##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630434.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	352685
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.529126557693125	31.0	31.0	34.0	30.0	34.0
2	31.65882019365723	31.0	31.0	34.0	30.0	34.0
3	31.67779746799552	31.0	31.0	34.0	28.0	34.0
4	35.441544154131876	37.0	35.0	37.0	33.0	37.0
5	35.399863901214964	37.0	35.0	37.0	33.0	37.0
6	35.51827835036931	37.0	35.0	37.0	33.0	37.0
7	35.522055091654025	37.0	35.0	37.0	33.0	37.0
8	35.56234316741568	37.0	35.0	37.0	33.0	37.0
9	37.11787856018827	39.0	37.0	39.0	33.0	39.0
10	37.01709457447864	39.0	37.0	39.0	33.0	39.0
11	37.15095907112579	39.0	37.0	39.0	33.0	39.0
12	37.01623261550675	39.0	37.0	39.0	33.0	39.0
13	37.10472234430157	39.0	37.0	39.0	33.0	39.0
14	38.071766590583664	40.0	37.0	41.0	33.0	41.0
15	38.09804216226945	40.0	37.0	41.0	33.0	41.0
16	37.92779959453904	40.0	37.0	41.0	33.0	41.0
17	38.07228263181025	40.0	37.0	41.0	33.0	41.0
18	38.11190439060351	40.0	37.0	41.0	33.0	41.0
19	38.1584133149978	40.0	37.0	41.0	34.0	41.0
20	38.15186923175071	40.0	38.0	41.0	34.0	41.0
21	38.12597076711513	40.0	37.0	41.0	33.0	41.0
22	38.07344797765712	40.0	37.0	41.0	33.0	41.0
23	38.0101138409629	40.0	37.0	41.0	33.0	41.0
24	37.96509066163857	40.0	37.0	41.0	33.0	41.0
25	37.91058309823213	40.0	37.0	41.0	33.0	41.0
26	37.63103335837929	39.0	37.0	41.0	33.0	41.0
27	37.448964373307625	39.0	36.0	41.0	32.0	41.0
28	37.33882359612686	39.0	36.0	41.0	32.0	41.0
29	37.203680337978646	39.0	36.0	40.0	32.0	41.0
30	36.98194422785205	39.0	35.0	40.0	31.0	41.0
31	36.72654918695153	38.0	35.0	40.0	31.0	41.0
32	36.46926577540865	38.0	35.0	40.0	31.0	41.0
33	36.2776727107759	38.0	35.0	40.0	30.0	41.0
34	36.09437033046486	38.0	35.0	40.0	30.0	41.0
35	35.90740745991465	38.0	35.0	40.0	30.0	41.0
36	35.62882175312247	38.0	35.0	40.0	29.0	41.0
37	35.4060847498476	38.0	35.0	40.0	27.0	41.0
38	35.14058153876689	38.0	34.0	40.0	26.0	41.0
39	34.876050866920906	38.0	34.0	40.0	25.0	41.0
40	34.55054510398798	37.0	33.0	40.0	23.0	41.0
41	34.217253356394515	37.0	33.0	40.0	22.0	41.0
42	33.91344400810922	37.0	33.0	40.0	20.0	41.0
43	32.85817655981967	36.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	3.0
12	2.0
13	3.0
14	1.0
15	5.0
16	2.0
17	11.0
18	20.0
19	51.0
20	111.0
21	194.0
22	353.0
23	668.0
24	999.0
25	1635.0
26	2442.0
27	3743.0
28	4842.0
29	6429.0
30	8221.0
31	10286.0
32	12907.0
33	16378.0
34	21127.0
35	27902.0
36	38714.0
37	60857.0
38	79905.0
39	54867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.94917560996357	21.461077165175723	13.487956675220097	20.101790549640615
2	19.55654479209493	25.030551341848962	32.28234827112012	23.13055559493599
3	22.71828969193473	25.703106171229283	31.130895841898575	20.44770829493741
4	14.889490621943095	19.032564469710934	33.128996129690805	32.94894877865517
5	13.204134000595433	39.489062477848506	32.466648709188085	14.840154812367977
6	29.477012064590213	34.45255681415427	17.49152926832726	18.57890185292825
7	24.856174773523115	34.583268355614784	21.507861122531438	19.052695748330663
8	28.873924323404736	32.178005869260105	20.628039185108523	18.320030622226632
9	26.998312942143837	12.921445482512725	22.772445666813162	37.307795908530274
10	21.673164438521628	28.740660929724825	28.723648581595473	20.862526050158074
11	31.775380296865475	24.525284602407247	19.79641890071877	23.902916200008505
12	19.42271432014404	31.127209833137222	28.472999985823044	20.9770758608957
13	31.78048400130428	20.454796773324638	26.848037200334574	20.916682025036508
14	21.9226788777521	24.627075152047862	29.214738364262725	24.235507605937308
15	28.854360123055983	26.369423139628843	25.115046004224734	19.661170733090437
16	20.97395693040532	26.290599259962853	29.81669194890625	22.91875186072558
17	20.163885620312744	29.51302153479734	28.77326793030608	21.549824914583834
18	19.00874718232984	25.299913520563678	31.6803946864766	24.010944610629885
19	19.949246494747438	27.549229482399305	33.56819825056353	18.93332577228972
20	20.651572933354124	24.904376426556276	35.030409572281215	19.413641067808385
