##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630431.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1763446
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217160604861164	31.0	31.0	34.0	28.0	34.0
2	31.43328970663122	31.0	31.0	34.0	28.0	34.0
3	31.563094078298967	31.0	31.0	34.0	28.0	34.0
4	35.343193950934705	37.0	35.0	37.0	33.0	37.0
5	35.1050488645527	37.0	35.0	37.0	32.0	37.0
6	35.18634820686315	37.0	35.0	37.0	32.0	37.0
7	35.180659912466844	37.0	35.0	37.0	32.0	37.0
8	35.1412325639685	37.0	35.0	37.0	32.0	37.0
9	36.76221727231795	38.0	37.0	39.0	33.0	39.0
10	36.57220805173507	38.0	35.0	39.0	32.0	39.0
11	36.70731057259479	38.0	35.0	39.0	32.0	39.0
12	36.55280569974924	38.0	35.0	39.0	32.0	39.0
13	36.61694205549816	38.0	35.0	39.0	32.0	39.0
14	37.66520551238881	39.0	37.0	40.0	33.0	41.0
15	37.646949211940715	39.0	37.0	40.0	33.0	41.0
16	37.60562841164402	39.0	37.0	40.0	32.0	41.0
17	37.56020087941451	39.0	37.0	40.0	32.0	41.0
18	37.51855117763742	39.0	36.0	40.0	32.0	41.0
19	37.54413404209712	39.0	37.0	40.0	32.0	41.0
20	37.48332356080084	39.0	36.0	40.0	32.0	41.0
21	37.438458563517116	39.0	36.0	40.0	32.0	41.0
22	37.415563050980865	39.0	36.0	40.0	32.0	41.0
23	37.355435323792165	39.0	36.0	40.0	32.0	41.0
24	37.336114630104916	39.0	36.0	40.0	32.0	41.0
25	37.297085365812165	39.0	36.0	40.0	32.0	41.0
26	37.10802599002181	39.0	36.0	40.0	31.0	41.0
27	36.99742662945165	39.0	36.0	40.0	31.0	41.0
28	36.91036187101845	39.0	36.0	40.0	31.0	41.0
29	36.832616932982354	39.0	36.0	40.0	31.0	41.0
30	36.77258447380867	39.0	36.0	40.0	30.0	41.0
31	36.641855775566704	39.0	36.0	40.0	30.0	41.0
32	36.52621288091611	38.0	35.0	40.0	30.0	41.0
33	36.471821082131235	38.0	35.0	40.0	30.0	41.0
34	36.3577648535878	38.0	35.0	40.0	30.0	41.0
35	36.280364695034606	38.0	35.0	40.0	30.0	41.0
36	36.17107980624301	38.0	35.0	40.0	30.0	41.0
37	36.11563552272086	38.0	35.0	40.0	30.0	41.0
38	35.98577501097284	38.0	35.0	40.0	29.0	41.0
39	35.880693256272096	38.0	35.0	40.0	29.0	41.0
40	35.78566227715507	38.0	35.0	40.0	28.0	41.0
41	35.65598379536431	38.0	34.0	40.0	28.0	41.0
42	35.61492781746648	38.0	34.0	40.0	27.0	41.0
43	34.40426244977164	37.0	33.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	4.0
12	3.0
13	5.0
14	7.0
15	16.0
16	43.0
17	84.0
18	135.0
19	306.0
20	572.0
21	1056.0
22	1798.0
23	3106.0
24	5065.0
25	7793.0
26	11354.0
27	16227.0
28	23166.0
29	31340.0
30	40323.0
31	52585.0
32	66602.0
33	85705.0
34	112014.0
35	147944.0
36	209850.0
37	312453.0
38	437026.0
39	196859.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06539128501808	20.505419502496817	13.33769222306779	25.091496989417312
2	17.561014059971217	22.05499913238058	35.99690605779819	24.38708074985001
3	19.407795872399834	23.664121271646536	31.655122980800094	25.272959875153532
4	14.840204916963717	16.264291619930525	35.37800420313409	33.517499259971665
5	13.365478727446147	38.13969920258403	35.06860998295383	13.42621208701599
6	33.537573591706234	36.478633312276074	15.936751111176639	14.047041984841044
7	28.041913390032924	32.36946297193109	21.24590149060419	18.34272214743179
8	26.233635733671456	35.96123725932067	20.035827578502545	17.769299428505324
9	25.872581298208168	15.121132146944108	19.92445473238194	39.08183182246579
10	15.992891191451283	28.40415867568386	33.74614249599931	21.856807636865547
11	34.65867398264535	22.67492171577695	22.04649305961169	20.61991124196601
12	20.311594457669813	26.607789521198832	30.0782672109041	23.00234881022725
13	29.214957532014026	20.320100530438697	26.17885662503984	24.286085312507446
14	21.85380215782054	21.542593308782916	25.922143348874872	30.68146118452167
15	24.322037646743933	28.933633351971082	23.167309914791833	23.577019086493152
