##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630426.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1521556
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.829358235911133	33.0	31.0	34.0	30.0	34.0
2	32.04731866589202	33.0	31.0	34.0	30.0	34.0
3	32.168183753999195	34.0	31.0	34.0	30.0	34.0
4	35.80686744359064	37.0	35.0	37.0	35.0	37.0
5	35.73404133663171	37.0	35.0	37.0	35.0	37.0
6	35.78915859817187	37.0	35.0	37.0	35.0	37.0
7	35.757926096706264	37.0	35.0	37.0	33.0	37.0
8	35.75853402700919	37.0	35.0	37.0	33.0	37.0
9	37.51120497701038	39.0	37.0	39.0	35.0	39.0
10	37.40421318702696	39.0	37.0	39.0	34.0	39.0
11	37.471343151352954	39.0	37.0	39.0	35.0	39.0
12	37.39019201396465	39.0	37.0	39.0	34.0	39.0
13	37.394344999461076	39.0	37.0	39.0	34.0	39.0
14	38.629281472387476	40.0	38.0	41.0	34.0	41.0
15	38.636348580006256	40.0	38.0	41.0	34.0	41.0
16	38.58991190596994	40.0	38.0	41.0	34.0	41.0
17	38.554783392790014	40.0	38.0	41.0	34.0	41.0
18	38.49421841851368	40.0	38.0	41.0	34.0	41.0
19	38.52297122156529	40.0	38.0	41.0	34.0	41.0
20	38.49594625501789	40.0	38.0	41.0	34.0	41.0
21	38.42840881308345	40.0	38.0	41.0	34.0	41.0
22	38.396425764151964	40.0	38.0	41.0	34.0	41.0
23	38.32926030984071	40.0	38.0	41.0	34.0	41.0
24	38.283712199879595	40.0	38.0	41.0	34.0	41.0
25	38.26448057120474	40.0	38.0	41.0	34.0	41.0
26	38.0985070546204	40.0	38.0	41.0	33.0	41.0
27	37.97900241594788	40.0	37.0	41.0	33.0	41.0
28	37.88604625790967	40.0	37.0	41.0	33.0	41.0
29	37.83164273940624	40.0	37.0	41.0	33.0	41.0
30	37.76416181855942	40.0	37.0	41.0	32.0	41.0
31	37.722216599323325	40.0	37.0	41.0	32.0	41.0
32	37.63036391693766	40.0	37.0	41.0	32.0	41.0
33	37.530708695572166	40.0	37.0	41.0	32.0	41.0
34	37.4815024882423	40.0	37.0	41.0	32.0	41.0
35	37.346645144838575	40.0	37.0	41.0	31.0	41.0
36	37.26350196772252	40.0	36.0	41.0	31.0	41.0
37	37.208663368288775	39.0	36.0	41.0	31.0	41.0
38	37.08637539466178	39.0	36.0	41.0	31.0	41.0
39	37.01187468617652	39.0	36.0	41.0	31.0	41.0
40	36.90533900822579	39.0	36.0	41.0	30.0	41.0
41	36.77713669427875	39.0	35.0	41.0	30.0	41.0
42	36.73871221302404	39.0	35.0	41.0	30.0	41.0
43	35.44671441603201	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	2.0
12	0.0
13	3.0
14	2.0
15	7.0
16	17.0
17	38.0
18	96.0
19	170.0
20	299.0
21	561.0
22	1087.0
23	1757.0
24	2855.0
25	4431.0
26	6401.0
27	9419.0
28	13169.0
29	17972.0
30	24036.0
31	32009.0
32	41012.0
33	52701.0
34	69775.0
35	92160.0
36	128031.0
37	191646.0
38	355310.0
39	476585.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.471719739529796	18.896774091784987	12.078950758302685	26.552555410382528
2	19.802097326684002	20.89814637121473	32.9387810898843	26.36097521221697
3	20.426852511507956	22.430656512149405	29.135306225994967	28.00718475034767
4	15.382608329893872	15.41665242685777	33.46350709405372	35.73723214919464
5	15.551908703984605	35.521400461106914	33.46436148258756	15.462329352320914
6	35.71390077000123	34.0017718703748	15.305319028678538	14.979008330945426
7	29.811587611629147	29.68789844080665	20.891902762698187	19.608611184866017
8	27.426529158308995	32.38408576483547	19.992757414120806	20.196627662734727
9	26.90009437707189	13.930739322114993	19.168206756767415	40.0009595440457
10	17.472048350504352	25.82363054662464	32.448296349263515	24.25602475360749
