##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630435.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	808107
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.041584839631387	31.0	31.0	34.0	28.0	34.0
2	31.266900299094054	31.0	31.0	34.0	28.0	34.0
3	31.410715412686685	31.0	31.0	34.0	28.0	34.0
4	35.20516713752016	37.0	35.0	37.0	32.0	37.0
5	34.954496124894355	35.0	35.0	37.0	32.0	37.0
6	35.019961465499	36.0	35.0	37.0	32.0	37.0
7	34.99868458013604	36.0	35.0	37.0	32.0	37.0
8	34.96233295838299	36.0	35.0	37.0	32.0	37.0
9	36.57933540979103	38.0	35.0	39.0	32.0	39.0
10	36.35463868027377	38.0	35.0	39.0	32.0	39.0
11	36.49421178136064	38.0	35.0	39.0	32.0	39.0
12	36.30244014715873	38.0	35.0	39.0	32.0	39.0
13	36.39054234154636	38.0	35.0	39.0	32.0	39.0
14	37.401229045163575	39.0	36.0	40.0	32.0	41.0
15	37.35275279140015	39.0	36.0	40.0	32.0	41.0
16	37.326383758586424	39.0	36.0	40.0	32.0	41.0
17	37.23256078712349	39.0	36.0	40.0	32.0	41.0
18	37.189318988698275	39.0	36.0	40.0	32.0	41.0
19	37.182269179700214	39.0	36.0	40.0	32.0	41.0
20	37.11413340065115	39.0	36.0	40.0	31.0	41.0
21	37.0510031468605	39.0	36.0	40.0	31.0	41.0
22	37.01153065126277	39.0	36.0	40.0	31.0	41.0
23	36.954757228931314	39.0	36.0	40.0	31.0	41.0
24	36.922156348107364	39.0	36.0	40.0	31.0	41.0
25	36.87868561960236	39.0	36.0	40.0	31.0	41.0
26	36.66772964471289	38.0	36.0	40.0	30.0	41.0
27	36.52464958229542	38.0	35.0	40.0	30.0	41.0
28	36.426478176776094	38.0	35.0	40.0	30.0	41.0
29	36.319299300711414	38.0	35.0	40.0	30.0	41.0
30	36.24374989945638	38.0	35.0	40.0	30.0	41.0
31	36.09178611248263	38.0	35.0	40.0	29.0	41.0
32	35.97487090199689	38.0	35.0	40.0	29.0	41.0
33	35.91713721079015	38.0	35.0	40.0	29.0	41.0
34	35.76985473458342	38.0	34.0	40.0	28.0	41.0
35	35.68188742332389	38.0	34.0	40.0	28.0	41.0
36	35.56194786086496	38.0	34.0	40.0	27.0	41.0
37	35.465808364486385	38.0	34.0	40.0	27.0	41.0
38	35.3759056659576	38.0	34.0	40.0	27.0	41.0
39	35.22473261585409	38.0	34.0	40.0	26.0	41.0
40	35.109148912210884	38.0	33.0	40.0	26.0	41.0
41	34.965219952308296	38.0	33.0	40.0	26.0	41.0
42	34.90548900083776	38.0	33.0	40.0	26.0	41.0
43	33.60440882209905	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	1.0
12	1.0
13	3.0
14	4.0
15	10.0
16	25.0
17	40.0
18	107.0
19	164.0
20	352.0
21	668.0
22	1171.0
23	1851.0
24	3155.0
25	4707.0
26	6677.0
27	9481.0
28	12763.0
29	17013.0
30	21790.0
31	27961.0
32	35284.0
33	44404.0
34	57113.0
35	75653.0
36	103327.0
37	144147.0
38	176996.0
39	63236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69372743955937	19.357708818262928	12.230682323009205	25.717881419168503
2	19.942903600637045	21.074065686845923	33.00961382589187	25.973416886625163
3	21.222684619734764	21.588849001431743	28.941711926762174	28.246754452071322
4	15.123368563816426	15.63097461103542	33.8888290783275	35.356827746820656
5	15.78602833535658	35.65555056446733	33.44111608982474	15.11730501035135
6	36.844749519556196	34.12270899769461	15.005191144242037	14.027350338507153
7	31.71287960628976	29.280404698882695	19.58960880180471	19.41710689302283
8	28.86610312743238	32.58825873306381	19.19943769822561	19.346200441278196
9	27.58582712437833	12.974519463387892	18.403379750453837	41.03627366177994
10	17.340649196207927	25.971189458821662	32.590981144823644	24.097180200146763
11	38.47126680006484	20.622887810648837	19.699371494121447	21.206473895164873
12	22.67335884975628	24.3983779375751	28.15208877042273	24.77617444224589
13	32.10552562965053	18.324677301396967	23.69451075167026	25.875286317282242
14	23.999173376792925	19.662618935363756	23.670751521766302	32.66745616607702
15	26.30839727907319	26.66750813939243	21.378975803946755	25.645118777587623
16	27.248743050115888	24.887174594453455	22.416338430430624	25.447743925000033
17	25.10162639353452	24.94385025745353	23.768139615174725	26.186383733837225
