FastQCFastQC Report
Fri 10 Feb 2017
ERR1630423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075555
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32520.1566809841223191No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31930.1538383709417481No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT24280.11698075936315828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22940.11052465485135302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG450.003826831520.5555554
TAGTTCG500.007036747418.4999987
GGTATCA16000.018.4999981
AAGACGG6200.016.7096795
TCAATAC1554.0199666E-1016.7096793
CTTATAC9500.016.55263137
GCGCAAG6950.015.7050361
GTTCTAA1455.3518306E-815.3103451
GACGGAC6450.015.201557
ATTAGAC1101.4529105E-515.1363643
AGACGGA6850.014.8540146
GCGCGTT1252.961342E-614.80000125
CAAGACG7550.014.7019874
CGCAAGA7300.014.698632
TCTTATA15400.014.41558437
CGGACCA6900.014.2101449
TATAGTA2500.014.0600012
GTATCAA21350.013.8641682
TGACCCG5750.013.83478313
ATTAGAG4550.013.8241773
Produced by FastQC (version 0.11.5)