11	36.86298762582514	21.19488208123789	20.674230853152956	21.26789943978401
12	22.07187905013026	24.93565797118213	28.154993966702506	24.8374690119851
13	30.398289645599636	18.983790277847152	24.59948894421237	26.01843113234084
14	23.575931480668473	20.578867948337095	23.47839974342055	32.366800827573876
15	26.185168340830046	27.195712809781565	21.089923735965023	25.529195113423363
16	26.515882425622195	25.398079334575925	22.78680508637211	25.29923315342978
17	24.910552092726128	25.591828365173548	24.076800328085195	25.42081921401513
18	24.846472952687908	24.096451264363587	25.48594990917193	25.57112587377658
19	25.455060477563755	24.521476698853018	25.0334525972097	24.990010226373528
20	25.75133613222254	23.874573134344054	24.86191766849199	25.51217306494142
21	26.15736785238269	24.63675342872691	24.20265833133976	25.00322038755064
22	25.998780196062455	24.21054499472908	24.420527407469724	25.370147401738745
23	24.98935300442442	24.337125942127663	24.87907116136376	25.794449892084153
24	25.309617260225714	24.933883471919536	24.65338114403939	25.10311812381536
25	25.841704150225166	24.35546243450783	24.436432178638185	25.36640123662882
26	25.559361600887513	25.285431492498468	24.313465951959703	24.841740954654316
27	26.305045624347706	23.99004703080268	24.155666961978394	25.54924038287122
28	25.146034717092242	24.314517507078282	25.131247223237263	25.408200552592213
29	24.623477545354884	24.682496076384965	25.200847027648017	25.493179350612138
30	24.5124070359553	25.596626085402047	25.026551766744042	24.86441511189861
31	25.750876076858166	24.91226086979382	24.249452534116394	25.08741051923163
32	24.826164794460407	24.592982446916185	24.494464876744594	26.086387881878814
33	24.687162352223645	24.318789449747495	24.993099169534343	26.000949028494514
34	25.675689885879983	24.196874778187592	24.864678000678254	25.262757335254175
35	25.761720239018477	24.227041265651742	25.306331150480165	24.704907344849612
36	24.64273414846381	25.207484969334025	24.858631558746442	25.291149323455723
37	25.950934438167245	23.836322816905852	24.82110418545226	25.391638559474643
38	24.807762579885328	24.204432830602357	25.686271159260656	25.301533430251666
39	25.323484643351936	23.868986747776617	25.317635368004858	25.489893240866586
40	24.856857059483843	23.85584230879442	26.111756649114458	25.17554398260728
41	24.108938481396674	24.165788179994692	26.670723916832507	25.05454942177613
42	25.22095801929078	23.742405800378034	26.289469464153804	24.74716671617739
43	24.272192413555597	23.106543564614118	26.598298058040587	26.0229659637897
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	53.5
2	61.0
3	147.5
4	234.0
5	234.0
6	306.0
7	378.0
8	369.5
9	361.0
10	511.0
11	661.0
12	661.0
13	1160.0
14	1659.0
15	2546.5
16	3434.0
17	3610.0
18	3786.0
19	3786.0
20	4843.0
21	5900.0
22	6864.5
23	7829.0
24	9628.5
25	11428.0
26	11428.0
27	13869.5
28	16311.0
29	21538.5
30	26766.0
31	31025.5
32	35285.0
33	35285.0
34	40789.0
35	46293.0
36	52861.5
37	59430.0
38	67836.5
39	76243.0
40	76243.0
41	81898.5
42	87554.0
43	88403.5
44	89253.0
45	95647.0
46	102041.0
47	102041.0
48	106817.5
49	111594.0
50	114521.0
51	117448.0
52	124475.0
53	131502.0
54	131502.0
55	123567.0
56	115632.0
57	110641.0
58	105650.0
59	98406.5
60	91163.0
61	91163.0
62	85023.5
63	78884.0
64	70127.5
65	61371.0
66	53754.0
67	46137.0
68	46137.0
69	38651.5
70	31166.0
