FastQCFastQC Report
Fri 10 Feb 2017
ERR1630436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2831015
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC122520.43277764335406205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT111060.39229746221761447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101180.3573983182710088No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77650.2742832517665926No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT60300.21299781173889928No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43430.15340787668027192No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG42340.14955766747968485No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT40520.1431288778053101No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG40180.14192789511888845No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT39700.14023239014982258No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG38570.13624088886848004No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG35490.12536139865030738No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT32860.116071444340634No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG32240.11388141708892394No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT29290.10346112613320664No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG28790.10169497512376303No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC28760.1015890060631964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGC28200.020.8617027
AAGACGG4350.020.4137945
TGCATCA28000.020.08571414
CAGGACA30700.019.8257334
ACAGGCT30550.019.3175138
GCATCAG29800.018.99664315
CCAGGAC32650.018.9816233
GCTGCAT30300.018.56105612
GGACAGG33900.018.1725676
CTGCATC31250.018.05613
ATCAAGC31550.017.82567430
TCCAGGA35700.017.774512
CATCAGA33300.017.27777916
AGAGGCC33550.017.0938923
ATCAGAA33900.017.02654817
AGACGGA5250.016.9142866
AAGAGGC34250.016.69051222
GACGGAC4550.016.670337
CAAGCAG33450.016.64723432
CATCAAG34050.016.62555129
Produced by FastQC (version 0.11.5)