##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630428.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4770
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.740041928721173	31.0	30.0	33.0	27.0	34.0
2	31.02389937106918	31.0	30.0	34.0	27.0	34.0
3	31.045702306079665	31.0	31.0	34.0	27.0	34.0
4	34.924528301886795	35.0	35.0	37.0	32.0	37.0
5	34.727253668763105	35.0	35.0	37.0	32.0	37.0
6	34.79853249475891	35.0	35.0	37.0	32.0	37.0
7	34.689727463312366	35.0	35.0	37.0	31.0	37.0
8	34.772327044025154	35.0	35.0	37.0	32.0	37.0
9	36.274423480083854	38.0	35.0	39.0	32.0	39.0
10	36.06603773584906	38.0	35.0	39.0	30.0	39.0
11	36.137735849056604	38.0	35.0	39.0	32.0	39.0
12	36.06058700209643	37.0	35.0	39.0	31.0	39.0
13	36.100628930817614	38.0	35.0	39.0	30.0	39.0
14	36.98805031446541	39.0	36.0	40.0	31.0	41.0
15	36.947169811320755	38.0	36.0	40.0	31.0	41.0
16	36.8685534591195	38.0	36.0	40.0	31.0	41.0
17	36.89874213836478	38.0	36.0	40.0	31.0	41.0
18	36.83375262054507	38.0	36.0	40.0	31.0	41.0
19	36.94234800838574	39.0	36.0	40.0	31.0	41.0
20	36.98909853249476	39.0	36.0	40.0	31.0	41.0
21	36.68825995807128	39.0	35.0	40.0	30.0	41.0
22	36.70943396226415	39.0	35.0	40.0	30.0	41.0
23	36.61551362683438	38.0	35.0	40.0	30.0	41.0
24	36.38867924528302	38.0	35.0	40.0	30.0	41.0
25	36.49601677148847	38.0	35.0	40.0	30.0	41.0
26	36.166457023060794	38.0	34.0	40.0	29.0	41.0
27	36.015513626834384	38.0	34.0	40.0	29.0	41.0
28	36.01404612159329	38.0	34.0	40.0	29.0	41.0
29	35.79538784067086	38.0	34.0	40.0	27.0	41.0
30	35.68385744234801	38.0	34.0	40.0	27.0	41.0
31	35.61425576519916	38.0	34.0	40.0	27.0	41.0
32	35.36058700209644	38.0	34.0	40.0	26.0	41.0
33	35.20712788259958	38.0	33.0	40.0	26.0	41.0
34	35.02914046121593	38.0	33.0	40.0	25.0	41.0
35	34.89811320754717	38.0	33.0	40.0	25.0	41.0
36	34.75974842767295	38.0	33.0	40.0	25.0	41.0
37	34.84046121593291	38.0	33.0	40.0	25.0	41.0
38	34.648637316561846	37.0	33.0	40.0	24.0	41.0
39	34.49287211740042	37.0	33.0	40.0	24.0	41.0
40	34.36708595387841	37.0	33.0	40.0	23.0	41.0
41	34.25744234800838	37.0	33.0	40.0	23.0	41.0
42	34.15911949685535	37.0	32.0	40.0	23.0	41.0
43	32.799790356394126	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	3.0
21	7.0
22	11.0
23	13.0
24	34.0
25	30.0
26	72.0
27	77.0
28	110.0
29	147.0
30	187.0
31	198.0
32	257.0
33	308.0
34	348.0
35	405.0
36	519.0
37	560.0
38	779.0
39	702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0125786163522	19.161425576519918	12.767295597484276	26.058700209643604
2	18.88888888888889	20.58700209643606	33.77358490566038	26.750524109014673
3	19.72746331236897	22.80922431865828	30.398322851153043	27.064989517819704
4	16.855345911949684	15.786163522012577	33.270440251572325	34.088050314465406
5	14.654088050314465	37.67295597484277	33.123689727463315	14.549266247379455
6	34.77987421383648	35.094339622641506	15.974842767295597	14.150943396226415
7	27.379454926624735	30.943396226415093	21.844863731656183	19.832285115303982
8	26.666666666666668	32.725366876310275	20.125786163522015	20.48218029350105
9	27.127882599580712	14.08805031446541	19.22431865828092	39.55974842767296
10	16.81341719077568	27.840670859538786	32.075471698113205	23.270440251572328
11	35.22012578616352	21.29979035639413	21.865828092243188	21.61425576519916
12	21.865828092243188	25.59748427672956	27.505241090146747	25.031446540880502
13	29.559748427672954	19.89517819706499	25.366876310272534	25.178197064989515
14	22.955974842767297	20.230607966457022	26.247379454926623	30.566037735849054
15	25.38784067085954	27.81970649895178	22.70440251572327	24.08805031446541
16	25.07337526205451	26.457023060796647	23.039832285115306	25.429769392033542
17	23.354297693920337	27.064989517819704	24.968553459119498	24.61215932914046
18	23.58490566037736	24.88469601677149	26.60377358490566	24.926624737945495
19	25.052410901467503	25.366876310272534	27.10691823899371	22.473794549266245
20	24.21383647798742	25.303983228511527	25.849056603773583	24.633123689727462
21	24.339622641509433	24.59119496855346	26.436058700209642	24.633123689727462
22	25.38784067085954	25.471698113207548	25.660377358490567	23.48008385744235
23	24.10901467505241	24.9895178197065	25.178197064989515	25.72327044025157
24	25.11530398322851	25.57651991614256	26.07966457023061	23.228511530398325
25	25.744234800838573	24.88469601677149	26.247379454926623	23.12368972746331
26	24.90566037735849	25.744234800838573	25.324947589098535	24.0251572327044
27	24.59119496855346	26.645702306079666	24.80083857442348	23.962264150943398
