FastQCFastQC Report
Fri 10 Feb 2017
ERR1630425.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630425.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624115
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT16470.1014090750962832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16450.10128593110709525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC400.001931529123.1253
GGTATCA9300.021.0860211
TCGATTG450.00382647920.55555513
TCTAGAC3950.019.2025323
GGATACG609.239057E-418.51
GTTCGCG902.1533306E-618.530
GTCTAGA3650.018.2465741
CTAGACA4800.017.7291664
GCGGAAT1054.8002767E-717.61904727
TATACTG1054.8002767E-717.6190475
AGTCGGT1401.87174E-917.17857211
ATACCGA650.001580313817.0769236
GTTCTAG4600.016.8913041
CGCAATA1554.0199666E-1016.70967936
GACGGAC2150.016.3488377
CGAACGA1151.2428918E-616.08695616
GCAATTA3950.015.9240517
GTATCAA12350.015.8785422
AAGACGG2450.015.8571425
TTTCGGA2600.015.65384630
Produced by FastQC (version 0.11.5)