##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630437.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413817
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.725366526749745	31.0	31.0	34.0	30.0	34.0
2	31.910042361720276	33.0	31.0	34.0	30.0	34.0
3	32.062549387772854	33.0	31.0	34.0	30.0	34.0
4	35.72767189361481	37.0	35.0	37.0	33.0	37.0
5	35.638451296104314	37.0	35.0	37.0	33.0	37.0
6	35.71917780081534	37.0	35.0	37.0	33.0	37.0
7	35.686813253201294	37.0	35.0	37.0	33.0	37.0
8	35.65962249013453	37.0	35.0	37.0	33.0	37.0
9	37.35066708230933	39.0	37.0	39.0	34.0	39.0
10	37.270346554153164	39.0	37.0	39.0	34.0	39.0
11	37.347663339108834	39.0	37.0	39.0	34.0	39.0
12	37.214679435595926	39.0	37.0	39.0	34.0	39.0
13	37.254049495308315	39.0	37.0	39.0	34.0	39.0
14	38.444814978601656	40.0	38.0	41.0	34.0	41.0
15	38.446175483365835	40.0	38.0	41.0	34.0	41.0
16	38.419434194341946	40.0	38.0	41.0	34.0	41.0
17	38.39079593153495	40.0	38.0	41.0	34.0	41.0
18	38.359562318609434	40.0	38.0	41.0	34.0	41.0
19	38.341663102289175	40.0	38.0	41.0	34.0	41.0
20	38.31120519456668	40.0	38.0	41.0	34.0	41.0
21	38.252237099974145	40.0	38.0	41.0	34.0	41.0
22	38.215090245205005	40.0	38.0	41.0	34.0	41.0
23	38.195272306357644	40.0	38.0	41.0	34.0	41.0
24	38.170913713066405	40.0	38.0	41.0	33.0	41.0
25	38.14048722019637	40.0	38.0	41.0	33.0	41.0
26	37.97926136432288	40.0	37.0	41.0	33.0	41.0
27	37.869120891601845	40.0	37.0	41.0	33.0	41.0
28	37.78890910716573	40.0	37.0	41.0	33.0	41.0
29	37.738824166237734	40.0	37.0	41.0	32.0	41.0
30	37.69695783401842	40.0	37.0	41.0	32.0	41.0
31	37.569759096412184	40.0	37.0	41.0	32.0	41.0
32	37.52066976465442	40.0	37.0	41.0	32.0	41.0
33	37.44645096745663	40.0	37.0	41.0	32.0	41.0
34	37.367532025025554	39.0	37.0	41.0	31.0	41.0
35	37.28153507468277	39.0	36.0	41.0	31.0	41.0
36	37.183808301737244	39.0	36.0	41.0	31.0	41.0
37	37.13296698782312	39.0	36.0	41.0	31.0	41.0
38	37.02944779938959	39.0	36.0	41.0	31.0	41.0
39	36.94284671726874	39.0	36.0	41.0	30.0	41.0
40	36.84299098393734	39.0	36.0	40.0	30.0	41.0
41	36.72337047535505	39.0	35.0	40.0	30.0	41.0
42	36.69210061452284	39.0	35.0	40.0	30.0	41.0
43	35.42319914358279	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	4.0
16	0.0
17	7.0
18	8.0
19	27.0
20	50.0
21	138.0
22	219.0
23	392.0
24	665.0
25	1102.0
26	1784.0
27	2555.0
28	3649.0
29	5065.0
30	6841.0
31	9229.0
32	11712.0
33	15409.0
34	20186.0
35	27037.0
36	37467.0
37	55726.0
38	102513.0
39	112030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61706503116112	17.799897055945017	11.614795912202737	25.968242000691127
2	20.56247085064171	19.74834286653279	30.67926160597559	29.009924676849913
3	22.4362459734617	20.735494191877084	27.696058885932672	29.132200948728542
4	16.293192401472147	13.508144904631758	32.42036939033437	37.778293303561725
5	16.32533221206475	34.80403173383404	33.53535499991543	15.335281054185787
6	38.99211487203281	31.772740124257826	13.788462049649967	15.446682954059405
7	32.19949881227692	27.86473247836604	19.48904950739095	20.446719201966086
8	30.843343796895727	29.229103685928802	18.86848534497133	21.059067172204138
9	27.91547954772279	13.123192135654167	18.10317120852937	40.85815710809367
10	19.10433839112458	25.222985039280648	31.34283995099283	24.32983661860194
11	38.28914713508628	20.524048069557317	19.11013805619392	22.07666673916248
12	25.54293322893936	23.881087533861585	25.755587614815244	24.82039162238381
13	33.98845383345778	17.705410845856985	22.32049432478608	25.98564099589915
14	24.873313566141555	18.810488694277904	22.595978415579836	33.7202193240007
15	27.698717065756117	26.514135475342965	19.77057491596527	26.016572542935645
16	27.023781043311416	24.51397598455356	21.567262823905253	26.894980148229774
17	26.851482660209708	23.86658837118823	22.45775306476051	26.824175903841553
18	25.676567178245456	22.93864196009347	24.618369955801718	26.766420905859352
19	28.150607635742368	22.64237573613457	24.557956777996072	24.64905985012699
20	29.163857453898707	22.349009344710343	22.33475183474821	26.15238136664274
21	27.65666949400339	22.336926709149214	23.383524601454265	26.622879195393136
22	27.56846625440714	22.946374846852592	24.05386922238574	25.431289676354524
23	27.188104886942778	22.727195837773703	22.443978860220824	27.6407204150627
24	26.612004823388112	23.119156535376746	24.598312780770243	25.670525860464892
25	27.154273507371617	23.69042354470696	22.675482157572066	26.479820790349358
