FastQCFastQC Report
Fri 10 Feb 2017
ERR1630427.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630427.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165594
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56210.4822433883496312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46790.4014262255982786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30850.26467191835235937No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.19964069821910546No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC23030.19758166222544044No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT22090.1895171045835857No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT15280.1310919582633404No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT11840.10157910902080829No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG11760.10089276368958659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG250.00549637429.635
AATGCGT957.1449904E-921.42105335
ACGGACC2850.018.8245628
CGCAAGA3300.018.5000022
AAGACGG3100.018.55
CGAATTA902.15227E-618.515
CGAGCGT500.00703493918.56
GTATACT902.15227E-618.54
CGGACCA2900.018.59
GACGGAC3100.017.9032257
CGTTTAG953.6064757E-617.52631626
TATTAGA1705.456968E-1217.4117642
TATACTG852.7231692E-517.4117645
CTTATAC4250.016.97646937
GGTATAC1107.80783E-716.8181823
CAAGACG3600.016.4444454
AAATGCG1251.6576632E-716.27999934
GCAAGAC4450.015.7977533
CTAGCCT1659.731593E-1015.6969714
GCATATA957.059814E-515.5789481
Produced by FastQC (version 0.11.5)