21	22.100174376568326	25.779945276946854	32.78959978451026	19.33028056197457
22	21.597459489346015	26.716758580603088	32.19530175652495	19.490480173525953
23	21.465330252208062	26.76127422487489	32.29765938443654	19.475736138480514
24	20.015311113316418	27.895147227696103	32.208344556757446	19.881197102230036
25	20.242993039114225	27.949019663439046	32.53101209294413	19.2769752045026
26	20.583523540836723	27.958092915774703	32.26675361866821	19.19162992472036
27	19.71107362093653	28.138140266810325	32.92598210868055	19.224804003572594
28	18.717836029317947	29.576817840282406	32.54292073663467	19.162425393764977
29	19.964274068928365	29.03582516976906	31.52643293590598	19.4734678253966
30	18.76830599543502	30.21052780810071	33.32407105490736	17.69709514155691
31	19.987240738902987	28.693309894098135	31.940116534584682	19.379332832414196
32	17.493514042275685	29.926421594340557	32.043608319038235	20.53645604434552
33	18.497526121042856	29.789755731034777	32.95943972666827	18.753278421254095
34	17.494081120546664	29.82945121000326	32.21628365255114	20.460184016898932
35	18.743071012376483	30.430270638104822	31.891064264145058	18.935594085373634
36	18.800912996016276	30.412974750839982	31.327672001928065	19.458440251215674
37	18.984079277542282	30.87571061995832	30.749819243801124	19.39039085869827
38	18.659994045678154	30.20939365155876	31.028821753122475	20.101790549640615
39	18.60356975771581	29.374370897543134	31.97243999603045	20.049619348710607
40	18.001332633936798	30.389724541729873	32.096063059103734	19.512879765229595
41	17.972411642116903	29.467938812254562	32.27214086224251	20.287508683386022
42	17.418943249642034	30.302961566270188	32.07990132838085	20.198193855706933
43	18.20122772445667	30.484710152118744	31.03647730978068	20.277584813643905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	99.0
1	116.0
2	133.0
3	434.0
4	735.0
5	735.0
6	1083.0
7	1431.0
8	1483.0
9	1535.0
10	2120.0
11	2705.0
12	2705.0
13	5175.5
14	7646.0
15	11698.5
16	15751.0
17	13375.0
18	10999.0
19	10999.0
20	13708.5
21	16418.0
22	11390.0
23	6362.0
24	6332.0
25	6302.0
26	6302.0
27	6895.0
28	7488.0
29	7588.0
30	7688.0
31	8433.5
32	9179.0
33	9179.0
34	10779.0
35	12379.0
36	13021.5
37	13664.0
38	14366.5
39	15069.0
40	15069.0
41	16527.0
42	17985.0
43	19952.5
44	21920.0
45	24478.5
46	27037.0
47	27037.0
48	29797.0
49	32557.0
50	28696.0
51	24835.0
52	22910.0
53	20985.0
54	20985.0
55	20359.0
56	19733.0
57	18027.5
58	16322.0
59	13666.5
60	11011.0
61	11011.0
62	9375.0
63	7739.0
64	6626.0
65	5513.0
66	4859.0
67	4205.0
68	4205.0
69	3751.0
70	3297.0
71	2536.0
72	1775.0
73	1374.0
74	973.0
75	973.0
76	775.5
77	578.0
78	427.0
79	276.0
80	220.5
81	165.0
82	165.0
83	128.5
84	92.0
85	74.5
86	57.0
87	43.0
88	29.0
89	29.0
90	22.0
91	15.0
92	9.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	352685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.11875313652917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.90579642419897	20.355696288887135
2	12.734205337788085	7.416083632418466
3	5.175463020647717	4.5210909019642935
4	2.736133442680607	3.186911770640731
5	1.691406493347603	2.4625824066655655
6	1.1382399549560787	1.9886476955100083
7	0.8556756876617363	1.7441346379766354
8	0.6220371919867586	1.4490357948161776
9	0.5171994546552936	1.3554182918209507
>10	3.8720067206540087	22.622867421529342
>50	0.49423499914272134	9.872283017474384
>100	0.23463681777482734	12.60085458417827
>500	0.01797218178700805	3.225151240218028
>1k	0.0029953636311680085	2.3767791750457383
>5k	0.0019969090874453386	4.82246314085427
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9480	2.6879510044373873	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7107	2.0151126359215734	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4463	1.265435161688192	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2507	0.710832612671364	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1205	0.34166465826445697	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	911	0.25830415243063926	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	897	0.25433460453379075	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	713	0.20216340360378243	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	659	0.1868522902873669	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	657	0.18628521201638856	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	617	0.17494364659682152	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	609	0.17267533351290812	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	593	0.1681387073450813	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	584	0.16558685512567872	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	579	0.16416915944823285	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	576	0.1633185420417653	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	570	0.16161730722883025	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	560	0.1587819158739385	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	529	0.14999220267377403	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	516	0.14630619391241476	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	513	0.14545557650594723	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	509	0.14432141996399053	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	501	0.1420531068800771	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	494	0.14006833293165288	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	449	0.12730907183463996	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	444	0.1258913761571941	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	416	0.11795228036349717	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	395	0.11199795851822447	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	393	0.11143088024724614	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	390	0.1105802628407786	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	389	0.11029672370528942	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	386	0.1094461062988219	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	368	0.10434240186001673	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	361	0.1023576279115925	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	355	0.10065639309865743	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.835391354891759E-4	0.0
8	0.0	0.0	0.0	2.835391354891759E-4	0.0
9	0.0	0.0	0.0	2.835391354891759E-4	0.0
10	0.0	0.0	0.0	2.835391354891759E-4	0.0
11	0.0	0.0	0.0	2.835391354891759E-4	0.0
12	0.0	0.0	0.0	2.835391354891759E-4	0.0
13	0.0	0.0	0.0	8.506174064675277E-4	0.0
14	0.0	0.0	0.0	8.506174064675277E-4	0.0
15	0.0	0.0	0.0	0.002268313083913407	0.0
16	0.0	0.0	0.0	0.00482016530331599	0.0
17	0.0	0.0	0.0	0.0082226349291861	0.0
18	0.0	0.0	0.0	0.008789713200164452	0.0
19	0.0	0.0	0.0	0.010774487148588684	0.0
20	0.0	0.0	0.0	0.01247572196152374	0.0
21	0.0	0.0	0.0	0.014744035045437146	0.0
22	0.0	0.0	0.0	0.019564200348753138	0.0
23	0.0	0.0	0.0	0.024667904787558304	0.0
24	0.0	0.0	0.0	0.04082963551044133	0.0
25	0.0	0.0	0.0	0.04451564427180062	0.0
26	0.0	0.0	0.0	0.055573670555878474	0.0
27	0.0	0.0	0.0	0.06776585338191304	0.0
28	0.0	0.0	0.0	0.09044898422104711	0.0
29	0.0	0.0	0.0	0.12447368047974822	0.0
30	0.0	0.0	0.0	0.19649262089399888	0.0
31	0.0	0.0	0.0	0.37909182414902814	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	25	0.0054912437	29.599998	3
GATCGCA	25	0.0054912437	29.599998	10
TGATCAC	35	8.8571565E-4	26.428572	21
TTGATCA	35	8.8571565E-4	26.428572	20
ACGTTTA	35	8.8571565E-4	26.428572	26
TCACGTT	35	8.8571565E-4	26.428572	24
GTGGTTT	40	0.0019285484	23.125	37
TATTGTA	45	0.0038206296	20.555555	31
TCAAGAC	55	5.1333837E-4	20.181818	3
TTGTAAG	50	0.0070254533	18.499998	33
TATCTGT	50	0.0070254533	18.499998	33
TCTATGT	50	0.0070254533	18.499998	29
CAGTCGG	85	2.7144575E-5	17.411764	10
TGGACAA	75	2.0621512E-4	17.266666	5
TCTTATA	225	0.0	17.266666	37
AGTTTTT	65	0.0015769998	17.076923	15
GGGTTAG	65	0.0015769998	17.076923	7
GTACTGG	235	0.0	16.531916	1
CTAGAAA	90	4.4319117E-5	16.444445	29
GCAGTCG	90	4.4319117E-5	16.444445	9
>>END_MODULE