16	23.933196706902283	28.270840161819528	24.25977319407569	23.5361899372025
17	22.718302686898266	27.960425212907005	26.103833063218268	23.217439036976465
18	21.847734492578734	26.71139348752386	27.910976576543884	23.529895443353524
19	23.412851882053662	26.74916045061771	27.95963131278191	21.87835635454672
20	23.721905859323165	25.929628692911493	27.926230800376082	22.422234647389256
21	23.221635366209114	27.002924954889462	26.479631358147625	23.295808320753796
22	23.351551450965893	26.503334947596922	26.817719397134926	23.32739420430226
23	22.72102463018431	26.963626898697207	26.984211594797912	23.331136876320567
24	23.978165478273787	26.708217887023476	26.8759576420259	22.437658992676837
25	23.609342163014915	26.063627692597336	27.18489820499182	23.14213193939593
26	23.303407079094	26.707594108353756	26.86745156925701	23.12154724329523
27	23.51957474172728	26.238852791636376	26.707991058416304	23.53358140822004
28	22.701630784271252	26.705269115130264	27.39766343851754	23.195436662080947
29	22.798600013836545	27.163236073007056	27.11151915057223	22.926644762584168
30	22.54789769576159	27.85801209676962	26.96963785678722	22.624452350681562
31	23.352628886849953	26.832803499511755	27.08191801733651	22.732649596301787
32	22.394051192948353	27.05231688410079	27.094563712186254	23.459068210764606
33	22.72936058149782	26.573084744301784	27.41943898480589	23.278115689394514
34	22.939857528951837	26.37642434188515	27.412917662349738	23.270800466813274
35	23.360114230886573	26.518986121491672	27.206276801217616	22.914622846404143
36	22.846007192735133	27.04772360480559	27.15331232144336	22.952956881015922
37	23.835093334301135	25.873545319788644	26.9003417172967	23.39101962861352
38	23.043631616732238	26.50860871271363	27.763367860427824	22.684391810126307
39	23.05599377582302	26.1478378130093	27.75576910208762	23.040399309080062
40	22.69062959682349	25.616321679257542	28.477821265862406	23.215227458056557
41	22.120892842763542	26.337409821451864	28.57756914586554	22.964128189919055
42	22.950064816274498	25.437127079593026	28.179258111674528	23.43354999245795
43	22.232152274580567	25.461397740560244	28.33792472239014	23.96852526246905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	236.0
1	213.5
2	191.0
3	405.0
4	619.0
5	619.0
6	919.5
7	1220.0
8	1209.5
9	1199.0
10	1821.5
11	2444.0
12	2444.0
13	4054.5
14	5665.0
15	9879.5
16	14094.0
17	14154.5
18	14215.0
19	14215.0
20	16511.5
21	18808.0
22	21159.5
23	23511.0
24	26748.0
25	29985.0
26	29985.0
27	34190.5
28	38396.0
29	49125.0
30	59854.0
31	63024.0
32	66194.0
33	66194.0
34	71729.0
35	77264.0
36	81744.5
37	86225.0
38	95702.5
39	105180.0
40	105180.0
41	112611.0
42	120042.0
43	112810.5
44	105579.0
45	111240.0
46	116901.0
47	116901.0
48	120052.0
49	123203.0
50	125843.0
51	128483.0
52	135034.5
53	141586.0
54	141586.0
55	125281.0
56	108976.0
57	101867.5
58	94759.0
59	84473.5
60	74188.0
61	74188.0
62	67879.5
63	61571.0
64	53759.0
65	45947.0
66	39669.0
67	33391.0
68	33391.0
69	28428.5
70	23466.0
71	20080.0
72	16694.0
73	13551.5
74	10409.0
75	10409.0
76	7984.5
77	5560.0
78	4616.0
79	3672.0
80	2671.5
81	1671.0
82	1671.0
83	1235.0
84	799.0
85	665.0
86	531.0
87	433.0
88	335.0
89	335.0
90	312.5
91	290.0
92	173.0
93	56.0
94	40.5
95	25.0
96	25.0
97	16.0
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1763446.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.1852015756797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.37766785218469	46.56398608908561
2	9.728423107868641	10.737299804424627
3	2.504308879286816	4.146023709336224
4	1.0237550694011281	2.259845194761009
5	0.5285115085276093	1.4583007066581342
6	0.33554606690704236	1.1110306404114982
7	0.2300871443954884	0.8888183810406286
