##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630425.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1624115
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.78176914812067	31.0	30.0	33.0	28.0	34.0
2	31.007426813987927	31.0	31.0	34.0	27.0	34.0
3	31.14684304990718	31.0	31.0	34.0	28.0	34.0
4	34.972122048007684	35.0	35.0	37.0	32.0	37.0
5	34.519306822484864	35.0	35.0	37.0	30.0	37.0
6	34.59833016750661	35.0	35.0	37.0	31.0	37.0
7	34.60372756855272	35.0	35.0	37.0	31.0	37.0
8	34.557512245130425	35.0	35.0	37.0	30.0	37.0
9	36.14275097514646	37.0	35.0	39.0	32.0	39.0
10	35.85622015682387	37.0	35.0	39.0	30.0	39.0
11	36.03353333969577	37.0	35.0	39.0	32.0	39.0
12	35.83684098724536	37.0	35.0	39.0	30.0	39.0
13	35.94045926550768	37.0	35.0	39.0	31.0	39.0
14	36.86619543566804	38.0	36.0	40.0	31.0	41.0
15	36.81564421238644	38.0	36.0	40.0	31.0	41.0
16	36.77934875301318	38.0	36.0	40.0	31.0	41.0
17	36.688274537209494	38.0	36.0	40.0	31.0	41.0
18	36.645278197664574	38.0	36.0	40.0	31.0	41.0
19	36.63083771777245	38.0	36.0	40.0	31.0	41.0
20	36.556876822146215	38.0	35.0	40.0	30.0	41.0
21	36.48338017935922	38.0	35.0	40.0	30.0	41.0
22	36.443807242713724	38.0	35.0	40.0	30.0	41.0
23	36.37266203440027	38.0	35.0	40.0	30.0	41.0
24	36.344239170255804	38.0	35.0	40.0	30.0	41.0
25	36.29780588197264	38.0	35.0	40.0	30.0	41.0
26	36.06299553910899	38.0	35.0	40.0	29.0	41.0
27	35.93516099537286	38.0	34.0	40.0	29.0	41.0
28	35.79947786948584	38.0	34.0	40.0	29.0	41.0
29	35.723112587470716	38.0	34.0	40.0	28.0	41.0
30	35.634274666510684	38.0	34.0	40.0	28.0	41.0
31	35.5059943415337	38.0	34.0	40.0	27.0	41.0
32	35.371494629382774	38.0	34.0	40.0	27.0	41.0
33	35.29040554394239	38.0	34.0	40.0	27.0	41.0
34	35.17515816306111	38.0	34.0	40.0	27.0	41.0
35	35.069964873177085	38.0	33.0	40.0	26.0	41.0
36	34.96442985872306	38.0	33.0	40.0	26.0	41.0
37	34.881856888212965	38.0	33.0	40.0	26.0	41.0
38	34.73782829417868	37.0	33.0	40.0	25.0	40.0
39	34.61423482943018	37.0	33.0	40.0	25.0	40.0
40	34.48897707366781	37.0	33.0	40.0	25.0	40.0
41	34.347003137093125	37.0	33.0	39.0	24.0	40.0
42	34.26326707160515	37.0	33.0	39.0	24.0	40.0
43	32.982585592768984	36.0	30.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	5.0
12	5.0
13	10.0
14	10.0
15	32.0
16	66.0
17	129.0
18	286.0
19	552.0
20	1043.0
21	1894.0
22	3041.0
23	5106.0
24	7938.0
25	11757.0
26	16545.0
27	23080.0
28	30660.0
29	40092.0
30	52112.0
31	65824.0
32	83254.0
33	105915.0
34	136573.0
35	180071.0
36	235854.0
37	287593.0
38	256480.0
39	78185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.983972809807184	19.644729591192743	12.12383359552741	26.247464003472658
2	20.526748413751488	21.097520803637675	32.482305748053555	25.893425034557282
3	21.226021556355306	22.033353549471556	28.728938529599198	28.011686364573933
4	16.28856330986414	15.481416032731673	32.805312431693565	35.42470822571062
5	15.90195275580855	35.565461805352456	33.069887292463896	15.462698146375104
6	36.921400270301056	33.975241900973764	14.515844013508897	14.587513815216289
7	31.286208181070922	29.55061679745585	19.857091400547375	19.306083620925858
8	28.052754884968124	33.01742795306983	18.945579592578113	19.984237569383943
9	27.32639006474295	13.542821782940248	18.420001046723907	40.71078710559289
10	17.512676134387036	26.156645311446542	32.257013819834185	24.073664734332237
