636510497	31.0436439822294	20.038620759289177	17.76933562197092
9	28.884162964458802	12.804170032310177	21.68694466882068	36.624722334410336
10	21.38719204361874	28.703049273021	28.22849353796446	21.681265145395802
11	31.299853594507272	25.04354301292407	18.781552907915994	24.875050484652668
12	21.067876615508887	30.517972536348946	28.22533824717286	20.188812600969307
13	32.507067851373186	20.092891760904685	26.46531704361874	20.934723344103393
14	22.935177705977384	23.14405795638126	28.99964660743134	24.921117730210014
15	30.66690226171244	24.059723344103393	24.85738085621971	20.415993537964457
16	21.86679624394184	25.619068053311793	30.810152463651054	21.703983239095315
17	20.290412964458802	29.118916599353796	29.49944466882068	21.09122576736672
18	20.032310177705977	23.461480210016155	33.09647617124394	23.409733441033925
19	20.495506865912763	27.945148424878834	34.08029584006462	17.479048869143778
20	21.37520193861066	23.244396203554118	35.19411348949919	20.186288368336026
21	21.941261106623585	24.402387924071082	33.904861672051695	19.751489297253634
22	22.476398424878838	24.510298869143778	31.665236268174475	21.348066437802906
23	20.292937197092083	25.345188812600973	32.66357027463651	21.698303715670438
24	19.949010500807756	26.85278675282714	33.270648222940224	19.927554523424877
25	19.841730613893375	26.165564418416803	32.196587237479804	21.796117730210014
26	20.99972233441034	26.026100565428113	32.625075726979	20.349101373182553
27	20.58448606623586	25.96930533117932	31.874747576736674	21.571461025848144
28	19.222031502423263	27.197975565428113	33.07060278675283	20.509390145395802
29	19.04722839256866	27.225742124394188	33.1684168012924	20.55861268174475
30	17.992099151857836	29.75376110662359	34.3844658723748	17.869673869143778
31	19.766634693053312	28.779407310177707	31.74979806138934	19.704159935379646
32	17.602105210016155	29.366291397415186	33.64297253634895	19.38863085621971
33	19.637898828756057	28.505528069466884	32.78536449919224	19.071208602584814
34	18.47801393376414	28.956103594507272	31.84319466882068	20.722687802907917
35	18.633885298869142	30.14059975767367	32.23634390145396	18.989171042003232
36	19.374747576736674	29.69444163974152	31.36863893376414	19.56217184975767
37	18.680583602584814	30.274384087237483	31.54028675282714	19.504745557350567
38	19.504114499192244	29.49502726171244	32.18901453957997	18.811843699515347
39	19.748965064620354	28.211454967689818	32.19153877221325	19.848041195476576
40	19.106547859450725	29.49628937802908	31.3484450726979	20.048717689822293
41	18.047632269789986	29.322748384491113	31.232330371567045	21.397288974151856
42	17.81287863489499	29.604200323101775	31.641887116316642	20.941033925686593
43	18.01986571082391	29.378912560581583	31.44562802907916	21.155593699515347
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	43.0
2	65.0
3	229.5
4	394.0
5	394.0
6	574.0
7	754.0
8	733.0
9	712.0
10	911.5
11	1111.0
12	1111.0
13	2240.0
14	3369.0
15	5006.5
16	6644.0
17	5791.5
18	4939.0
19	4939.0
20	6017.5
21	7096.0
22	4978.0
23	2860.0
24	2831.0
25	2802.0
26	2802.0
27	2946.0
28	3090.0
29	3013.5
30	2937.0
31	3121.5
32	3306.0
33	3306.0
34	3614.5
35	3923.0
36	4276.0
37	4629.0
38	5114.0
39	5599.0
40	5599.0
41	6077.0
42	6555.0
43	7408.5
44	8262.0
45	9439.5
46	10617.0
47	10617.0
48	14893.0
49	19169.0
50	18752.0
51	18335.0
52	14272.0
53	10209.0
54	10209.0
55	9066.0
56	7923.0
57	7547.0
58	7171.0
59	6338.0
60	5505.0
61	5505.0
62	4571.0
63	3637.0
64	3113.0
65	2589.0
66	2123.5
67	1658.0
68	1658.0
69	1381.0
70	1104.0
71	867.0
72	630.0
73	520.0
74	410.0
75	410.0
76	332.5
77	255.0
78	177.0
79	99.0
80	76.5
81	54.0
82	54.0
83	37.0
84	20.0
85	13.0
86	6.0
87	4.0
88	2.0
89	2.0
90	2.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	158464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.8657613085622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.46539222148978	37.55805735056543
2	11.117996044825313	10.643426898222941
3	3.72972972972973	5.355790589660743
4	1.8075148319050758	3.460722940226171
5	1.074489123269611	2.5715619951534734
6	0.7369808833223467	2.1165690630048464
7	0.5194462755438365	1.7404584006462036
8	0.4073829927488464	1.5599757673667205
9	0.2966381015161503	1.2778927705977383
>10	1.6427158866183258	13.92997778675283
>50	0.10810810810810811	3.503003836833603
>100	0.08305866842452209	7.447748384491115
>500	0.005273566249176006	1.7076433764135701
>1k	0.005273566249176006	7.12717084006462
