##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630422.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1757507
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8578133685954	33.0	31.0	34.0	30.0	34.0
2	32.07643383497192	33.0	31.0	34.0	30.0	34.0
3	32.188411767350004	34.0	31.0	34.0	30.0	34.0
4	35.828583328544354	37.0	35.0	37.0	35.0	37.0
5	35.7568248661314	37.0	35.0	37.0	35.0	37.0
6	35.822436553595516	37.0	35.0	37.0	35.0	37.0
7	35.79859937968953	37.0	35.0	37.0	35.0	37.0
8	35.80268584989989	37.0	35.0	37.0	35.0	37.0
9	37.54883195344314	39.0	37.0	39.0	35.0	39.0
10	37.45689149460002	39.0	37.0	39.0	35.0	39.0
11	37.51199454682115	39.0	37.0	39.0	35.0	39.0
12	37.43953793640651	39.0	37.0	39.0	35.0	39.0
13	37.441507772088535	39.0	37.0	39.0	34.0	39.0
14	38.681966558312425	40.0	38.0	41.0	34.0	41.0
15	38.702219109226874	40.0	38.0	41.0	35.0	41.0
16	38.64807650837237	40.0	38.0	41.0	34.0	41.0
17	38.62752182494864	40.0	38.0	41.0	34.0	41.0
18	38.57889726755	40.0	38.0	41.0	34.0	41.0
19	38.62126864928561	40.0	38.0	41.0	34.0	41.0
20	38.60336146598563	40.0	38.0	41.0	34.0	41.0
21	38.540765982724395	40.0	38.0	41.0	34.0	41.0
22	38.51412483705612	40.0	38.0	41.0	34.0	41.0
23	38.452861923167305	40.0	38.0	41.0	34.0	41.0
24	38.41776505015343	40.0	38.0	41.0	34.0	41.0
25	38.4015033795029	40.0	38.0	41.0	34.0	41.0
26	38.2480115299683	40.0	38.0	41.0	34.0	41.0
27	38.128687396408665	40.0	38.0	41.0	33.0	41.0
28	38.062520376874744	40.0	38.0	41.0	33.0	41.0
29	38.01977915308446	40.0	38.0	41.0	33.0	41.0
30	37.96377766916434	40.0	37.0	41.0	33.0	41.0
31	37.90499155906634	40.0	37.0	41.0	33.0	41.0
32	37.81791708368729	40.0	37.0	41.0	33.0	41.0
33	37.728067085934796	40.0	37.0	41.0	33.0	41.0
34	37.683699126091675	40.0	37.0	41.0	33.0	41.0
35	37.56487399481197	40.0	37.0	41.0	32.0	41.0
36	37.478289986896215	40.0	37.0	41.0	32.0	41.0
37	37.43252971396416	40.0	37.0	41.0	32.0	41.0
38	37.31724141070277	40.0	36.0	41.0	31.0	41.0
39	37.2488638736574	40.0	36.0	41.0	31.0	41.0
40	37.15360963000432	39.0	36.0	41.0	31.0	41.0
41	37.04810450257097	39.0	36.0	41.0	31.0	41.0
42	37.01393678659601	39.0	36.0	41.0	31.0	41.0
43	35.80786932854321	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	6.0
12	1.0
13	2.0
14	0.0
15	1.0
16	17.0
17	34.0
18	83.0
19	166.0
20	299.0
21	557.0
22	983.0
23	1689.0
24	2720.0
25	4248.0
26	6554.0
27	9710.0
28	14078.0
29	19405.0
30	26008.0
31	34329.0
32	44775.0
33	57278.0
34	75995.0
35	102781.0
36	143480.0
37	217811.0
38	404034.0
39	590460.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.049619148031844	19.807545574498423	12.758640506126007	25.384194771343726
2	18.597877561796338	21.76065301589126	34.77163960086646	24.869829821445947
3	19.942395677513662	23.3196795233248	30.684998694173053	26.05292610498849
4	15.184064700738034	15.94127363361853	34.301257406087146	34.57340425955629
5	14.481933784616505	36.8095262209482	34.219095571169845	14.489444423265455
6	34.118384734740744	34.90700179288048	15.994473990715258	14.980139481663516
7	28.10845134613973	31.1400182189886	21.53573214786627	19.215798287005402
8	26.228231238908293	33.93625174750371	20.592179718203113	19.243337295384883
9	25.93036613794426	14.789244082669374	20.36356042963129	38.916829349755076
10	16.93301932794578	27.250417779274848	33.32117596117683	22.49538693160255
11	35.00321762587574	22.187792139661465	21.7788606247372	21.030129609725595
12	20.828992430755612	26.145045225993407	29.283638699589815	23.742323643661166
13	29.258034249650215	20.220630700190668	25.653439787153054	24.867895263006066
