##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630439.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	839520
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.800526491328377	31.0	31.0	34.0	30.0	34.0
2	31.992002572898798	33.0	31.0	34.0	30.0	34.0
3	32.151774823708784	34.0	31.0	34.0	30.0	34.0
4	35.80889794168096	37.0	35.0	37.0	35.0	37.0
5	35.72598866018677	37.0	35.0	37.0	35.0	37.0
6	35.78576924909472	37.0	35.0	37.0	35.0	37.0
7	35.75183795502192	37.0	35.0	37.0	33.0	37.0
8	35.71850104821803	37.0	35.0	37.0	33.0	37.0
9	37.446727892128834	39.0	37.0	39.0	35.0	39.0
10	37.34493162759672	39.0	37.0	39.0	34.0	39.0
11	37.433030779493045	39.0	37.0	39.0	34.0	39.0
12	37.291527301315035	39.0	37.0	39.0	34.0	39.0
13	37.33244711263579	39.0	37.0	39.0	34.0	39.0
14	38.54997736801982	40.0	38.0	41.0	34.0	41.0
15	38.538674480655615	40.0	38.0	41.0	34.0	41.0
16	38.52204235753764	40.0	38.0	41.0	34.0	41.0
17	38.47582666285496	40.0	38.0	41.0	34.0	41.0
18	38.4407220792834	40.0	38.0	41.0	34.0	41.0
19	38.44058390508862	40.0	38.0	41.0	34.0	41.0
20	38.38599676005337	40.0	38.0	41.0	34.0	41.0
21	38.3473234705546	40.0	38.0	41.0	34.0	41.0
22	38.320393796455114	40.0	38.0	41.0	34.0	41.0
23	38.25817848294263	40.0	38.0	41.0	34.0	41.0
24	38.23960239184296	40.0	38.0	41.0	34.0	41.0
25	38.199356775300174	40.0	38.0	41.0	33.0	41.0
26	38.05051577091671	40.0	38.0	41.0	33.0	41.0
27	37.950484800838574	40.0	37.0	41.0	33.0	41.0
28	37.85786878216123	40.0	37.0	41.0	33.0	41.0
29	37.79193944158567	40.0	37.0	41.0	33.0	41.0
30	37.7358728797408	40.0	37.0	41.0	32.0	41.0
31	37.64572493805984	40.0	37.0	41.0	32.0	41.0
32	37.54554626453211	40.0	37.0	41.0	32.0	41.0
33	37.48203259005146	40.0	37.0	41.0	32.0	41.0
34	37.381029635982465	39.0	37.0	41.0	31.0	41.0
35	37.3007373260911	39.0	36.0	41.0	31.0	41.0
36	37.20012507146941	39.0	36.0	41.0	31.0	41.0
37	37.14085787116448	39.0	36.0	41.0	31.0	41.0
38	37.0456868210406	39.0	36.0	41.0	31.0	41.0
39	36.95135077186964	39.0	36.0	41.0	30.0	41.0
40	36.85161639984753	39.0	35.0	41.0	30.0	41.0
41	36.734790118162756	39.0	35.0	40.0	30.0	41.0
42	36.68753097007814	39.0	35.0	40.0	30.0	41.0
43	35.411743615399274	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	5.0
17	15.0
18	36.0
19	73.0
20	113.0
21	260.0
22	482.0
23	876.0
24	1468.0
25	2236.0
26	3361.0
27	5057.0
28	7053.0
29	9727.0
30	13469.0
31	17997.0
32	22995.0
33	29869.0
34	40158.0
35	53923.0
36	75860.0
37	111762.0
38	200713.0
39	242008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.19515913855537	17.878192300362112	11.49752239374881	27.429126167333717
2	19.614065180102916	19.754026110158186	32.22698684962836	28.404921860110537
3	20.043477225081	21.356846769582617	28.95392605298266	29.64574995235373
4	15.138650657518582	14.922455688965123	33.44077568134172	36.498117972174576
5	15.848341909662663	35.00393081761006	33.69473032208881	15.45299695063846
6	37.293929864684586	33.79395368782161	14.616804840861445	14.29531160663236
7	31.472269868496284	28.89913283781208	20.125547932151704	19.50304936153993