71	27401.0
72	23636.0
73	18609.0
74	13582.0
75	13582.0
76	10405.0
77	7228.0
78	6036.5
79	4845.0
80	3875.5
81	2906.0
82	2906.0
83	2125.5
84	1345.0
85	1179.0
86	1013.0
87	899.0
88	785.0
89	785.0
90	673.5
91	562.0
92	329.5
93	97.0
94	69.0
95	41.0
96	41.0
97	26.5
98	12.0
99	8.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1521556.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.01850588215405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.86513962694676	43.10520753109847
2	9.957452718118716	10.359436255774586
3	2.8467270960032076	4.442474705649905
4	1.240875422996365	2.5819394196062726
5	0.7007448194590169	1.8225849256458926
6	0.43224758880903097	1.3490924244605689
7	0.295992597133074	1.0777964858528606
8	0.23053305474012475	0.9593588051224097
9	0.1606776622943296	0.7522390729069511
>10	0.9971117006476371	9.951683152593594
>50	0.1276113131073161	4.706336484384345
>100	0.1320725061349648	13.844212672182877
>500	0.011037710336832715	3.8703354205530514
>1k	0.0017761832725937702	1.1773026441682304
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1657	0.10890167696752535	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1624	0.10673284453546238	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	6.572219491099901E-5	0.0	0.0	0.0	0.0
12	6.572219491099901E-5	0.0	0.0	0.0	0.0
13	6.572219491099901E-5	0.0	0.0	0.0	0.0
14	6.572219491099901E-5	0.0	0.0	0.0	0.0
15	6.572219491099901E-5	6.572219491099901E-5	0.0	0.0	0.0
16	6.572219491099901E-5	6.572219491099901E-5	0.0	6.572219491099901E-5	0.0
17	6.572219491099901E-5	6.572219491099901E-5	0.0	1.3144438982199802E-4	0.0
18	1.3144438982199802E-4	6.572219491099901E-5	0.0	1.3144438982199802E-4	0.0
19	1.3144438982199802E-4	6.572219491099901E-5	0.0	2.6288877964399603E-4	0.0
20	1.3144438982199802E-4	6.572219491099901E-5	0.0	3.9433316946599405E-4	0.0
21	1.3144438982199802E-4	6.572219491099901E-5	0.0	5.91499754198991E-4	0.0
22	1.3144438982199802E-4	6.572219491099901E-5	0.0	0.0015773326778639762	0.0
23	1.3144438982199802E-4	1.3144438982199802E-4	0.0	0.002037388042240969	0.0
24	1.3144438982199802E-4	1.3144438982199802E-4	0.0	0.0031546653557279524	0.0
25	1.3144438982199802E-4	1.3144438982199802E-4	0.0	0.004337664864125935	0.0
26	1.3144438982199802E-4	1.9716658473299702E-4	0.0	0.006309330711455905	0.0
27	1.3144438982199802E-4	1.9716658473299702E-4	0.0	0.011961439473801818	0.0
28	1.3144438982199802E-4	1.9716658473299702E-4	0.0	0.028063377226996575	0.0
29	1.3144438982199802E-4	1.9716658473299702E-4	0.0	0.050934701056024226	0.0
30	1.3144438982199802E-4	1.9716658473299702E-4	0.0	0.0852416867995657	0.0
31	1.3144438982199802E-4	1.9716658473299702E-4	0.0	0.1909229762164521	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	790	0.0	23.183544	1
AAGACGG	395	0.0	20.607595	5
CTACCGA	70	1.2195265E-4	18.5	4
GTACTAG	65	0.0015802523	17.076923	1
TACCGAG	65	0.0015802523	17.076923	5
CGCAAGA	440	0.0	16.818182	2
GCGCAAG	465	0.0	16.311827	1
ACGGACC	465	0.0	16.311827	8
CGGACCA	465	0.0	16.311827	9
GACGGAC	460	0.0	16.086956	7
CGAACGA	265	0.0	16.056604	16
CAAGACG	520	0.0	16.009615	4
ACGAACG	270	0.0	15.759259	15
AGACGGA	475	0.0	15.578947	6
CTTAGGA	445	0.0	15.382023	2
GCGAAAG	490	0.0	15.102041	18
AGCGAAA	490	0.0	15.102041	17
GCTTAGG	435	0.0	14.885058	1
CGTCCTA	75	0.0041052187	14.8	33
CCAATAC	125	2.960087E-6	14.799999	3
>>END_MODULE