##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630430.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444186
Sequences flagged as poor quality	0
Sequence length	43
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.620354986424605	31.0	31.0	34.0	30.0	34.0
2	31.665955252979607	31.0	31.0	34.0	30.0	34.0
3	31.69156839702287	31.0	31.0	34.0	28.0	34.0
4	35.44473036070475	37.0	35.0	37.0	33.0	37.0
5	35.4710256514163	37.0	35.0	37.0	33.0	37.0
6	35.624706766985	37.0	35.0	37.0	33.0	37.0
7	35.58350780979139	37.0	35.0	37.0	33.0	37.0
8	35.69085248071754	37.0	35.0	37.0	33.0	37.0
9	37.266609033152776	39.0	37.0	39.0	34.0	39.0
10	37.188785778930445	39.0	37.0	39.0	34.0	39.0
11	37.33142647449492	39.0	37.0	39.0	34.0	39.0
12	37.281436155124204	39.0	37.0	39.0	34.0	39.0
13	37.3101245874476	39.0	37.0	39.0	34.0	39.0
14	38.16217530493982	40.0	37.0	41.0	33.0	41.0
15	38.15473247693534	40.0	37.0	41.0	33.0	41.0
16	38.099717235572484	40.0	37.0	41.0	33.0	41.0
17	38.26016803771393	40.0	37.0	41.0	34.0	41.0
18	38.274013588901944	40.0	37.0	41.0	34.0	41.0
19	38.32491568847285	40.0	38.0	41.0	34.0	41.0
20	38.36265663483316	40.0	38.0	41.0	34.0	41.0
21	38.300169298447045	40.0	37.0	41.0	34.0	41.0
22	38.168931483657744	40.0	37.0	41.0	34.0	41.0
23	37.98326376788102	40.0	36.0	41.0	33.0	41.0
24	37.87732166254677	40.0	36.0	41.0	33.0	41.0
25	37.796677517976704	40.0	36.0	41.0	33.0	41.0
26	37.42938994025025	39.0	35.0	41.0	33.0	41.0
27	37.13519111363258	39.0	35.0	41.0	33.0	41.0
28	36.89860328781186	39.0	35.0	41.0	33.0	41.0
29	36.60206309969247	38.0	35.0	40.0	32.0	41.0
30	36.237378035327545	37.0	35.0	40.0	31.0	41.0
31	35.78935851197471	37.0	35.0	40.0	31.0	41.0
32	35.39659737137145	35.0	35.0	40.0	30.0	41.0
33	35.040417752923325	35.0	35.0	40.0	30.0	41.0
34	34.75612018388693	35.0	34.0	40.0	29.0	41.0
35	34.37500281413642	35.0	34.0	40.0	25.0	41.0
36	33.99816293174481	35.0	33.0	39.0	24.0	41.0
37	33.642624936400516	35.0	33.0	39.0	21.0	41.0
38	33.230880757160286	35.0	33.0	39.0	20.0	41.0
39	32.762029870369616	35.0	33.0	39.0	18.0	41.0
40	32.2538396077319	35.0	33.0	39.0	15.0	41.0
41	31.702791173067137	35.0	32.0	38.0	10.0	41.0
42	31.167713525415028	35.0	31.0	38.0	8.0	41.0
43	30.186991485548848	35.0	27.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	11.0
10	18.0
11	25.0
12	12.0
13	4.0
14	5.0
15	6.0
16	9.0
17	28.0
18	45.0
19	102.0
20	233.0
21	389.0
22	701.0
23	1144.0
24	1819.0
25	2888.0
26	4008.0
27	5850.0
28	7906.0
29	10027.0
30	12399.0
31	14966.0
32	18391.0
33	23195.0
34	30593.0
35	42061.0
36	61905.0
37	86744.0
38	68659.0
39	50042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.39912559153147	20.27821678305935	16.9951326696474	13.327524955761774
2	16.53113785666365	31.02754251597304	32.341856789723224	20.099462837640086
3	23.08627466871986	31.57123367238049	31.345652496926963	13.996839161972687
4	12.927242191334262	18.304268932384183	30.346746633167186	38.42174224311437
5	11.11111111111111	46.14463310415006	27.003102303989767	15.741153480749057
6	26.412133655720805	33.6140715826253	20.272813641132316	19.700981120521583
7	22.2580180374888	37.56129189123475	22.018028483563192	18.162661587713256
8	33.54428099940115	28.8464742247617	21.337007469843712	16.272237305993436
9	29.387463810205634	12.01433633657972	24.826086369223706	33.77211348399094
10	20.01909110147551	34.58100885665014	26.312850922811613	19.087049119062733
11	27.675568342991454	28.385631244568714	19.690625098494774	24.248175313945058
12	19.894818837153807	37.50838612653258	28.41512339425376	14.181671642059857
13	31.252673429599316	22.810939561354928	30.06983560940687	15.86655139963889
14	21.707798084586187	25.991589109066922	35.66726551489691	16.63334729144998
15	23.33954694654942	29.930704704785832	31.333495427591146	15.396252921073605
16	15.87623202892482	29.581301526837855	33.468862143336345	21.073604300900975
17	14.96805392335643	32.68810813488044	34.17262137933208	18.171216562431052
18	13.715650650853473	26.68093996659057	39.97852251084005	19.6248868717159
19	15.273781704060912	29.65176750280288	42.56009869739253	12.514352095743675
20	16.939750464895337	27.49658926665856	43.05110922001143	12.512551048434664
21	18.969305651236194	27.693353685167928	40.310815739352435	13.026524924243448
