##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630436.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2831015
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82392357511352	31.0	31.0	34.0	30.0	34.0
2	32.01687310028382	33.0	31.0	34.0	30.0	34.0
3	32.154610625517705	34.0	31.0	34.0	30.0	34.0
4	35.81069969604541	37.0	35.0	37.0	35.0	37.0
5	35.73941748807407	37.0	35.0	37.0	35.0	37.0
6	35.800150476066	37.0	35.0	37.0	35.0	37.0
7	35.77285178637344	37.0	35.0	37.0	35.0	37.0
8	35.75702106841539	37.0	35.0	37.0	33.0	37.0
9	37.46683398003896	39.0	37.0	39.0	35.0	39.0
10	37.377238905480894	39.0	37.0	39.0	34.0	39.0
11	37.46004913432108	39.0	37.0	39.0	35.0	39.0
12	37.34173008620583	39.0	37.0	39.0	34.0	39.0
13	37.374453685339006	39.0	37.0	39.0	34.0	39.0
14	38.58314102892425	40.0	38.0	41.0	34.0	41.0
15	38.58327737578218	40.0	38.0	41.0	34.0	41.0
16	38.570555790061164	40.0	38.0	41.0	34.0	41.0
17	38.542196350072324	40.0	38.0	41.0	34.0	41.0
18	38.48389040679756	40.0	38.0	41.0	34.0	41.0
19	38.50570060561318	40.0	38.0	41.0	34.0	41.0
20	38.457060806813104	40.0	38.0	41.0	34.0	41.0
21	38.42683348551668	40.0	38.0	41.0	34.0	41.0
22	38.39082131320392	40.0	38.0	41.0	34.0	41.0
23	38.33643304609831	40.0	38.0	41.0	34.0	41.0
24	38.321836867695865	40.0	38.0	41.0	34.0	41.0
25	38.290363703477375	40.0	38.0	41.0	34.0	41.0
26	38.11792166413813	40.0	38.0	41.0	33.0	41.0
27	38.01679327025819	40.0	37.0	41.0	33.0	41.0
28	37.9197863663739	40.0	37.0	41.0	33.0	41.0
29	37.84060769723933	40.0	37.0	41.0	33.0	41.0
30	37.777266457436646	40.0	37.0	41.0	33.0	41.0
31	37.67191131096091	40.0	37.0	41.0	33.0	41.0
32	37.568198684923956	40.0	37.0	41.0	32.0	41.0
33	37.494084983654275	40.0	37.0	41.0	32.0	41.0
34	37.38111454725602	40.0	36.0	41.0	32.0	41.0
35	37.267969968368234	39.0	36.0	41.0	31.0	41.0
36	37.16374303915733	39.0	36.0	41.0	31.0	41.0
37	37.078642818918304	39.0	36.0	41.0	31.0	41.0
38	36.96552826459768	39.0	36.0	41.0	31.0	41.0
39	36.86327483252473	39.0	35.0	41.0	30.0	41.0
40	36.73510242792779	39.0	35.0	41.0	30.0	41.0
41	36.58735294585158	39.0	35.0	41.0	30.0	41.0
42	36.52068251139609	39.0	35.0	41.0	30.0	41.0
43	35.33638500679085	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	3.0
11	3.0
12	9.0
13	6.0
14	9.0
15	10.0
16	19.0
17	62.0
18	133.0
19	234.0
20	507.0
21	1022.0
22	1740.0
23	3080.0
24	4824.0
25	7603.0
26	11592.0
27	16877.0
28	24302.0
29	33238.0
30	44907.0
31	59340.0
32	77282.0
33	101622.0
34	135807.0
35	180462.0
36	247280.0
37	374539.0
38	660704.0
39	843791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80931044166139	18.340241927365273	12.037908665266697	26.812538965706644
2	18.469276920115224	20.131754865304494	35.00995226093821	26.38901595364207
3	19.86658495274663	22.514893068387135	29.53477816260246	28.08374381626378
4	14.553896747279685	15.0382460001095	34.14863573665276	36.25922151595805
5	14.830829225560443	36.7790350810575	33.35150114005047	15.038634553331578
6	35.291582700904094	34.387278061048775	15.553679510705523	14.767459727341606
7	30.085675985468107	30.26532886614871	20.805647444467798	18.84334770391538
8	28.216311111032617	33.46672483190658	19.243663491715868	19.07330056534494
9	27.46919391101778	13.682265901099075	18.969733470151166	39.878806717731976
10	17.696833114624965	26.66202757668186	31.82402777802308	23.817111530670097
11	36.497475287132005	21.559581987379083	20.87505717913893	21.067885546349984
12	22.651416541417124	24.963378858819187	28.6318864435547	23.75331815620899
13	30.375112812895726	18.80601127157574	24.80552028159512	26.01335563393341
14	22.784831588670496	20.064429188824505	25.313288696810154	31.837450525694848
15	25.971815762191298	26.72751645611203	21.792254721363186	25.508413060333485
16	24.908345593364924	25.744158897074016	23.303126263901817	26.044369245659244
17	23.59079694031999	26.44189451486481	25.53706002970666	24.430248515108538
18	23.98383618596157	24.237137563735974	26.591098952142605	25.187927298159845
19	24.699445251967934	24.58899016783733	26.080928571554722	24.63063600864001
20	24.52413710277056	25.089623332974213	25.943875253221897	24.44236431103332
