##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630421.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1920270
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.750592885375493	31.0	31.0	34.0	30.0	34.0
2	31.966983809568447	33.0	31.0	34.0	30.0	34.0
3	32.10380154874054	34.0	31.0	34.0	30.0	34.0
4	35.7578788399548	37.0	35.0	37.0	33.0	37.0
5	35.68027725267801	37.0	35.0	37.0	33.0	37.0
6	35.73210590177423	37.0	35.0	37.0	33.0	37.0
7	35.7032110067855	37.0	35.0	37.0	33.0	37.0
8	35.70390570076083	37.0	35.0	37.0	33.0	37.0
9	37.44274919672754	39.0	37.0	39.0	35.0	39.0
10	37.3089070807751	39.0	37.0	39.0	34.0	39.0
11	37.390769527201904	39.0	37.0	39.0	34.0	39.0
12	37.29302650148156	39.0	37.0	39.0	34.0	39.0
13	37.3085461940248	39.0	37.0	39.0	34.0	39.0
14	38.530040567211906	40.0	38.0	41.0	34.0	41.0
15	38.5324183578351	40.0	38.0	41.0	34.0	41.0
16	38.49108823238399	40.0	38.0	41.0	34.0	41.0
17	38.45412051430267	40.0	38.0	41.0	34.0	41.0
18	38.399595369401176	40.0	38.0	41.0	34.0	41.0
19	38.41955818712994	40.0	38.0	41.0	34.0	41.0
20	38.38774599405292	40.0	38.0	41.0	34.0	41.0
21	38.335459596827526	40.0	38.0	41.0	34.0	41.0
22	38.295822983226316	40.0	38.0	41.0	34.0	41.0
23	38.235568435688734	40.0	38.0	41.0	34.0	41.0
24	38.20043066860389	40.0	38.0	41.0	33.0	41.0
25	38.17692720294542	40.0	38.0	41.0	33.0	41.0
26	38.00315997229556	40.0	37.0	41.0	33.0	41.0
27	37.88318621860468	40.0	37.0	41.0	33.0	41.0
28	37.80587209090388	40.0	37.0	41.0	33.0	41.0
29	37.74348242695038	40.0	37.0	41.0	32.0	41.0
30	37.6805261760065	40.0	37.0	41.0	32.0	41.0
31	37.620961114843226	40.0	37.0	41.0	32.0	41.0
32	37.53535232024663	40.0	37.0	41.0	32.0	41.0
33	37.43575174324444	40.0	37.0	41.0	32.0	41.0
34	37.36738948168747	40.0	37.0	41.0	31.0	41.0
35	37.255107354694914	39.0	36.0	41.0	31.0	41.0
36	37.1615225983846	39.0	36.0	41.0	31.0	41.0
37	37.08930567055675	39.0	36.0	41.0	31.0	41.0
38	36.9849974222375	39.0	36.0	41.0	31.0	41.0
39	36.906853202935004	39.0	36.0	41.0	30.0	41.0
40	36.80084363136434	39.0	35.0	41.0	30.0	41.0
41	36.686936212095176	39.0	35.0	41.0	30.0	41.0
42	36.63805037833222	39.0	35.0	40.0	30.0	41.0
43	35.3567977419842	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	5.0
11	5.0
12	4.0
13	3.0
14	3.0
15	4.0
16	17.0
17	33.0
18	86.0
19	151.0
20	331.0
21	697.0
22	1266.0
23	2172.0
24	3770.0
25	5834.0
26	8421.0
27	12061.0
28	17608.0
29	23935.0
30	31896.0
31	41601.0
32	53731.0
33	69003.0
34	90936.0
35	120471.0
36	167368.0
37	252785.0
38	466724.0
39	549343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.0538413868883	18.298155988480787	11.778395746431492	26.86960687819942
2	18.75397730527478	20.341149942456006	33.683700729584906	27.22117202268431
3	19.812370135449704	22.454498586136324	29.11559311971754	28.617538158696433
4	14.697151963005203	15.256344159935843	34.04531654402766	36.00118733303129
5	15.384242840850506	35.65399657339853	33.79779926781129	15.163961317939664
6	35.739192925994786	34.62153759627552	15.368255505736172	14.27101397199352
7	30.667041613939706	29.694626276513198	20.732292854650648	18.90603925489645