11	38.06436120594909	20.637208572053087	20.135458388106752	21.162971833891074
12	22.662496190232833	24.686613940515294	28.027325651200808	24.62356421805106
13	31.49801584247421	18.8127688002389	23.69585897550358	25.99335638178331
14	23.808658869599753	19.52663450556149	23.781197760010837	32.883508864827924
15	26.699833447754624	26.663321254960394	20.698903710636255	25.937941586648726
16	26.41949615636824	25.496470385409904	22.46885226723477	25.61518119098709
17	25.127715709786564	25.489943753982942	23.999347336857305	25.3829931993732
18	25.310338245752302	24.20758382257414	24.814991549243743	25.667086382429815
19	25.773729077066587	24.352955301810525	24.597088260375653	25.27622736074724
20	25.925750331719122	24.14631968795313	24.617099158618693	25.31083082170905
21	26.231701572856604	24.208384258503862	24.422100651739562	25.13781351689997
22	26.235580608516024	24.249391206903454	24.216634905779454	25.29839327880107
23	25.391366990637977	24.38743561878315	24.67663927739107	25.544558113187797
24	25.660682894992043	24.70182222318001	24.278206900373434	25.359287981454514
25	26.339945139352817	24.019050375127378	24.432814178798914	25.20819030672089
26	26.404349445698116	24.294338762957057	24.08671799718616	25.214593794158663
27	25.87950976377904	24.17538166940149	24.175504813390678	25.76960375342879
28	25.50946207626923	23.99140454955468	24.82687494420038	25.67225842997571
29	25.30996881378474	24.46015214439864	24.56396252728409	25.665916514532526
30	25.095082552651753	24.84343781074616	24.61937732241867	25.44210231418342
31	26.141129168808856	24.441495830036665	24.21534189389298	25.202033107261496
32	25.354054361914024	24.475360427063357	24.318905988800054	25.85167922222256
33	25.166690782364547	24.266446649405985	25.060355947700746	25.506506620528718
34	26.26291857411575	23.91074523663657	24.348460546205164	25.47787564304252
35	25.64141086068413	24.223161537206416	25.065774283225018	25.069653318884438
36	26.155290727565472	23.987956517857416	24.397718141880347	25.45903461269676
37	25.82532640853634	23.893381934161066	24.887153926907885	25.394137730394707
38	25.228324348953123	23.929216835014763	25.348451310405974	25.494007505626143
39	25.430526779199745	23.82380558026987	25.329363992081845	25.41630364844854
40	25.117433186689365	24.06750753487284	25.535876461950046	25.27918281648775
41	25.312062261600932	23.629176505358306	25.60914713551688	25.44961409752388
42	25.27875181252559	23.806565421783557	25.642642300576007	25.27204046511485
43	25.016516687549835	23.288129227302253	25.675213885716218	26.02014019943169
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	44.0
2	51.0
3	122.5
4	194.0
5	194.0
6	291.0
7	388.0
8	366.5
9	345.0
10	499.5
11	654.0
12	654.0
13	1249.5
14	1845.0
15	2591.5
16	3338.0
17	3575.0
18	3812.0
19	3812.0
20	5246.0
21	6680.0
22	7156.0
23	7632.0
24	9344.5
25	11057.0
26	11057.0
27	13477.5
28	15898.0
29	19668.5
30	23439.0
31	27878.5
32	32318.0
33	32318.0
34	39657.5
35	46997.0
36	53820.5
37	60644.0
38	67493.5
39	74343.0
40	74343.0
41	80518.5
42	86694.0
43	93088.0
44	99482.0
45	106294.5
46	113107.0
47	113107.0
48	118303.0
49	123499.0
50	126273.0
51	129047.0
52	129535.0
53	130023.0
54	130023.0
55	124116.0
56	118209.0
57	113739.0
58	109269.0
59	106432.0
60	103595.0
61	103595.0
62	97832.5
63	92070.0
64	83456.0
65	74842.0
66	65876.5
67	56911.0