21	26.422219592619605	24.34808717014922	25.18693825359456	24.04275498363661
22	25.542888328037822	24.87839167224476	25.43935655586424	24.139363443853178
23	24.726326070331666	24.908098332223602	25.459243416230574	24.906332181214157
24	24.989305955637818	24.928409068832202	25.534163542051175	24.548121433478805
25	24.911171434980034	24.59941045879305	25.99205585275952	24.497362253467394
26	25.303115666995758	25.073657327848846	25.777786412293825	23.84544059286157
27	25.389056575115287	24.916187303846854	25.04126611833565	24.65349000270221
28	24.27680531540808	24.45889548448171	26.43119163974758	24.833107560362627
29	24.114001515357568	24.735757316722097	26.620840935141636	24.529400232778702
30	23.98422473918365	25.36288221715533	26.230238977893087	24.422654065767933
31	24.15617720146308	25.249354030268297	25.958993505862736	24.635475262405887
32	23.731912405974537	25.0042829161979	26.249030824633568	25.014773853193994
33	24.35059510458263	25.12021306845778	26.02529481475725	24.503897012202337
34	24.877014074457392	25.336178013892546	25.604915551489484	24.181892360160578
35	25.081605007391346	25.029291614491623	26.27778376306731	23.611319615049727
36	24.650169638804456	25.49968827434683	24.876378260093997	24.973763826754716
37	24.130497365785768	24.787717479419925	26.224163418420606	24.8576217363737
38	24.783443393977073	24.714563504608773	26.302262616058197	24.199730485355957
39	23.44840984593865	25.05264013083647	26.554822210408634	24.94412781281625
40	24.094644500294063	24.17337951229506	27.491482736756957	24.240493250653916
41	22.95314577987047	24.367832738434803	27.774738035651524	24.904283446043205
42	23.322377309904752	25.05073268774627	26.882160638498913	24.74472936385007
43	23.277481751244693	23.435552266589898	28.022811606437976	25.264154375727433
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	399.0
1	386.0
2	373.0
3	958.0
4	1543.0
5	1543.0
6	2161.5
7	2780.0
8	2614.0
9	2448.0
10	3385.0
11	4322.0
12	4322.0
13	7875.5
14	11429.0
15	17671.0
16	23913.0
17	22140.5
18	20368.0
19	20368.0
20	25537.0
21	30706.0
22	29693.5
23	28681.0
24	33487.5
25	38294.0
26	38294.0
27	42796.5
28	47299.0
29	54040.0
30	60781.0
31	69474.5
32	78168.0
33	78168.0
34	89153.0
35	100138.0
36	111598.5
37	123059.0
38	130411.5
39	137764.0
40	137764.0
41	145574.5
42	153385.0
43	157473.0
44	161561.0
45	165687.0
46	169813.0
47	169813.0
48	172667.0
49	175521.0
50	181113.0
51	186705.0
52	192629.5
53	198554.0
54	198554.0
55	199701.5
56	200849.0
57	189918.0
58	178987.0
59	164884.5
60	150782.0
61	150782.0
62	143512.0
63	136242.0
64	118866.5
65	101491.0
66	87359.0
67	73227.0
68	73227.0
69	64246.5
70	55266.0
71	49851.5
72	44437.0
73	47847.0
74	51257.0
75	51257.0
76	47944.5
77	44632.0
78	33772.5
79	22913.0
80	14705.5
81	6498.0
82	6498.0
83	4525.5
84	2553.0
85	2127.5
86	1702.0
87	1427.5
88	1153.0
89	1153.0
90	984.0
91	815.0
92	476.5
93	138.0
94	97.0
95	56.0
96	56.0
97	31.5
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2831015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.27156955226925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5169561503989	35.54585841395259
2	12.226536008408164	11.070289505759492
3	3.8502225563605843	5.229168547559823
4	1.632099635768265	2.9555084870766524
5	0.8792748573281193	1.9903076429545787
6	0.5468098073514396	1.4852962935224188
7	0.3613395300196625	1.1450885365688603
8	0.24368051561058204	0.8825439528797838
9	0.1974205293494486	0.8043783502940436
>10	1.2478371117946319	10.939093248579006
>50	0.1504722462772413	4.791024920315543
>100	0.12649476058759662	11.530207924580733
>500	0.013435513139507462	4.279617183954802
>1k	0.007030210363694478	5.679963913537208
>5k	1.562268969709884E-4	0.48783512549804997
>10k+	2.343403454564826E-4	1.1838179529664894