22	17.92537360475116	29.22514442148109	40.02219790808355	12.827284065684195
23	15.653352424434807	30.374437735543218	39.94812983750051	14.024080002521465
24	13.671975253609975	32.348385586218384	40.997014764085314	12.982624396086322
25	13.2077553097126	32.18584106658022	40.34233406726011	14.264069556447073
26	13.917818211289864	32.638354202969026	40.21310892283863	13.230718662902477
27	13.21473436803501	33.58728100390377	40.2831246369764	12.914859991084814
28	12.947053711733373	33.362150090277495	40.93555402466534	12.755242173323788
29	12.490713349812916	33.59380980039893	41.730491280679715	12.184985569108436
30	12.007132147343679	35.15509268639714	40.91844407522975	11.919331091029434
31	12.721022274452595	35.454516801520086	39.5179046615607	12.306556262466623
32	10.906692241538455	35.897349308622964	40.380606322576575	12.815352127262003
33	11.126420013237698	35.48198277298249	40.778187516040575	12.613409697739236
34	11.483477642248967	34.90429684861747	40.44994664397347	13.16227886516009
35	11.174598028753719	35.82508228534893	39.68630258495315	13.3140171009442
36	11.061582310113332	36.10086765454111	38.84026061154561	13.997289423799938
37	11.0338912077373	36.132385982448795	39.085203045571	13.748519764242909
38	11.392975014971206	34.94459528215658	39.11874755170132	14.543682151170906
39	11.286488092825978	35.026092672889284	39.696208345152705	13.99121088913203
40	11.12979697694209	35.07697225936882	39.57643869910353	14.216792064585556
41	10.989540417752924	35.19944347638152	39.54897272764112	14.262043378224437
42	10.699796931915909	35.03982565862049	39.487962250048405	14.7724151594152
43	9.8454251147042	34.902946063135715	39.01811403331037	16.233514788849714
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17467.0
1	10517.5
2	3568.0
3	4389.5
4	5211.0
5	5211.0
6	6170.5
7	7130.0
8	6075.5
9	5021.0
10	7566.5
11	10112.0
12	10112.0
13	18490.0
14	26868.0
15	37863.5
16	48859.0
17	40303.0
18	31747.0
19	31747.0
20	31556.5
21	31366.0
22	22449.0
23	13532.0
24	13172.0
25	12812.0
26	12812.0
27	11976.0
28	11140.0
29	10473.5
30	9807.0
31	9262.5
32	8718.0
33	8718.0
34	8472.0
35	8226.0
36	8047.5
37	7869.0
38	8514.5
39	9160.0
40	9160.0
41	10461.0
42	11762.0
43	12549.0
44	13336.0
45	15157.5
46	16979.0
47	16979.0
48	18211.5
49	19444.0
50	17238.5
51	15033.0
52	14932.5
53	14832.0
54	14832.0
55	15601.0
56	16370.0
57	17424.0
58	18478.0
59	17589.0
60	16700.0
61	16700.0
62	13932.0
63	11164.0
64	9561.0
65	7958.0
66	6749.5
67	5541.0
68	5541.0
69	4361.5
70	3182.0
71	2556.0
72	1930.0
73	1467.0
74	1004.0
75	1004.0
76	786.0
77	568.0
78	470.0
79	372.0
80	360.0
81	348.0
82	348.0
83	286.5
84	225.0
85	175.0
86	125.0
87	114.0
88	103.0
89	103.0
90	95.0
91	87.0
92	54.0
93	21.0
94	14.5
95	8.0
96	8.0
97	5.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	444186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.70920583659701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.43828418152646	30.332638153438662
2	10.261430199135404	7.73900767114139
3	3.4241102592154466	3.8736143571587647
4	1.6492565861451691	2.4876862433724587
5	0.9472119093627006	1.7859304430517073
6	0.549020833345174	1.242188376791591
7	0.38184543790587927	1.0079361751030826
8	0.30109885066536934	0.9083358829522556
9	0.22645491225963865	0.7685491409196505
>10	1.5304313953499242	11.191943545184161
>50	0.1573970770131035	4.146147396857287
>100	0.11191351103213062	8.183969584129798
>500	0.011370891495243217	2.711966757458851
>1k	0.006583147707772389	5.495698966267616
>5k	0.0017954039203015608	6.015434294754571
>10k+	0.0017954039203015608	12.108953011418167
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23045	5.188141904517477	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15908	3.5813825739667617	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14703	3.3100998230471017	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9985	2.2479321725583428	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8360	1.882094437915648	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8310	1.8708378922343343	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3874	0.8721571593881842	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3042	0.6848482392511245	