68	56911.0
69	47483.0
70	38055.0
71	32108.5
72	26162.0
73	20707.0
74	15252.0
75	15252.0
76	11706.5
77	8161.0
78	6272.5
79	4384.0
80	3563.0
81	2742.0
82	2742.0
83	2014.5
84	1287.0
85	1012.5
86	738.0
87	593.5
88	449.0
89	449.0
90	406.5
91	364.0
92	218.5
93	73.0
94	46.0
95	19.0
96	19.0
97	13.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1624115.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.12374108599386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.17587867235088	47.24265220293053
2	8.654193547250918	9.714114359079785
3	2.6389672994715596	4.4432615234983865
4	1.2374199336457938	2.7779454388233695
5	0.7323966568030668	2.055242016933141
6	0.44669636832728166	1.504216279203246
7	0.31985614507382737	1.2566066429621303
8	0.24168140798106272	1.085125181346209
9	0.18252866803108322	0.9219772534813055
>10	1.1419297303886826	12.630367916410624
>50	0.13204309685716356	5.210380239969738
>100	0.0928827751159473	9.505542165482176
>500	0.0030849863649866555	1.2769146980172015
>1k	4.407123378552365E-4	0.37565408186220334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	1647	0.1014090750962832	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1645	0.10128593110709525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.157199459397887E-5	0.0
10	0.0	0.0	0.0	6.157199459397887E-5	6.157199459397887E-5
11	0.0	0.0	0.0	6.157199459397887E-5	6.157199459397887E-5
12	0.0	0.0	0.0	6.157199459397887E-5	6.157199459397887E-5
13	0.0	0.0	0.0	6.157199459397887E-5	6.157199459397887E-5
14	0.0	0.0	0.0	6.157199459397887E-5	6.157199459397887E-5
15	0.0	0.0	0.0	1.2314398918795774E-4	6.157199459397887E-5
16	0.0	0.0	0.0	3.078599729698944E-4	1.2314398918795774E-4
17	0.0	0.0	0.0	6.157199459397888E-4	1.2314398918795774E-4
18	0.0	0.0	0.0	6.157199459397888E-4	1.2314398918795774E-4
19	0.0	0.0	0.0	6.157199459397888E-4	1.8471598378193662E-4
20	0.0	0.0	0.0	8.004359297217253E-4	1.8471598378193662E-4
21	0.0	0.0	0.0	0.0011082959026916198	1.8471598378193662E-4
22	0.0	0.0	0.0	0.0016008718594434507	1.8471598378193662E-4
23	0.0	0.0	0.0	0.0022781637999772183	1.8471598378193662E-4
24	0.0	0.0	0.0	0.0036327476810447536	1.8471598378193662E-4
25	0.0	0.0	0.0	0.004002179648608627	1.8471598378193662E-4
26	0.0	0.0	0.0	0.004802615578330352	1.8471598378193662E-4
27	0.0	0.0	0.0	0.008127503286405212	1.8471598378193662E-4
28	0.0	0.0	6.157199459397887E-5	0.020441902205200986	1.8471598378193662E-4
29	0.0	0.0	6.157199459397887E-5	0.03737420071854518	1.8471598378193662E-4
30	0.0	0.0	6.157199459397887E-5	0.06231085852910662	1.8471598378193662E-4
31	0.0	0.0	6.157199459397887E-5	0.14358589139315875	1.8471598378193662E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGAC	40	0.0019315291	23.125	3
GGTATCA	930	0.0	21.086021	1
TCGATTG	45	0.003826479	20.555555	13
TCTAGAC	395	0.0	19.202532	3
GGATACG	60	9.239057E-4	18.5	1
GTTCGCG	90	2.1533306E-6	18.5	30
GTCTAGA	365	0.0	18.246574	1
CTAGACA	480	0.0	17.729166	4
GCGGAAT	105	4.8002767E-7	17.619047	27
TATACTG	105	4.8002767E-7	17.619047	5
AGTCGGT	140	1.87174E-9	17.178572	11
ATACCGA	65	0.0015803138	17.076923	6
GTTCTAG	460	0.0	16.891304	1
CGCAATA	155	4.0199666E-10	16.709679	36
GACGGAC	215	0.0	16.348837	7
CGAACGA	115	1.2428918E-6	16.086956	16
GCAATTA	395	0.0	15.92405	17
GTATCAA	1235	0.0	15.878542	2
AAGACGG	245	0.0	15.857142	5
TTTCGGA	260	0.0	15.653846	30
>>END_MODULE