26	27.383360277610635	23.383524601454265	22.973923255931968	26.259191865003128
27	27.118508906110673	21.647491524031153	23.811974858451922	27.42202471140625
28	26.56754072452316	23.28420533714178	22.62159360296943	27.526660335365634
29	26.96578439261799	23.58989601683836	23.895345043823717	25.548974546719926
30	27.565808074583693	23.044969153031413	22.044285275858652	27.344937496526246
31	25.657959919481314	24.538141255675818	24.090358781780353	25.713540043062512
32	25.66182636286088	23.059468315704766	23.418080939159097	27.860624382275255
33	24.9165694014504	24.324278606243823	23.847256154290424	26.911895838015354
34	28.019873518970943	21.55759671545635	23.755669776737058	26.666859988835647
35	26.181862997411898	22.489651222641893	23.484777087456532	27.843708692489678
36	25.304663655673888	23.915643871566417	24.707298153531635	26.072394319228064
37	26.547000244069242	22.01069554899871	25.081134897792985	26.361169309139065
38	26.964092823639437	23.058501704859875	23.3625008155779	26.61490465592279
39	25.870130999934755	22.167769811293397	24.897478837263815	27.064620351508033
40	24.95958358404802	21.516515754548507	25.33946164608994	28.184439015313533
41	23.47341941002907	22.39081526375185	26.402975228180576	27.732790098038507
42	24.71237286046731	21.58393686097961	27.134457985051363	26.569232293501717
43	25.448205366140105	21.73931955429574	26.329512803968903	26.482962275595252
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19.0
1	16.5
2	14.0
3	49.0
4	84.0
5	84.0
6	104.5
7	125.0
8	112.5
9	100.0
10	129.0
11	158.0
12	158.0
13	315.5
14	473.0
15	708.0
16	943.0
17	937.0
18	931.0
19	931.0
20	1196.5
21	1462.0
22	1549.0
23	1636.0
24	2118.5
25	2601.0
26	2601.0
27	3165.5
28	3730.0
29	4517.0
30	5304.0
31	6327.5
32	7351.0
33	7351.0
34	8395.0
35	9439.0
36	10735.0
37	12031.0
38	13220.5
39	14410.0
40	14410.0
41	15448.0
42	16486.0
43	17084.0
44	17682.0
45	19571.0
46	21460.0
47	21460.0
48	24561.5
49	27663.0
50	28709.0
51	29755.0
52	30747.0
53	31739.0
54	31739.0
55	30758.0
56	29777.0
57	31149.0
58	32521.0
59	34192.5
60	35864.0
61	35864.0
62	33455.0
63	31046.0
64	29088.0
65	27130.0
66	23446.0
67	19762.0
68	19762.0
69	17948.5
70	16135.0
71	13024.5
72	9914.0
73	6461.0
74	3008.0
75	3008.0
76	2188.5
77	1369.0
78	1070.5
79	772.0
80	601.0
81	430.0
82	430.0
83	325.0
84	220.0
85	178.0
86	136.0
87	104.5
88	73.0
89	73.0
90	62.5
91	52.0
92	29.5
93	7.0
94	5.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	413817.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.21680528119795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.20307488885618	54.31740996347568
2	5.92467903806034	7.1353048797694925
3	1.3910271777684662	2.5128963811361413
4	0.6314770957179053	1.5210213324952602
5	0.3626239495265542	1.0918027879469732
6	0.23978764982508227	0.8663547731011837
7	0.1678313796040136	0.707438865398279
8	0.12902093585251484	0.6215382857143055
9	0.10861109990829229	0.5886192109098934
>10	0.6465859892918033	7.49669757546314
>50	0.07835340563779905	3.267892207746781
>100	0.10165852116020078	12.732342940562319
>500	0.012456182434649108	4.863124943224546
>1k	0.002812686356211089	2.277555853056005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1910	0.4615566784351537	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1473	0.35595444363088036	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1438	0.3474965987380895	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1264	0.30544902698535825	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	1144	0.27645070163864705	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1127	0.27234260554786294	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	1057	0.2554269157622814	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	896	0.21652082925544383	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	879	0.21241273316465972	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	851	0.2056464572504271	No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG	842	0.2034715828494238	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	838	0.20250497200453343	No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC	783	0.18921407288729075	No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA	758	0.1831727551067259	No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC	755	0.18244779697305813	No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG	745	0.1800312698608322	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	