FastQCFastQC Report
Fri 10 Feb 2017
ERR1630424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347846
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47370.2017593998924972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36800.1567394113583259No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG35220.15000983880544125No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT30120.12828780081828195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29500.12564708247474493No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG26490.11282682083918621No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC24890.10601206382360683No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA24030.1023491319277329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGA450.003826978420.55555720
GGTATCA19500.019.6384621
ATACCCG500.00703701318.54
GTACGGT852.7250742E-517.4117646
ATATCGC700.002593573415.8571423
GTAGCGT855.3671096E-415.2352946
TCGCCAT7500.015.04666613
TAGTACC1601.0981239E-815.0312514
AACCGTT750.00410610114.87
TAGCGTG750.00410610114.87
CGAATTA2251.8189894E-1214.815
TACCGTC2800.014.5357147
GGTTAGA2550.014.5098041
GTACTAG1152.2116752E-514.4782611
GTCGCCA7700.014.41558412
ACAACCG800.00630120113.8750015
GCTTAGG12050.013.8174281
AAGACGG4300.013.7674425
GACCGTG1753.5706762E-813.7428577
AGGTCGC8100.013.70370410
Produced by FastQC (version 0.11.5)