FastQCFastQC Report
Fri 10 Feb 2017
ERR1630429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905650
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70770.37136934904100966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59100.3101304017002073No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC53700.2817936137275995No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT48820.2561855534856873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48060.2521974129562092No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA41400.2172487077899929No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG36460.19132579434838506No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA36320.19059113688242857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35110.18424159735523313No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG31640.16603258730616852No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG31380.16466822344082072No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG30670.16094246057775563No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA30040.1576365019809514No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG28780.1510245847873429No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA27670.14519980059297355No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA26850.14089680686379974No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG26450.13879778553249547No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG24250.12725316821032193No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT24110.12651851074436546No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC22400.11754519455303965No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC21120.11082832629286596No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG20790.10909663369453992No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA20070.10531839529819222No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG19860.10421640909925747No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA19540.10253719203421406No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA19460.10211738776795319No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC19400.10180253456825755No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC19380.10169758350169232No Hit
GAGTGGGGGTCCCCGCATGCTGCTGTCCCCAGGGAGCTCAGCC19370.10164510796840973No Hit
GGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGCCAGCGT19260.10106787710230106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.023.6861931
GTATCAA35100.016.0227932
GCTTTAT11950.015.9456071
GTAAACG1455.350921E-815.31034527
ATAACGC1701.4879333E-915.2352933
CGAACGA1001.09396366E-414.816
CGACGGT1406.0003185E-714.5357157
AAGACGG2700.014.3888885
TAACGCC1702.4392648E-814.14705854
TTATTGA13600.014.14705854
TACCGTC1458.9180503E-714.0344837
TTTATTG14900.014.0302013
CGCATCG1753.569403E-813.74285813
AACGCCG1652.5974077E-713.4545455
GCCGCAT1805.163747E-813.36111111
CTTTATT16600.013.2620492
GACGGTA1551.8891751E-613.1290328
GACGGAC2551.6370905E-1113.0588247
TGAACCG850.00940770113.0588235
AAACGCT1703.734549E-713.05882329
Produced by FastQC (version 0.11.5)