18	24.994338620999446	23.529681094211533	25.60069396750678	25.875286317282242
19	26.7469530643838	24.338856116826115	24.516926595116736	24.39726422367335
20	27.080077266995584	23.53586839366569	24.277230614262717	25.106823725076012
21	26.86760540373985	23.9934810612951	23.560246353515065	25.578667181449983
22	27.297746461792805	23.468426829615385	24.02627374840213	25.207552960189677
23	25.245171740870948	23.87010631017922	24.34430094034577	26.54042100860406
24	25.812299608838927	24.378083595365467	24.22439107692422	25.585225718871385
25	26.183537576088316	24.02553127246763	24.20063184702026	25.590299304423798
26	26.562200302682687	24.251243956555257	23.30718580584007	25.879369934921986
27	26.613802380130352	23.65293209933833	23.553316578126413	26.179948942404906
28	25.79757383613804	24.113143432738486	24.319922980496393	25.769359750627086
29	25.78507549124064	24.51952526088748	24.83495378706038	24.8604454608115
30	26.31879194215617	24.28341791371687	23.710845222229235	25.68694492189772
31	25.773072130299578	24.093467820474267	23.974671670954464	26.15878837827169
32	24.968475709281073	24.505665710110172	24.154722085070418	26.371136495538337
33	25.439947927687793	23.64179496032085	24.69041847181128	26.227838640180074
34	26.337848824474975	23.39739663188167	24.9536261905911	25.311128353052258
35	26.572223727798423	23.583510599462695	24.2956687666361	25.548596906102784
36	25.22660984250848	24.342073512542274	23.775316882541546	26.6559997624077
37	25.426583360866818	23.838303590984857	25.391192007989037	25.343921040159284
38	25.248017898619864	23.359654105211312	24.852649463499265	26.53967853266956
39	24.91699737782249	23.72099239333405	25.85585819699619	25.506152031847268
40	25.57594476969015	23.012794097811305	25.65489471072519	25.756366421773354
41	24.25495633622775	23.386878222809603	26.39699940725671	25.961166033705936
42	24.529177448035966	23.680403708914785	26.161758282009686	25.628660561039567
43	24.352963159581588	22.466331810020208	26.044323338369797	27.136381692028404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	17.5
2	18.0
3	58.0
4	98.0
5	98.0
6	144.0
7	190.0
8	195.5
9	201.0
10	279.0
11	357.0
12	357.0
13	673.0
14	989.0
15	1637.0
16	2285.0
17	2279.0
18	2273.0
19	2273.0
20	2769.0
21	3265.0
22	3521.0
23	3777.0
24	4720.0
25	5663.0
26	5663.0
27	6817.0
28	7971.0
29	9757.5
30	11544.0
31	13796.5
32	16049.0
33	16049.0
34	20618.5
35	25188.0
36	28318.5
37	31449.0
38	33462.0
39	35475.0
40	35475.0
41	37931.5
42	40388.0
43	43225.0
44	46062.0
45	47965.5
46	49869.0
47	49869.0
48	51794.0
49	53719.0
50	55809.5
51	57900.0
52	62804.5
53	67709.0
54	67709.0
55	67286.5
56	66864.0
57	62899.5
58	58935.0
59	58209.5
60	57484.0
61	57484.0
62	52960.0
63	48436.0
64	40424.5
65	32413.0
66	28634.0
67	24855.0
68	24855.0
69	22086.0
70	19317.0
71	18525.5
72	17734.0
73	14973.0
74	12212.0
75	12212.0
76	7735.0
77	3258.0
78	2537.0
79	1816.0
80	1444.0
81	1072.0
82	1072.0
83	820.5
84	569.0
85	455.0
86	341.0
87	259.0
88	177.0
89	177.0
90	154.0
91	131.0
92	78.0
93	25.0
94	17.0
95	9.0
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	808107.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.02363556278489
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.67771451620911	56.77469676726451
2	7.253321777912837	9.28768060257405
3	1.6555936525485921	3.179913739524929
4	0.6892503000495227	1.765132400876431
5	0.3622188956406131	1.1595285284224512
6	0.22939464704859014	0.8812007569615563
7	0.16665157452678164	0.7468747771426868
8	0.12189097343480379	0.6243122609266377
9	0.086413719014661	0.49792684084375954
>10	0.5929108913546691	7.455251717119535
>50	0.080865808034618	3.714125059564292
>100	0.07504686462352543	9.879209453502424
>500	0.0075628623214817085	3.1809584442214143
>1k	0.0011635172802279552	0.8531886510553671
