FastQCFastQC Report
Fri 10 Feb 2017
ERR1630438.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630438.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759606
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36490.4803806183732093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35480.46708425157252575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25780.3393864714075455No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG21540.28356806028388404No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16620.21879763982906927No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG14460.19036184548305307No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT10480.13796626145659724No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT10120.13322696239892787No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA8600.11321658859987942No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC8460.11137352785523022No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7640.10057845777942775No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC250.005495184629.636
TCTGTCG601.3364097E-624.6666688
GCGTTAT751.3722456E-824.6666661
CGTTATT652.6808575E-622.769232
CTAGCGG502.7009333E-422.229
GTCGCCC806.9537236E-720.812511
CTAGACT450.00382463220.5555554
TTTGGCG450.00382463220.55555520
AGTCGGT1254.110916E-1019.2411
TCTAGCG609.2328823E-418.528
GTTGTAA609.2328823E-418.51
CGCAATA801.6154941E-518.536
GGATTAA500.007032739518.51
GGTATCA19150.017.2924271
CAGTCGG1502.5102054E-1017.26666610
TAGCACC650.001579267417.0769234
TATTAGA650.001579267417.0769232
CATAACC650.001579267417.0769233
GCGGGTA1201.0398071E-716.95833423
ACGGAGA1005.8725127E-616.65000217
Produced by FastQC (version 0.11.5)