28	24.234800838574426	26.60377358490566	25.828092243186585	23.333333333333332
29	24.82180293501048	26.687631027253666	26.037735849056602	22.452830188679247
30	24.27672955974843	25.07337526205451	26.939203354297693	23.71069182389937
31	24.863731656184488	26.163522012578618	24.69601677148847	24.27672955974843
32	23.89937106918239	25.010482180293504	26.47798742138365	24.61215932914046
33	23.29140461215933	25.010482180293504	27.379454926624735	24.31865828092243
34	24.0251572327044	25.07337526205451	26.771488469601678	24.129979035639412
35	24.77987421383648	24.50733752620545	25.995807127882596	24.71698113207547
36	24.10901467505241	26.39412997903564	25.241090146750523	24.255765199161424
37	24.737945492662476	24.633123689727462	26.28930817610063	24.339622641509433
38	23.752620545073373	25.471698113207548	27.232704402515722	23.542976939203356
39	23.542976939203356	26.058700209643604	27.10691823899371	23.29140461215933
40	23.08176100628931	25.324947589098535	27.29559748427673	24.29769392033543
41	22.452830188679247	25.157232704402517	27.90356394129979	24.48637316561845
42	23.333333333333332	24.88469601677149	27.882599580712785	23.89937106918239
43	21.9916142557652	23.647798742138367	29.60167714884696	24.758909853249474
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	4.0
2	4.0
3	3.0
4	2.0
5	2.0
6	5.0
7	8.0
8	5.5
9	3.0
10	7.0
11	11.0
12	11.0
13	16.0
14	21.0
15	37.0
16	53.0
17	44.5
18	36.0
19	36.0
20	45.0
21	54.0
22	51.0
23	48.0
24	56.5
25	65.0
26	65.0
27	68.5
28	72.0
29	92.0
30	112.0
31	115.0
32	118.0
33	118.0
34	143.5
35	169.0
36	187.0
37	205.0
38	221.5
39	238.0
40	238.0
41	256.5
42	275.0
43	285.0
44	295.0
45	297.0
46	299.0
47	299.0
48	313.0
49	327.0
50	331.0
51	335.0
52	335.0
53	335.0
54	335.0
55	330.5
56	326.0
57	308.5
58	291.0
59	267.0
60	243.0
61	243.0
62	226.0
63	209.0
64	194.0
65	179.0
66	151.5
67	124.0
68	124.0
69	105.0
70	86.0
71	86.5
72	87.0
73	74.0
74	61.0
75	61.0
76	46.0
77	31.0
78	24.0
79	17.0
80	14.5
81	12.0
82	12.0
83	11.5
84	11.0
85	7.0
86	3.0
87	2.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4770.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.0251572327044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.94202898550725	90.20964360587001
2	3.1438127090301005	5.911949685534592
3	0.5797101449275363	1.6352201257861636
4	0.13377926421404682	0.5031446540880503
5	0.06688963210702341	0.3144654088050315
6	0.022296544035674472	0.12578616352201258
7	0.044593088071348944	0.29350104821802936
8	0.0	0.0
9	0.0	0.0
>10	0.06688963210702341	1.0062893081761006
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.3983228511530398	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.37735849056603776	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.23060796645702308	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTC	7	0.14675052410901468	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	7	0.14675052410901468	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	6	0.12578616352201258	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	5	0.10482180293501049	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.10482180293501049	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	5	0.10482180293501049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020964360587002098	0.0
2	0.0	0.0	0.0	0.020964360587002098	0.0
3	0.0	0.0	0.0	0.020964360587002098	0.0
4	0.0	0.0	0.0	0.020964360587002098	0.0
5	0.0	0.0	0.0	0.020964360587002098	0.0
6	0.0	0.0	0.0	0.020964360587002098	0.0
7	0.0	0.0	0.0	0.020964360587002098	0.0
8	0.0	0.0	0.0	0.020964360587002098	0.0
9	0.0	0.0	0.0	0.020964360587002098	0.0
10	0.0	0.0	0.0	0.020964360587002098	0.0
11	0.0	0.0	0.0	0.020964360587002098	0.0
12	0.0	0.0	0.0	0.020964360587002098	0.0
13	0.0	0.0	0.0	0.020964360587002098	0.0
14	0.0	0.0	0.0	0.020964360587002098	0.0
15	0.0	0.0	0.0	0.020964360587002098	0.0
16	0.0	0.0	0.0	0.020964360587002098	0.0
17	0.0	0.0	0.0	0.020964360587002098	0.0
18	0.0	0.0	0.0	0.020964360587002098	0.0
19	0.0	0.0	0.0	0.020964360587002098	0.0
20	0.0	0.0	0.0	0.020964360587002098	0.0
21	0.0	0.0	0.0	0.020964360587002098	0.0
22	0.0	0.0	0.0	0.020964360587002098	0.0
23	0.0	0.0	0.0	0.020964360587002098	0.0
24	0.0	0.0	0.0	0.020964360587002098	0.0
25	0.0	0.0	0.0	0.020964360587002098	0.0
26	0.0	0.0	0.0	0.020964360587002098	0.0
27	0.0	0.0	0.0	0.020964360587002098	0.0
28	0.0	0.0	0.0	0.06289308176100629	0.0
29	0.0	0.0	0.0	0.08385744234800839	0.0
30	0.0	0.0	0.0	0.10482180293501048	0.0
31	0.0	0.0	0.0	0.20964360587002095	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE