FastQCFastQC Report
Fri 10 Feb 2017
ERR1630433.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630433.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410011
Sequences flagged as poor quality0
Sequence length43
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT208335.081083190450988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146553.5742943482004144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122002.9755299248068954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57991.4143522978651792No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA29650.723151330086266No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18670.4553536368536453No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA16360.39901368499869516No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA15100.36828280216872233No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12410.302674806285685No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12410.302674806285685No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10640.25950523278643745No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA10210.2490177092809705No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.21316501264600218No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA8030.19584840406720794No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA7790.18999490257578455No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.17706842011555787No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6530.1592640197458117No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6510.15877622795485977No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.1582884361639078No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6160.15023987161320063No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA6140.1497520798222487No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5920.14438637012177724No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA5860.14292299474892137No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5560.1356061178846421No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5090.1241430107972713No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA4680.11414327908275632No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4660.11365548729180437No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA4430.10804588169585694No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4150.10121679662252964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG303.5954753E-430.8333349
TATGACA250.00549227229.59999819
GTACTGG950.027.2631591
TTCACTA905.456968E-1226.7222218
CATATAG358.859626E-426.428573
GACCGTC358.859626E-426.4285722
TCGTGGT358.859626E-426.4285735
GTCACTC358.859626E-426.428579
CAATGCG358.859626E-426.4285719
TACGTGC358.859626E-426.428578
TATAGTC509.072477E-625.8999985
ATTGACT659.361065E-825.61538531
ACTATCG1002.0008883E-1124.0511
TCACTAT1002.0008883E-1124.059
ATCGGTC1002.0008883E-1124.0514
TCGCCTA551.8987916E-523.54545435
TATCGCC551.8987916E-523.54545433
TCTATCG551.8987916E-523.54545431
AGTCACT400.001929080723.1258
CGACGAG400.001929080723.12524
Produced by FastQC (version 0.11.5)