FastQCFastQC Report
Fri 10 Feb 2017
ERR1630430.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630430.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444186
Sequences flagged as poor quality0
Sequence length43
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230455.188141904517477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT159083.5813825739667617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT147033.3100998230471017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99852.2479321725583428No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83601.882094437915648No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83101.8708378922343343No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38740.8721571593881842No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30420.6848482392511245No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30040.676293264533326No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29920.6735916935698109No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25770.5801623644149072No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA18570.41806810660399024No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA18150.40861260823168677No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA16460.37056548382884646No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.28118851111921583No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12370.2784869401557005No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10590.23841363753022382No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA8810.1983403349047471No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.17380106531948328No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA7080.15939268684740177No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6840.1539895449203712No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6590.14836127207971436No Hit
GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6540.14723561751158298No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA6520.14678535568433043No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA6450.14520943928894653No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6340.1427329992390575No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT6330.14250786832543125No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA6170.13890577370741086No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.13530367908939048No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA5920.13327750086675402No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA5760.12967540624873364No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5740.1292251444214811No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5650.12719896619884463No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC5440.12247121701269288No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA5240.11796859874016741No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC5020.1130157186403894No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4800.10806283854061137No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.1060366603179749No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4520.10175917295907569No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA4490.10108378021819688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACTA200.001840720537.05
AAACGAT200.001840720537.028
GTCGTAG200.001840720537.011
AACGATG200.001840720537.029
CGTCGTA200.001840720537.010
ATAAACG200.001840720537.026
ATACCGT200.001840720537.06
TACCGTC200.001840720537.07
ACGATGC251.2324065E-436.99999630
GCAGCGT401.5971418E-632.3751
GCGTTAT303.5959546E-430.8333321
CTTAAGC250.005492758529.5999982
CGACCGG250.005492758529.59999837
CGACCAT250.005492758529.59999821
TTCCGAC250.005492758529.59999818
CGTAGTT250.005492758529.59999813
AGTTGAG250.005492758529.59999826
GATGCCG250.005492758529.59999832
TGGATTT250.005492758529.59999826
ACACTTA250.005492758529.5999986
Produced by FastQC (version 0.11.5)