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	1337	0.16544838740414325	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	1179	0.14589652112900892	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1171	0.14490655321634388	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1085	0.13426439815519478	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1051	0.1300570345263684	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1049	0.12980954254820215	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	932	0.11533126182547608	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	928	0.11483627786914358	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	901	0.1114951361638991	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	894	0.11062891424031718	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	861	0.10654529660057394	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	852	0.10543158269882577	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	814	0.10072923511366687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2374598908312885E-4
2	0.0	0.0	0.0	0.0	1.2374598908312885E-4
3	0.0	0.0	0.0	0.0	1.2374598908312885E-4
4	0.0	0.0	0.0	0.0	1.2374598908312885E-4
5	1.2374598908312885E-4	0.0	0.0	0.0	1.2374598908312885E-4
6	1.2374598908312885E-4	0.0	0.0	0.0	1.2374598908312885E-4
7	1.2374598908312885E-4	0.0	0.0	0.0	1.2374598908312885E-4
8	1.2374598908312885E-4	0.0	0.0	0.0	1.2374598908312885E-4
9	1.2374598908312885E-4	0.0	0.0	0.0	1.2374598908312885E-4
10	1.2374598908312885E-4	0.0	0.0	1.2374598908312885E-4	1.2374598908312885E-4
11	1.2374598908312885E-4	0.0	0.0	1.2374598908312885E-4	1.2374598908312885E-4
12	1.2374598908312885E-4	0.0	0.0	1.2374598908312885E-4	1.2374598908312885E-4
13	1.2374598908312885E-4	0.0	0.0	2.474919781662577E-4	1.2374598908312885E-4
14	1.2374598908312885E-4	0.0	0.0	2.474919781662577E-4	1.2374598908312885E-4
15	2.474919781662577E-4	0.0	0.0	4.949839563325154E-4	1.2374598908312885E-4
16	2.474919781662577E-4	0.0	0.0	8.662219235819018E-4	1.2374598908312885E-4
17	2.474919781662577E-4	0.0	0.0	0.0011137139017481595	1.2374598908312885E-4
18	2.474919781662577E-4	0.0	0.0	0.0014849518689975462	1.2374598908312885E-4
19	2.474919781662577E-4	0.0	0.0	0.0014849518689975462	1.2374598908312885E-4
20	2.474919781662577E-4	0.0	0.0	0.001608697858080675	1.2374598908312885E-4
21	2.474919781662577E-4	0.0	0.0	0.0018561898362469327	2.474919781662577E-4
22	2.474919781662577E-4	0.0	0.0	0.0027224117598288345	2.474919781662577E-4
23	2.474919781662577E-4	0.0	0.0	0.0037123796724938653	2.474919781662577E-4
24	2.474919781662577E-4	0.0	0.0	0.005444823519657669	2.474919781662577E-4
25	2.474919781662577E-4	0.0	0.0	0.0064347914323227	2.474919781662577E-4
26	2.474919781662577E-4	0.0	0.0	0.00841472725765276	2.474919781662577E-4
27	2.474919781662577E-4	0.0	0.0	0.013240820831894786	2.474919781662577E-4
28	2.474919781662577E-4	0.0	0.0	0.035886336834107364	2.474919781662577E-4
29	2.474919781662577E-4	0.0	0.0	0.06707032608305584	2.474919781662577E-4
30	2.474919781662577E-4	0.0	0.0	0.1093914543494859	2.474919781662577E-4
31	2.474919781662577E-4	0.0	0.0	0.27347863587371474	2.474919781662577E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCGT	50	2.7011396E-4	22.2	6
TACCGTC	60	3.724524E-5	21.583332	7
TAACCCG	130	1.8189894E-12	21.346153	28
GGTATCA	590	0.0	21.322033	1
CGGGTAA	135	1.8189894E-12	20.555555	24
GCGGGTA	135	1.8189894E-12	20.555555	23
ATCGAAC	85	1.2443725E-6	19.588234	1
CGCGGGT	145	7.2759576E-12	19.13793	22
AACCCGT	185	0.0	19.000002	29
TAGAACA	70	1.2186292E-4	18.5	4
CCGTACA	80	1.6157022E-5	18.5	17
TATACTG	100	2.8722934E-7	18.5	5
ATACCGT	80	1.6157022E-5	18.5	6
GTAACCC	150	1.2732926E-11	18.5	27
TCTATAC	60	9.233581E-4	18.5	3
CCCGTTG	190	0.0	18.499998	31
ACCCGTT	190	0.0	18.499998	30
CCGTTGA	190	0.0	18.499998	32
TTATGTC	75	2.0663095E-4	17.266666	4
ACCGTCG	75	2.0663095E-4	17.266666	8
>>END_MODULE