FastQCFastQC Report
Fri 10 Feb 2017
ERR1630435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808107
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG13370.16544838740414325No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT11790.14589652112900892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11710.14490655321634388No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG10850.13426439815519478No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG10510.1300570345263684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10490.12980954254820215No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA9320.11533126182547608No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC9280.11483627786914358No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA9010.1114951361638991No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT8940.11062891424031718No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA8610.10654529660057394No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG8520.10543158269882577No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC8140.10072923511366687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT502.7011396E-422.26
TACCGTC603.724524E-521.5833327
TAACCCG1301.8189894E-1221.34615328
GGTATCA5900.021.3220331
CGGGTAA1351.8189894E-1220.55555524
GCGGGTA1351.8189894E-1220.55555523
ATCGAAC851.2443725E-619.5882341
CGCGGGT1457.2759576E-1219.1379322
AACCCGT1850.019.00000229
TAGAACA701.2186292E-418.54
CCGTACA801.6157022E-518.517
TATACTG1002.8722934E-718.55
ATACCGT801.6157022E-518.56
GTAACCC1501.2732926E-1118.527
TCTATAC609.233581E-418.53
CCCGTTG1900.018.49999831
ACCCGTT1900.018.49999830
CCGTTGA1900.018.49999832
TTATGTC752.0663095E-417.2666664
ACCGTCG752.0663095E-417.2666668
Produced by FastQC (version 0.11.5)