FastQCFastQC Report
Fri 10 Feb 2017
ERR1630422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757507
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45640.2596860211652073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38260.21769472326426012No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG31720.18048292268537197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30620.17422405714458036No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA24670.14036928444666222No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC24640.14019858811373156No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT24330.1384347260067812No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA23870.13581738223517745No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG21810.1240962340406041No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC20800.11834945749860455No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC18320.10423856064300172No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA17650.10042634254088319No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC17630.10031254498559608No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.10008494987502183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.021.7713431
AAGACGG2650.016.7547155
TATACTG1554.0199666E-1016.7096775
CGGCCTT6150.015.34146324
TTAGTAC855.3661555E-415.2352943
GTATCAA23600.015.2076272
TCGCCAT5900.015.05084713
CTTATAC4800.015.03124937
GTAAGTC750.00410555414.83
AGACTAT1252.9607163E-614.7999996
GACGGAC2900.014.6724147
TTCCTCG6750.014.52592619
ATCTCGC6500.014.51538511
TCTAGCG2850.014.28070228
ATTATAC1051.6564173E-414.0952393
GCGTTAT2405.456968E-1213.8749991
CAAGACG3350.013.805974
CGCAAGA2950.013.796612
TAGAACA10100.013.7376234
CGGTCCT2355.638867E-1113.382977512
Produced by FastQC (version 0.11.5)