14	22.559625651562126	21.896242803015863	24.90266041614628	30.641471129275732
15	25.204508431545364	28.468222317179958	22.693622272912712	23.633646978361963
16	24.91677131300188	27.056506745065594	23.929463723330834	24.097258218601688
17	23.647587178884635	26.959096037739823	25.342886258774506	24.05043052460104
18	22.641389195035924	25.51659822692029	27.337302212736564	24.504710365307222
19	23.938681325309087	26.001318913665777	27.07943695245595	22.980562808569182
20	24.29902128412575	25.089459103150087	27.241882962628317	23.369636650095845
21	24.134697614291152	26.25548575339956	25.84945607613512	23.76036055617417
22	24.20707285945376	25.496057768190965	26.214461734718554	24.08240763763672
23	23.304089258250464	26.01013822420053	26.47591161799071	24.209860899558294
24	24.638365593991946	26.040408373906903	26.30248414373314	23.018741888368012
25	24.423914101053366	25.319273266052427	26.378216416776716	23.87859621611749
26	24.244057064922075	25.988061498474828	26.059867755861	23.708013680742095
27	24.358708101873848	25.316712821058463	25.991873716576947	24.332705360490742
28	23.28747481517855	25.843026514261396	26.88268097936452	23.98681769119554
29	23.424202577856022	26.23551428244667	26.565356496446384	23.774926643250925
30	23.068357622473197	27.18845501042101	26.3730955267888	23.370091840316995
31	24.14476869793406	26.032442545036805	26.34470303674466	23.478085720284472
32	23.127475452444855	26.091560375008466	26.189767665221247	24.59119650732543
33	23.370774625648714	25.63500458319654	26.764331521865913	24.22988926928883
34	23.795922292201396	25.562629338033933	26.56467371111466	24.076774658650006
35	24.07011750166571	25.608262157704065	26.68495772705315	23.636662613577073
36	23.386080396834835	26.372981729233512	26.534744954074153	23.706192919857504
37	24.42840910448721	25.200696213443248	26.19585583442911	24.175038847640437
38	23.40440180323606	25.85981165366624	27.195112167405306	23.540674375692387
39	23.664941306065923	25.268121264950867	27.18782912386693	23.879108305116283
40	23.36730380021246	24.735036617208355	28.0159339336913	23.881725648887887
41	22.606396446785133	25.689058421957924	28.10765476325272	23.596890368004225
42	23.596548975338365	24.693272914417978	27.75858076240948	23.951597347834177
43	22.67837340050424	24.76598955224645	27.855024190515316	24.700612856734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	214.0
1	221.5
2	229.0
3	482.5
4	736.0
5	736.0
6	959.5
7	1183.0
8	1155.0
9	1127.0
10	1667.0
11	2207.0
12	2207.0
13	3717.5
14	5228.0
15	8291.0
16	11354.0
17	11440.5
18	11527.0
19	11527.0
20	13420.0
21	15313.0
22	16848.5
23	18384.0
24	20761.0
25	23138.0
26	23138.0
27	26671.0
28	30204.0
29	41026.0
30	51848.0
31	53486.0
32	55124.0
33	55124.0
34	60669.5
35	66215.0
36	70967.0
37	75719.0
38	87341.5
39	98964.0
40	98964.0
41	107383.0
42	115802.0
43	109335.0
44	102868.0
45	109231.5
46	115595.0
47	115595.0
48	121860.5
49	128126.0
50	131032.5
51	133939.0
52	142332.0
53	150725.0
54	150725.0
55	133590.5
56	116456.0
57	109999.5
58	103543.0
59	94254.5
60	84966.0
61	84966.0
62	78058.0
63	71150.0
64	62536.0
65	53922.0
66	46008.5
67	38095.0
68	38095.0
69	32174.5
70	26254.0
71	22858.0
72	19462.0
73	15335.0
74	11208.0
75	11208.0
76	8716.5
77	6225.0
78	5241.0
79	4257.0
80	3395.0
81	2533.0
82	2533.0
83	1920.5
84	1308.0
85	1130.0
86	952.0
87	855.5
88	759.0
89	759.0
90	641.0
91	523.0
92	305.0
93	87.0
94	58.0
95	29.0
96	29.0
97	17.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1757507.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.75421684799493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34678677778638	36.630426583522464
