FastQCFastQC Report
Fri 10 Feb 2017
ERR1630421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920270
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30180.15716539861581966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25370.13211683773636No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21530.1121196498409078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG451.3236637E-424.6666665
GGTATCA11500.016.8913041
TCGCACG2200.016.81818222
CGATCGC2150.016.34883719
CCGATCG2050.016.24390418
TATACTG2550.015.2352945
ATCGGAT750.00410573814.836
TAGATAA2950.014.4237294
CTAATAC4400.014.2954543
TACACCG1051.6565439E-414.0952385
ATACCGC3950.014.05063227
CTAGATA3050.013.950823
TATCGCG800.006300651513.8757
TACCGCA4050.013.70370428
GTACTAT2850.013.6315781
ATCGCAC2750.013.45454621
GTACTGG3850.013.4545451
TCGTTTA1805.163747E-813.36111130
GTCTATA1254.8476875E-513.3200011
CGGGTAA2102.042725E-913.21428524
Produced by FastQC (version 0.11.5)