732	0.17688978461493848	No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT	700	0.1691568978558155	No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA	647	0.15634930416101803	No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA	643	0.15538269331612767	No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC	640	0.1546577351824599	No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC	632	0.15272451349267913	No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG	630	0.15224120807023395	No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA	625	0.15103294451412097	No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG	588	0.142091794198885	No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG	578	0.1396752670866591	No Hit
GAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCAT	565	0.13653378184076537	No Hit
GAGTGGGGGTCCCCGCATGCTGCTGTCCCCAGGGAGCTCAGCC	558	0.1348422128622072	No Hit
GGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGCCAGCGT	540	0.13049246406020054	No Hit
TGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGT	539	0.13025081134897795	No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC	538	0.13000915863775533	No Hit
GCTCTCCCCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCC	528	0.1275926315255294	No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA	524	0.12662602068063902	No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC	512	0.1237261881459679	No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG	511	0.12348453543474532	No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC	508	0.12275957730107753	No Hit
GTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTC	508	0.12275957730107753	No Hit
GAGCAAGCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTG	506	0.12227627187863235	No Hit
TCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTG	493	0.11913478663273863	No Hit
GAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACAGAAGG	477	0.11526834325317713	No Hit
CCACTGGAGCCAGTGTACCCAGGGGACAATGCCACACCAGAGC	474	0.11454338511950936	No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT	464	0.1121268580072834	No Hit
GGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTC	463	0.11188520529606082	No Hit
GTAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAG	460	0.11116024716239303	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	445	0.10753545649405413	No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA	440	0.10632719293794117	No Hit
AGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCTCCCTG	434	0.10487727667060559	No Hit
GTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCT	430	0.10391066582571523	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	427	0.10318570769204745	No Hit
GCTCTGGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGG	422	0.10197744413593449	No Hit
CTTCTGTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCT	416	0.10052752786859892	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	2.4165271122259356E-4	0.0
14	0.0	0.0	0.0	2.4165271122259356E-4	0.0
15	0.0	0.0	0.0	2.4165271122259356E-4	0.0
16	0.0	0.0	0.0	4.8330542244518713E-4	0.0
17	0.0	0.0	0.0	4.8330542244518713E-4	0.0
18	0.0	0.0	0.0	4.8330542244518713E-4	0.0
19	0.0	0.0	0.0	7.249581336677807E-4	0.0
20	0.0	0.0	0.0	9.666108448903743E-4	0.0
21	0.0	0.0	0.0	0.0019332216897807485	0.0
22	0.0	0.0	0.0	0.0031414852458937163	0.0
23	0.0	0.0	0.0	0.0065246232030100266	0.0
24	0.0	0.0	0.0	0.01014941387134893	0.0
25	0.0	0.0	0.0	0.010632719293794116	0.0
26	0.0	0.0	0.0	0.013290899117242646	0.0
27	0.0	0.0	0.0	0.021990396721256016	0.0
28	0.0	0.0	0.0	0.05219698562408021	0.0
29	0.0	0.0	0.0	0.1012524860022667	0.0
30	0.0	0.0	0.0	0.15900748398446657	0.0
31	0.0	0.0	0.0	0.3342056996208469	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGA	25	0.0054923296	29.6	2
TACCGCA	45	1.3212136E-4	24.666666	28
GGTATCA	300	0.0	22.2	1
GGATAAG	45	0.003821733	20.555555	1
TTTATCC	45	0.003821733	20.555555	33
GCTTTAT	300	0.0	19.116667	1
TATCAGC	50	0.0070274617	18.5	3
TCGTTCG	50	0.0070274617	18.5	37
GTATTAG	50	0.0070274617	18.5	1
TACTATA	50	0.0070274617	18.5	2
CTTACTC	50	0.0070274617	18.5	3
CTTTATT	350	0.0	16.914286	2
TTTATTG	375	0.0	16.773333	3
CTCACTC	125	1.650078E-7	16.279999	3
GTCTCAC	80	3.376025E-4	16.1875	1
TTAGCAT	70	0.0025887366	15.857142	21
TTATAGT	70	0.0025887366	15.857142	4
GTACTAT	70	0.0025887366	15.857142	1
GTATCAA	420	0.0	15.857142	2
CGTAACT	70	0.0025887366	15.857142	12
>>END_MODULE