FastQCFastQC Report
Fri 10 Feb 2017
ERR1630439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839520
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA200.001841653537.015
AACGAAC502.7012613E-422.214
TCAATCG450.00382496120.55555734
AGTCTAG450.00382496120.55555719
TAACGAA555.1415054E-420.18181813
GACGGAT555.1415054E-420.1818187
CCTATAT555.1415054E-420.1818183
GGTATCA3350.019.8805961
TAACGCC759.260895E-619.7333324
CCCCTAT701.2187003E-418.51
CGAACGA609.23399E-418.516
GTATAGC500.007033340618.53
GTATTAG1653.6379788E-1217.9393941
ATAACGC904.4436583E-516.4444453
TCGTTTA803.3812068E-416.187530
AATTCCG803.3812068E-416.18755
TCTAGAC700.002591712615.8571433
ACGGATC700.002591712615.8571438
TATAGCT700.002591712615.8571434
GGGTTAG700.002591712615.8571431