FastQCFastQC Report
Fri 10 Feb 2017
ERR1630431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763446
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54010.30627532683166936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47480.26924555671112127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38890.22053411332130388No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG34910.1979646669078611No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA25680.14562396580331918No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA24710.14012337207943992No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT24700.14006666492764733No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC23990.1360404571503749No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG23290.132070956524895No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC20560.11658990408552346No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA18800.10660944537003117No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC17740.10059848728001877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG200.001842202537.05
CGTAATA400.001931594123.1252
GGTATCA15350.020.9706841
TCGTAGA555.144593E-420.1818182
AAGACGG2950.018.8135595
AGTACCG902.1535434E-618.55
CGTATTC500.007036337618.4999983
GCGCAAG3150.018.206351
GACGGAC3050.018.1967227
AGCGAAA2900.017.86206817
TCTATAC2100.017.6190473
GCGAAAG2850.017.52631818
GAGCGAA2900.017.22413816
CGGACCA2900.017.2241389
ATCTCGC5950.017.10084211
TCGCCTA650.001580386217.07692335
ACCGGGC650.001580386217.0769238
ACTTACG650.001580386217.07692316
TCGCCAT5550.017.013
CGCAAGA3050.016.9836062
Produced by FastQC (version 0.11.5)