FastQCFastQC Report
Fri 10 Feb 2017
ERR1630432.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630432.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652182
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG14400.2207972621139498No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG11330.17372451248271187No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT9280.14229156891787875No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA8670.13293835156444062No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC7650.11729854549803581No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG7090.10871198530471556No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC6700.10273205945579608No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC6660.10211873372770178No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6630.10165873943163105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACAC454.0047616E-628.7777773
GTACACG451.3223548E-424.6666664
TAACACC451.3223548E-424.6666664
CATAATA451.3223548E-424.6666662
GTATAGG451.3223548E-424.6666661
ATACCGT451.3223548E-424.6666666
TAGCGTG400.001930296823.1255
CTTATAC3100.020.88709637
TTATACT450.0038240620.5555554
TCGCCAT2350.020.46808613
TATTAGA759.255697E-619.7333342
TTGGCCG2350.019.68085131
CCGCAGA2500.019.2435
GGTATCA4200.018.9404751
TCCATTA609.230974E-418.58
GGGTATT500.007031697318.535
TGTTGAA2600.018.520
TATACTG500.007031697318.55
GCGTACA500.007031697318.52
CGCAGAC2550.018.13725536