8	29.161425576519918	32.011983037926434	18.79038021726701	20.03621116828664
9	27.396488469601678	13.193729750333524	17.899871355060036	41.50991042500476
10	17.469625500285876	25.34507813988946	32.393391461787694	24.79190489803697
11	38.998117972174576	20.00476462740614	19.880765199161427	21.11635220125786
12	23.137030684200496	23.44494473032209	28.10308271393177	25.314941871545642
13	32.35277301315037	18.054602630074328	22.878192300362112	26.71443205641319
14	23.848985134362493	18.662926434152848	23.127977892128833	34.36011053935582
15	26.87011625690871	25.937797789212887	20.36735277301315	26.824733180865255
16	27.03783114160473	24.513293310463123	22.003764055650848	26.445111492281303
17	25.736968744044216	24.454569277682488	23.52094053744997	26.287521440823326
18	26.079902801600912	23.551315037164095	24.33188012197446	26.03690203926053
19	26.548623022679624	23.660305889079474	23.78799790356394	26.003073184676957
20	26.774228130360207	23.313083666857253	23.760005717552886	26.152682485229654
21	27.141461787688204	23.52618162759672	23.256503716409377	26.0758528683057
22	27.051410329712215	23.305579378692588	23.41504669334858	26.227963598246617
23	26.39412997903564	23.19956641890604	23.642557651991613	26.763745950066703
24	26.388650657518582	23.745354488279016	23.425767105012387	26.440227749190015
25	26.86320754716981	23.24209071850581	23.492591004383456	26.40211072994092
26	26.635339241471318	23.665904326281684	23.43934629311988	26.25941013912712
27	26.634624547360396	23.32666285496474	23.445540308747855	26.593172288927008
28	26.43343815513627	23.406470364017533	23.73117972174576	26.428911759100437
29	25.968410520297315	23.76524680769964	24.06613302839718	26.20020964360587
30	26.178887935963406	24.066252144082334	23.57835429769392	26.176505622260336
31	26.7233657327997	23.871736230226794	23.21862492853059	26.18627310844292
32	25.994139508290452	23.847913093196112	23.40944825614637	26.748499142367066
33	25.92552887364208	23.523561082523347	23.978702115494567	26.572207928340003
34	26.484181437011628	23.235301124452068	23.82766342672003	26.452854011816274
35	26.211406518010293	23.727010672765388	24.17691061558986	25.884672193634454
36	25.94256241661902	24.098651610444062	23.705927196493235	26.252858776443684
37	26.184724604535926	23.449352010672765	24.078878406708597	26.28704497808271
38	25.761983037926434	23.773465789975223	24.47184105202973	25.99271012006861
39	25.7833047455689	23.37847817800648	24.530088622069755	26.30812845435487
40	25.62607204116638	23.310344006098724	24.965694682675814	26.09788927005908
41	25.184033733562032	23.143939393939394	25.3219696969697	26.350057175528875
42	25.284686487516677	23.185510768057938	25.475867162187914	26.053935582237468
43	25.049671240708975	22.215789975223938	25.80510291595197	26.929435868115114
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	6.0
2	9.0
3	24.0
4	39.0
5	39.0
6	61.0
7	83.0
8	82.0
9	81.0
10	106.0
11	131.0