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3004	0.676293264533326	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2992	0.6735916935698109	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2577	0.5801623644149072	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1857	0.41806810660399024	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1815	0.40861260823168677	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1646	0.37056548382884646	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1249	0.28118851111921583	No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1237	0.2784869401557005	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1059	0.23841363753022382	No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	881	0.1983403349047471	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	772	0.17380106531948328	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	708	0.15939268684740177	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	684	0.1539895449203712	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	659	0.14836127207971436	No Hit
GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	654	0.14723561751158298	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	652	0.14678535568433043	No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA	645	0.14520943928894653	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	634	0.1427329992390575	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	633	0.14250786832543125	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	617	0.13890577370741086	No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	601	0.13530367908939048	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	592	0.13327750086675402	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	576	0.12967540624873364	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	574	0.1292251444214811	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	565	0.12719896619884463	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	544	0.12247121701269288	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	524	0.11796859874016741	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	502	0.1130157186403894	No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	480	0.10806283854061137	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	471	0.1060366603179749	No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	452	0.10175917295907569	No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA	449	0.10108378021819688	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.2513091362627368E-4	0.0
10	0.0	0.0	0.0	4.5026182725254737E-4	0.0
11	0.0	0.0	0.0	0.001350785481757642	0.0
12	0.0	0.0	0.0	0.002026178222636463	0.0
13	0.0	0.0	0.0	0.0022513091362627367	0.0
14	0.0	0.0	0.0	0.0033769637043941053	0.0
15	0.0	0.0	0.0	0.004952880099778021	0.0
16	0.0	0.0	0.0	0.009230367458677221	0.0
17	0.0	0.0	0.0	0.01530890212658661	0.0
18	0.0	0.0	0.0	0.01823560400372817	0.0
19	0.0	0.0	0.0	0.02318848410350619	0.0
20	0.0	0.0	0.0	0.027691102376031663	0.0
21	0.0	0.0	0.0	0.038272255316466525	0.0
22	0.0	0.0	0.0	0.05830890662920488	0.0
23	0.0	0.0	0.0	0.08262304530084244	0.0
24	0.0	0.0	0.0	0.11841886056741996	0.0
25	0.0	0.0	0.0	0.1301256680759862	0.0
26	0.0	0.0	0.0	0.1515131048704822	0.0
27	0.0	0.0	0.0	0.19428797845947418	0.0
28	0.0	0.0	0.0	0.29199479497327696	0.0
29	0.0	0.0	0.0	0.44575920898002186	0.0
30	0.0	0.0	0.0	0.7460838477574709	0.0
31	0.0	0.0	0.0	1.3154399283183171	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACTA	20	0.0018407205	37.0	5
AAACGAT	20	0.0018407205	37.0	28
GTCGTAG	20	0.0018407205	37.0	11
AACGATG	20	0.0018407205	37.0	29
CGTCGTA	20	0.0018407205	37.0	10
ATAAACG	20	0.0018407205	37.0	26
ATACCGT	20	0.0018407205	37.0	6
TACCGTC	20	0.0018407205	37.0	7
ACGATGC	25	1.2324065E-4	36.999996	30
GCAGCGT	40	1.5971418E-6	32.375	1
GCGTTAT	30	3.5959546E-4	30.833332	1
CTTAAGC	25	0.0054927585	29.599998	2
CGACCGG	25	0.0054927585	29.599998	37
CGACCAT	25	0.0054927585	29.599998	21
TTCCGAC	25	0.0054927585	29.599998	18
CGTAGTT	25	0.0054927585	29.599998	13
AGTTGAG	25	0.0054927585	29.599998	26
GATGCCG	25	0.0054927585	29.599998	32
TGGATTT	25	0.0054927585	29.599998	26
ACACTTA	25	0.0054927585	29.599998	6
>>END_MODULE