8	0.16914223340264728	0.7467318596228604
9	0.12222041310460639	0.6070282320456494
>10	0.7784352990353185	8.206463108193871
>50	0.08954564730684421	3.535789285448634
>100	0.09779616259810028	11.685369507282582
>500	0.010843011900757598	4.118407389783734
>1k	0.0036143373002525325	3.6271140081026907
>5k	1.0326678000721523E-4	0.30779208380226303
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5401	0.30627532683166936	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4748	0.26924555671112127	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3889	0.22053411332130388	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3491	0.1979646669078611	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2568	0.14562396580331918	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2471	0.14012337207943992	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2470	0.14006666492764733	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2399	0.1360404571503749	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2329	0.132070956524895	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2056	0.11658990408552346	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1880	0.10660944537003117	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1774	0.10059848728001877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.6707151792569776E-5	0.0
3	0.0	0.0	0.0	5.6707151792569776E-5	0.0
4	0.0	0.0	0.0	5.6707151792569776E-5	0.0
5	0.0	5.6707151792569776E-5	0.0	5.6707151792569776E-5	0.0
6	0.0	5.6707151792569776E-5	0.0	5.6707151792569776E-5	0.0
7	0.0	5.6707151792569776E-5	0.0	5.6707151792569776E-5	0.0
8	0.0	5.6707151792569776E-5	0.0	5.6707151792569776E-5	0.0
9	0.0	5.6707151792569776E-5	0.0	5.6707151792569776E-5	0.0
10	0.0	5.6707151792569776E-5	0.0	5.6707151792569776E-5	0.0
11	0.0	5.6707151792569776E-5	0.0	5.6707151792569776E-5	0.0
12	0.0	5.6707151792569776E-5	0.0	5.6707151792569776E-5	0.0
13	0.0	5.6707151792569776E-5	0.0	5.6707151792569776E-5	0.0
14	0.0	5.6707151792569776E-5	0.0	5.6707151792569776E-5	0.0
15	0.0	5.6707151792569776E-5	0.0	1.1341430358513955E-4	0.0
16	0.0	5.6707151792569776E-5	0.0	2.268286071702791E-4	0.0
17	0.0	5.6707151792569776E-5	0.0	3.4024291075541866E-4	0.0
18	0.0	5.6707151792569776E-5	0.0	3.969500625479884E-4	0.0
19	0.0	5.6707151792569776E-5	0.0	4.536572143405582E-4	0.0
20	0.0	5.6707151792569776E-5	0.0	4.536572143405582E-4	0.0
21	0.0	5.6707151792569776E-5	0.0	7.371929733034071E-4	0.0
22	0.0	5.6707151792569776E-5	0.0	0.001020728732266256	0.0
23	0.0	5.6707151792569776E-5	0.0	0.0015878002501919536	0.0
24	0.0	5.6707151792569776E-5	0.0	0.0024384075270805004	0.0
25	0.0	5.6707151792569776E-5	0.0	0.003005479045006198	0.0
26	0.0	5.6707151792569776E-5	0.0	0.004196329232650163	0.0
27	0.0	5.6707151792569776E-5	0.0	0.00992375156369971	0.0
28	0.0	5.6707151792569776E-5	0.0	0.026028582672789527	0.0
29	0.0	5.6707151792569776E-5	0.0	0.04899497914878029	0.0
30	0.0	5.6707151792569776E-5	0.0	0.08568450635857293	0.0
31	0.0	5.6707151792569776E-5	0.0	0.20669756828391683	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	20	0.0018422025	37.0	5
CGTAATA	40	0.0019315941	23.125	2
GGTATCA	1535	0.0	20.970684	1
TCGTAGA	55	5.144593E-4	20.181818	2
AAGACGG	295	0.0	18.813559	5
AGTACCG	90	2.1535434E-6	18.5	5
CGTATTC	50	0.0070363376	18.499998	3
GCGCAAG	315	0.0	18.20635	1
GACGGAC	305	0.0	18.196722	7
AGCGAAA	290	0.0	17.862068	17
TCTATAC	210	0.0	17.619047	3
GCGAAAG	285	0.0	17.526318	18
GAGCGAA	290	0.0	17.224138	16
CGGACCA	290	0.0	17.224138	9
ATCTCGC	595	0.0	17.100842	11
TCGCCTA	65	0.0015803862	17.076923	35
ACCGGGC	65	0.0015803862	17.076923	8
ACTTACG	65	0.0015803862	17.076923	16
TCGCCAT	555	0.0	17.0	13
CGCAAGA	305	0.0	16.983606	2
>>END_MODULE