8	28.453290422700974	33.07378649877361	19.15730600384321	19.315617074682205
9	27.32287647049634	13.765564217531908	18.44662469340249	40.464934618569266
10	17.076088258421994	26.03566165174689	32.65280403276623	24.23544605706489
11	37.76708483702812	20.89836325100116	20.831862186046752	20.502689725923958
12	22.250621006420975	24.28070010988038	29.160274336421445	24.308404547277206
13	31.059955110479255	18.90723700312977	24.000843631364337	26.031964255026637
14	23.19241564988257	19.271769074140614	24.405057622105225	33.13075765387159
15	25.75929426591052	26.873668806990686	21.28195514172486	26.08508178537393
16	26.023996625474545	25.33628083550751	22.952918079228443	25.68680445978951
17	24.754123118103184	25.572966301613835	24.498169528243423	25.174741052039558
18	25.123966942148762	24.640337035937655	25.157087284600603	25.07860873731298
19	25.676805865841786	24.536341243679274	24.97091554833435	24.815937342144593
20	25.5137038020695	24.312258172027892	24.945346227353447	25.228691798549164
21	25.920365365287175	24.41979513297609	24.558942232081947	25.100897269654787
22	25.842355502090854	24.181287006514708	24.76162206356398	25.21473542783046
23	25.333312502929278	24.291427767970127	24.86697183208611	25.50828789701448
24	25.189270258869843	24.73292818197441	24.592687486655525	25.48511407250022
25	25.66201627896077	24.03995271498279	24.86718013612669	25.43085086992975
26	25.45793039520484	24.643149140485452	24.795992230259287	25.10292823405042
27	25.617282986246725	24.350013279382587	24.54279866893718	25.489905065433504
28	25.225098553849197	24.60601894525249	24.974508793034314	25.194373707864
29	25.110531331531504	24.667260333182313	24.984715691022615	25.237492644263565
30	24.95774031776781	24.957167481656224	24.81234409744463	25.27274810313133
31	25.50245538387831	24.592114650543934	24.606800085404657	25.298629880173102
32	25.050487691835006	24.584667781093284	24.703244856192097	25.661599670879614
33	24.91373608919579	24.4714024590292	25.10928150728804	25.50557994448697
34	25.458451155306282	24.152072364823695	24.87108583688752	25.518390642982496
35	25.32123086857577	24.470569242866887	25.218016216469557	24.990183672087777
36	25.172814239664216	24.6557515349404	24.80583459617658	25.36559962921881
37	25.176876168455475	24.26039046592406	25.17146026340046	25.39127310222
38	24.854161133590587	24.4066719784197	25.604732667801922	25.134434220187785
39	24.823644591645966	24.204356679008683	25.42210210022549	25.54989662911986
40	24.808594624714235	24.1930041087972	25.976190848161977	25.02221041832659
41	24.402557973618293	24.11869164232113	26.16835132559484	25.310399058465737
42	24.547381357829888	24.240809886109766	26.034776359574437	25.17703239648591
43	24.54274659292704	23.463106750613193	26.366708848234882	25.627437808224883
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	95.0
2	109.0
3	239.5
4	370.0
5	370.0
6	563.5
7	757.0
8	688.0
9	619.0
10	850.0
11	1081.0
12	1081.0
13	2002.5
14	2924.0
15	4771.0
16	6618.0
17	6748.5