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	12252	0.43277764335406205	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11106	0.39229746221761447	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10118	0.3573983182710088	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7765	0.2742832517665926	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	6030	0.21299781173889928	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4343	0.15340787668027192	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	4234	0.14955766747968485	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	4052	0.1431288778053101	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	4018	0.14192789511888845	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	3970	0.14023239014982258	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	3857	0.13624088886848004	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	3549	0.12536139865030738	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	3286	0.116071444340634	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	3224	0.11388141708892394	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	2929	0.10346112613320664	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	2879	0.10169497512376303	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	2876	0.1015890060631964	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.532302018887219E-5	0.0	0.0	0.0	0.0
3	3.532302018887219E-5	0.0	0.0	0.0	0.0
4	3.532302018887219E-5	0.0	0.0	0.0	0.0
5	7.064604037774438E-5	3.532302018887219E-5	0.0	0.0	0.0
6	7.064604037774438E-5	3.532302018887219E-5	0.0	0.0	0.0
7	7.064604037774438E-5	3.532302018887219E-5	0.0	0.0	0.0
8	7.064604037774438E-5	3.532302018887219E-5	0.0	0.0	3.532302018887219E-5
9	7.064604037774438E-5	3.532302018887219E-5	0.0	0.0	3.532302018887219E-5
10	1.4129208075548875E-4	3.532302018887219E-5	0.0	7.064604037774438E-5	3.532302018887219E-5
11	1.7661510094436095E-4	3.532302018887219E-5	0.0	7.064604037774438E-5	3.532302018887219E-5
12	1.7661510094436095E-4	3.532302018887219E-5	0.0	1.7661510094436095E-4	3.532302018887219E-5
13	2.1193812113323314E-4	3.532302018887219E-5	0.0	2.1193812113323314E-4	3.532302018887219E-5
14	2.1193812113323314E-4	3.532302018887219E-5	0.0	2.472611413221053E-4	3.532302018887219E-5
15	2.472611413221053E-4	3.532302018887219E-5	0.0	4.591992624553385E-4	3.532302018887219E-5
16	2.472611413221053E-4	3.532302018887219E-5	0.0	6.711373835885716E-4	3.532302018887219E-5
17	2.825841615109775E-4	3.532302018887219E-5	0.0	0.0011656596662327821	3.532302018887219E-5
18	2.825841615109775E-4	3.532302018887219E-5	0.0	0.0015542128883103763	3.532302018887219E-5
19	2.825841615109775E-4	3.532302018887219E-5	0.0	0.0019427661103879703	3.532302018887219E-5
20	2.825841615109775E-4	3.532302018887219E-5	0.0	0.0024726114132210533	3.532302018887219E-5
21	2.825841615109775E-4	3.532302018887219E-5	0.0	0.003179071816998497	3.532302018887219E-5
22	3.179071816998497E-4	3.532302018887219E-5	0.0	0.004697961685120001	3.532302018887219E-5
23	3.532302018887219E-4	1.0596906056661657E-4	0.0	0.0068879889368300765	3.532302018887219E-5
24	3.532302018887219E-4	1.0596906056661657E-4	0.0	0.009749153572128725	3.532302018887219E-5
25	3.532302018887219E-4	1.0596906056661657E-4	0.0	0.011374012500816846	3.532302018887219E-5
26	3.532302018887219E-4	1.0596906056661657E-4	0.0	0.014694376398570831	3.532302018887219E-5
27	3.885532220775941E-4	1.0596906056661657E-4	0.0	0.024090299768810833	3.532302018887219E-5
28	4.238762422664663E-4	1.0596906056661657E-4	0.0	0.05104176417292031	3.532302018887219E-5
29	4.238762422664663E-4	1.4129208075548875E-4	0.0	0.08996773242105746	3.532302018887219E-5
30	4.238762422664663E-4	1.4129208075548875E-4	0.0	0.1478268394904301	3.532302018887219E-5
31	4.238762422664663E-4	1.4129208075548875E-4	0.0	0.31578780048851735	3.532302018887219E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAGGC	2820	0.0	20.861702	7
AAGACGG	435	0.0	20.413794	5
TGCATCA	2800	0.0	20.085714	14
CAGGACA	3070	0.0	19.825733	4
ACAGGCT	3055	0.0	19.317513	8
GCATCAG	2980	0.0	18.996643	15
CCAGGAC	3265	0.0	18.981623	3
GCTGCAT	3030	0.0	18.561056	12
GGACAGG	3390	0.0	18.172567	6
CTGCATC	3125	0.0	18.056	13
ATCAAGC	3155	0.0	17.825674	30
TCCAGGA	3570	0.0	17.77451	2
CATCAGA	3330	0.0	17.277779	16
AGAGGCC	3355	0.0	17.09389	23
ATCAGAA	3390	0.0	17.026548	17
AGACGGA	525	0.0	16.914286	6
AAGAGGC	3425	0.0	16.690512	22
GACGGAC	455	0.0	16.67033	7
CAAGCAG	3345	0.0	16.647234	32
CATCAAG	3405	0.0	16.625551	29
>>END_MODULE