12	131.0
13	245.5
14	360.0
15	601.0
16	842.0
17	932.5
18	1023.0
19	1023.0
20	1340.5
21	1658.0
22	1929.0
23	2200.0
24	3095.0
25	3990.0
26	3990.0
27	5081.5
28	6173.0
29	7918.5
30	9664.0
31	11985.5
32	14307.0
33	14307.0
34	17229.5
35	20152.0
36	23980.0
37	27808.0
38	31485.0
39	35162.0
40	35162.0
41	39042.0
42	42922.0
43	45863.5
44	48805.0
45	52265.0
46	55725.0
47	55725.0
48	58482.0
49	61239.0
50	63067.0
51	64895.0
52	66021.0
53	67147.0
54	67147.0
55	67407.5
56	67668.0
57	65926.5
58	64185.0
59	62312.0
60	60439.0
61	60439.0
62	56267.0
63	52095.0
64	46860.5
65	41626.0
66	36511.0
67	31396.0
68	31396.0
69	26792.0
70	22188.0
71	18687.0
72	15186.0
73	11984.0
74	8782.0
75	8782.0
76	6920.5
77	5059.0
78	3995.0
79	2931.0
80	2395.0
81	1859.0
82	1859.0
83	1344.0
84	829.0
85	633.5
86	438.0
87	334.5
88	231.0
89	231.0
90	194.5
91	158.0
92	88.0
93	18.0
94	14.0
95	10.0
96	10.0
97	6.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	839520.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.15078342285689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.2797162213979	58.97159186788582
2	8.908134368914746	12.320089408930631
3	2.4826568829715914	5.150330052829005
4	1.0440457081525043	2.887863145920683
5	0.5679403412736973	1.9636759768260434
6	0.3732868986381379	1.5487848889389486
7	0.2450764615585536	1.1863060520692956
8	0.1817542578281421	1.0054759455404758
9	0.1308414843673785	0.8143012033392336
>10	0.7169462530386578	9.066964369041695
>50	0.04715078545362579	2.2493504828243527
>100	0.022277641512859138	2.7703021960876195
>500	1.726948922088899E-4	0.06496440976617586
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	2.38231370306842E-4	0.0
18	0.0	0.0	0.0	3.57347055460263E-4	0.0
19	0.0	0.0	0.0	3.57347055460263E-4	0.0
20	0.0	0.0	0.0	5.95578425767105E-4	0.0
21	0.0	0.0	0.0	0.001072041166380789	0.0
22	0.0	0.0	0.0	0.001429388221841052	0.0
23	0.0	0.0	0.0	0.001905850962454736	0.0
24	0.0	0.0	0.0	0.0036925862397560513	0.0
25	0.0	0.0	0.0	0.004049933295216314	0.0
26	0.0	0.0	0.0	0.004883743091290261	0.0
27	0.0	0.0	0.0	0.009052792071659996	0.0
28	0.0	0.0	0.0	0.021559939012769203	0.0
29	0.0	0.0	0.0	0.04014198589670288	0.0
30	0.0	0.0	0.0	0.06551362683438156	0.0
31	0.0	0.0	0.0	0.12411854392986468	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	20	0.0018416535	37.0	15
AACGAAC	50	2.7012613E-4	22.2	14
TCAATCG	45	0.003824961	20.555557	34
AGTCTAG	45	0.003824961	20.555557	19
TAACGAA	55	5.1415054E-4	20.181818	13
GACGGAT	55	5.1415054E-4	20.181818	7
CCTATAT	55	5.1415054E-4	20.181818	3
GGTATCA	335	0.0	19.880596	1
TAACGCC	75	9.260895E-6	19.733332	4
CCCCTAT	70	1.2187003E-4	18.5	1
CGAACGA	60	9.23399E-4	18.5	16
GTATAGC	50	0.0070333406	18.5	3
GTATTAG	165	3.6379788E-12	17.939394	1
ATAACGC	90	4.4436583E-5	16.444445	3
TCGTTTA	80	3.3812068E-4	16.1875	30
AATTCCG	80	3.3812068E-4	16.1875	5
TCTAGAC	70	0.0025917126	15.857143	3
ACGGATC	70	0.0025917126	15.857143	8
TATAGCT	70	0.0025917126	15.857143	4
GGGTTAG	70	0.0025917126	15.857143	1
>>END_MODULE