FastQCFastQC Report
Fri 10 Feb 2017
ERR1630434.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630434.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352685
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94802.6879510044373873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71072.0151126359215734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44631.265435161688192No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25070.710832612671364No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12050.34166465826445697No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.25830415243063926No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8970.25433460453379075No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7130.20216340360378243No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6590.1868522902873669No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6570.18628521201638856No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT6170.17494364659682152No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6090.17267533351290812No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5930.1681387073450813No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5840.16558685512567872No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5790.16416915944823285No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5760.1633185420417653No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA5700.16161730722883025No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5600.1587819158739385No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5290.14999220267377403No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.14630619391241476No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5130.14545557650594723No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5090.14432141996399053No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.1420531068800771No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4940.14006833293165288No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4490.12730907183463996No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4440.1258913761571941No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4160.11795228036349717No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3950.11199795851822447No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3930.11143088024724614No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.1105802628407786No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA3890.11029672370528942No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3860.1094461062988219No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA3680.10434240186001673No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA3610.1023576279115925No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.10065639309865743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC250.005491243729.5999983
GATCGCA250.005491243729.59999810
TGATCAC358.8571565E-426.42857221
TTGATCA358.8571565E-426.42857220
ACGTTTA358.8571565E-426.42857226
TCACGTT358.8571565E-426.42857224
GTGGTTT400.001928548423.12537
TATTGTA450.003820629620.55555531
TCAAGAC555.1333837E-420.1818183
TTGTAAG500.007025453318.49999833
TATCTGT500.007025453318.49999833
TCTATGT500.007025453318.49999829
CAGTCGG852.7144575E-517.41176410
TGGACAA752.0621512E-417.2666665
TCTTATA2250.017.26666637
AGTTTTT650.001576999817.07692315
GGGTTAG650.001576999817.0769237
GTACTGG2350.016.5319161
CTAGAAA904.4319117E-516.44444529
GCAGTCG904.4319117E-516.4444459
Produced by FastQC (version 0.11.5)