FastQCFastQC Report
Fri 10 Feb 2017
ERR1630426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521556
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC16570.10890167696752535No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16240.10673284453546238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7900.023.1835441
AAGACGG3950.020.6075955
CTACCGA701.2195265E-418.54
GTACTAG650.001580252317.0769231
TACCGAG650.001580252317.0769235
CGCAAGA4400.016.8181822
GCGCAAG4650.016.3118271
ACGGACC4650.016.3118278
CGGACCA4650.016.3118279
GACGGAC4600.016.0869567
CGAACGA2650.016.05660416
CAAGACG5200.016.0096154
ACGAACG2700.015.75925915
AGACGGA4750.015.5789476
CTTAGGA4450.015.3820232
GCGAAAG4900.015.10204118
AGCGAAA4900.015.10204117
GCTTAGG4350.014.8850581
CGTCCTA750.004105218714.833
CCAATAC1252.960087E-614.7999993
Produced by FastQC (version 0.11.5)