2	13.026579660340671	12.180950604544957
3	3.9488600039696333	5.538775707839112
4	1.5879931973423311	2.9698151320673665
5	0.7977131027215388	1.864822569356484
6	0.4497726620787272	1.2617261145077254
7	0.29678209084399176	0.9713069962344926
8	0.2187086488592513	0.8180441276237932
9	0.15012586425210825	0.6317115490562148
>10	0.8915171844874029	8.078636124317082
>50	0.11572862902983318	3.8474300958122063
>100	0.14625588277415355	15.115284168568888
>500	0.017321231384213626	5.4902630754461565
>1k	0.005855064129875029	4.600807151103091
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4564	0.2596860211652073	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3826	0.21769472326426012	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3172	0.18048292268537197	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3062	0.17422405714458036	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2467	0.14036928444666222	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2464	0.14019858811373156	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2433	0.1384347260067812	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2387	0.13581738223517745	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2181	0.1240962340406041	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2080	0.11834945749860455	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	1832	0.10423856064300172	No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA	1765	0.10042634254088319	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1763	0.10031254498559608	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1759	0.10008494987502183	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.689877764355988E-5	0.0
6	0.0	0.0	0.0	5.689877764355988E-5	0.0
7	0.0	0.0	0.0	5.689877764355988E-5	0.0
8	0.0	0.0	0.0	5.689877764355988E-5	0.0
9	0.0	0.0	0.0	5.689877764355988E-5	0.0
10	0.0	0.0	0.0	5.689877764355988E-5	0.0
11	0.0	0.0	0.0	5.689877764355988E-5	0.0
12	0.0	0.0	0.0	5.689877764355988E-5	0.0
13	0.0	0.0	0.0	5.689877764355988E-5	0.0
14	0.0	0.0	0.0	5.689877764355988E-5	0.0
15	0.0	0.0	0.0	5.689877764355988E-5	0.0
16	0.0	0.0	0.0	2.2759511057423952E-4	0.0
17	0.0	0.0	0.0	4.5519022114847904E-4	0.0
18	0.0	0.0	0.0	4.5519022114847904E-4	0.0
19	0.0	0.0	0.0	4.5519022114847904E-4	0.0
20	0.0	0.0	0.0	6.258865540791587E-4	0.0
21	0.0	0.0	0.0	7.965828870098384E-4	0.0
22	0.0	0.0	0.0	9.67279219940518E-4	0.0
23	0.0	0.0	0.0	0.0016500645516632367	0.0
24	0.0	0.0	0.0	0.002446647438673075	0.0
25	0.0	0.0	0.0	0.003413926658613593	0.0
26	0.0	0.0	0.0	0.00483639609970259	0.0
27	0.0	0.0	0.0	0.012119439638078254	0.0
28	0.0	0.0	0.0	0.031237428926314377	0.0
29	0.0	0.0	0.0	0.057012575198847004	0.0
30	0.0	0.0	0.0	0.09200532344963633	0.0
31	0.0	0.0	0.0	0.19772325231137058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1640	0.0	21.771343	1
AAGACGG	265	0.0	16.754715	5
TATACTG	155	4.0199666E-10	16.709677	5
CGGCCTT	615	0.0	15.341463	24
TTAGTAC	85	5.3661555E-4	15.235294	3
GTATCAA	2360	0.0	15.207627	2
TCGCCAT	590	0.0	15.050847	13
CTTATAC	480	0.0	15.031249	37
GTAAGTC	75	0.004105554	14.8	3
AGACTAT	125	2.9607163E-6	14.799999	6
GACGGAC	290	0.0	14.672414	7
TTCCTCG	675	0.0	14.525926	19
ATCTCGC	650	0.0	14.515385	11
TCTAGCG	285	0.0	14.280702	28
ATTATAC	105	1.6564173E-4	14.095239	3
GCGTTAT	240	5.456968E-12	13.874999	1
CAAGACG	335	0.0	13.80597	4
CGCAAGA	295	0.0	13.79661	2
TAGAACA	1010	0.0	13.737623	4
CGGTCCT	235	5.638867E-11	13.3829775	12
>>END_MODULE