FastQCFastQC Report
Fri 10 Feb 2017
ERR1630437.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630437.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413817
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC19100.4615566784351537No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT14730.35595444363088036No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA14380.3474965987380895No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA12640.30544902698535825No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG11440.27645070163864705No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG11270.27234260554786294No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG10570.2554269157622814No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG8960.21652082925544383No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA8790.21241273316465972No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG8510.2056464572504271No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG8420.2034715828494238No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA8380.20250497200453343No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC7830.18921407288729075No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA7580.1831727551067259No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC7550.18244779697305813No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG7450.1800312698608322No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG7320.17688978461493848No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT7000.1691568978558155No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA6470.15634930416101803No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA6430.15538269331612767No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC6400.1546577351824599No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC6320.15272451349267913No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG6300.15224120807023395No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA6250.15103294451412097No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG5880.142091794198885No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG5780.1396752670866591No Hit
GAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCAT5650.13653378184076537No Hit
GAGTGGGGGTCCCCGCATGCTGCTGTCCCCAGGGAGCTCAGCC5580.1348422128622072No Hit
GGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGCCAGCGT5400.13049246406020054No Hit
TGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGT5390.13025081134897795No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC5380.13000915863775533No Hit
GCTCTCCCCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCC5280.1275926315255294No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA5240.12662602068063902No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC5120.1237261881459679No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG5110.12348453543474532No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC5080.12275957730107753No Hit
GTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTC5080.12275957730107753No Hit
GAGCAAGCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTG5060.12227627187863235No Hit
TCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTG4930.11913478663273863No Hit
GAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACAGAAGG4770.11526834325317713No Hit
CCACTGGAGCCAGTGTACCCAGGGGACAATGCCACACCAGAGC4740.11454338511950936No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT4640.1121268580072834No Hit
GGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTC4630.11188520529606082No Hit
GTAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAG4600.11116024716239303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4450.10753545649405413No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA4400.10632719293794117No Hit
AGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCTCCCTG4340.10487727667060559No Hit
GTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCT4300.10391066582571523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.10318570769204745No Hit
GCTCTGGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGG4220.10197744413593449No Hit
CTTCTGTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCT4160.10052752786859892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGA250.005492329629.62
TACCGCA451.3212136E-424.66666628
GGTATCA3000.022.21
GGATAAG450.00382173320.5555551
TTTATCC450.00382173320.55555533
GCTTTAT3000.019.1166671
TATCAGC500.007027461718.53
TCGTTCG500.007027461718.537
GTATTAG500.007027461718.51
TACTATA500.007027461718.52
CTTACTC500.007027461718.53
CTTTATT3500.016.9142862
TTTATTG3750.016.7733333
CTCACTC1251.650078E-716.2799993
GTCTCAC803.376025E-416.18751
TTAGCAT700.002588736615.85714221
TTATAGT700.002588736615.8571424
GTACTAT700.002588736615.8571421
GTATCAA4200.015.8571422
CGTAACT700.002588736615.85714212
Produced by FastQC (version 0.11.5)