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4296	2.7110258481421647	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3323	2.0970062600969306	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2346	1.480462439418417	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1329	0.8386762924071083	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	810	0.5111571082390953	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	798	0.5035844103392569	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	596	0.376110662358643	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	502	0.3167911954765751	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	492	0.3104806138933764	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	408	0.25747172859450723	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	407	0.2568406704361874	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	402	0.25368537964458804	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	323	0.20383178513731826	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	314	0.1981522617124394	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	314	0.1981522617124394	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	294	0.185531098546042	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	287	0.1811136914378029	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	280	0.1766962843295638	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	279	0.17606522617124393	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	273	0.1722788772213247	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	268	0.16912358642972536	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	243	0.1533471324717286	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	231	0.14577443457189013	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	224	0.14135702746365103	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	219	0.13820173667205168	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	218	0.1375706785137318	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	203	0.12810480613893377	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	193	0.12179422455573506	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	191	0.12053210823909531	No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA	186	0.11737681744749597	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	185	0.11674575928917609	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	176	0.11106623586429726	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	175	0.11043517770597738	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	168	0.10601777059773829	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	168	0.10601777059773829	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	168	0.10601777059773829	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	167	0.10538671243941841	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	166	0.10475565428109855	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	162	0.10223142164781907	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	160	0.10096930533117932	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0012621163166397415	0.0
14	0.0	0.0	0.0	0.0012621163166397415	0.0
15	0.0	0.0	0.0	0.0012621163166397415	0.0
16	0.0	0.0	0.0	0.0031552907915993536	0.0
17	0.0	0.0	0.0	0.005679523424878837	0.0
18	0.0	0.0	0.0	0.006941639741518579	0.0
19	0.0	0.0	0.0	0.00820375605815832	0.0
20	0.0	0.0	0.0	0.008834814216478191	0.0
21	0.0	0.0	0.0	0.010727988691437804	0.0
22	0.0	0.0	0.0	0.017669628432956382	0.0
23	0.0	0.0	0.0	0.023349151857835218	0.0
24	0.0	0.0	0.0	0.044805129240710825	0.0
25	0.0	0.0	0.0	0.04859147819063005	0.0
26	0.0	0.0	0.0	0.059950525040387725	0.0
27	0.0	0.0	0.0	0.06878533925686592	0.0
28	0.0	0.0	0.0	0.09024131663974151	0.0
29	0.0	0.0	0.0	0.12684268982229402	0.0
30	0.0	0.0	0.0	0.1836379240710824	0.0
31	0.0	0.0	0.0	0.3262570678513732	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	25	1.2288278E-4	37.0	1
GTTGAAT	25	0.005482229	29.6	3
GTGGGGA	25	0.005482229	29.6	11
GTCGTCA	25	0.005482229	29.6	24
CATACAG	25	0.005482229	29.6	28
TACTAGG	25	0.005482229	29.6	2
GCAATTA	25	0.005482229	29.6	31
GCATACA	25	0.005482229	29.6	27
TACTGTG	35	8.835548E-4	26.428572	7
TCTTATA	75	1.3547833E-8	24.666666	37
CCAATGC	40	0.0019238879	23.125	18
TCCAATG	40	0.0019238879	23.125	17
CAATGCG	40	0.0019238879	23.125	19
CATCCAA	40	0.0019238879	23.125	15
TGTGTCA	40	0.0019238879	23.125	29
TGCGACG	50	2.6878814E-4	22.2	22
GTAGTAG	50	2.6878814E-4	22.2	3
CGCTGAA	45	0.0038114851	20.555557	18
GATACTG	50	0.0070087975	18.5	34
CTTCACC	50	0.0070087975	18.5	32
>>END_MODULE