18	6879.0
19	6879.0
20	8295.0
21	9711.0
22	10101.0
23	10491.0
24	13149.0
25	15807.0
26	15807.0
27	20173.0
28	24539.0
29	29241.0
30	33943.0
31	39224.5
32	44506.0
33	44506.0
34	52671.5
35	60837.0
36	68852.0
37	76867.0
38	84539.5
39	92212.0
40	92212.0
41	100543.0
42	108874.0
43	112531.0
44	116188.0
45	121674.0
46	127160.0
47	127160.0
48	132874.5
49	138589.0
50	140327.5
51	142066.0
52	141735.0
53	141404.0
54	141404.0
55	140024.0
56	138644.0
57	133503.0
58	128362.0
59	123972.5
60	119583.0
61	119583.0
62	110933.5
63	102284.0
64	92271.0
65	82258.0
66	72543.0
67	62828.0
68	62828.0
69	54151.5
70	45475.0
71	38757.0
72	32039.0
73	25769.0
74	19499.0
75	19499.0
76	15502.0
77	11505.0
78	9203.5
79	6902.0
80	5372.5
81	3843.0
82	3843.0
83	2878.5
84	1914.0
85	1530.0
86	1146.0
87	949.5
88	753.0
89	753.0
90	606.5
91	460.0
92	276.0
93	92.0
94	64.5
95	37.0
96	37.0
97	23.0
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1920270.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.79198009150286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.132843382233	47.111685328037595
2	11.200588123299749	13.170095079163243
3	3.5112160072143075	6.192940247793292
4	1.5837636086140554	3.724503941891371
5	0.899623558496236	2.6445325170478835
6	0.5529005989741331	1.9503672604480347
7	0.36581954173984066	1.5055078650535987
8	0.27537924808295056	1.2952073016724686
9	0.20133716037454896	1.0653309291978184
>10	1.1432460319540034	12.420211529702467
>50	0.08910176585841027	3.55852745396349
>100	0.04214114225068486	4.286191299435281
>500	0.0016850777067108223	0.6207458418964765
>1k	3.5475320141280416E-4	0.4541534046970435
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3018	0.15716539861581966	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2537	0.13211683773636	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2153	0.1121196498409078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.207601014440678E-5	0.0
3	0.0	0.0	0.0	5.207601014440678E-5	0.0
4	0.0	0.0	0.0	5.207601014440678E-5	0.0
5	0.0	0.0	0.0	5.207601014440678E-5	0.0
6	0.0	0.0	0.0	5.207601014440678E-5	0.0
7	0.0	0.0	0.0	5.207601014440678E-5	0.0
8	0.0	0.0	0.0	5.207601014440678E-5	0.0
9	0.0	0.0	0.0	1.0415202028881356E-4	0.0
10	0.0	0.0	0.0	1.5622803043322032E-4	0.0
11	0.0	0.0	0.0	1.5622803043322032E-4	0.0
12	0.0	0.0	0.0	1.5622803043322032E-4	0.0
13	0.0	0.0	0.0	1.5622803043322032E-4	0.0
14	0.0	0.0	0.0	2.0830404057762712E-4	0.0
15	0.0	0.0	0.0	2.0830404057762712E-4	0.0
16	0.0	0.0	0.0	2.0830404057762712E-4	0.0
17	0.0	0.0	0.0	2.603800507220339E-4	0.0
18	0.0	0.0	0.0	3.1245606086644065E-4	0.0
19	0.0	0.0	0.0	4.68684091299661E-4	0.0
20	0.0	0.0	0.0	5.207601014440678E-4	0.0
21	0.0	0.0	0.0	5.728361115884745E-4	0.0
22	0.0	0.0	0.0	6.769881318772881E-4	0.0
23	0.0	0.0	0.0	9.894441927437287E-4	0.0
24	0.0	0.0	0.0	0.001666432324621017	0.0
25	0.0	0.0	0.0	0.002135116415920678	0.0
26	0.0	0.0	0.0	0.0033849406593864403	0.0
27	0.0	0.0	0.0	0.00838423763324949	0.0
28	0.0	5.207601014440678E-5	0.0	0.019840959865018982	0.0
29	0.0	5.207601014440678E-5	0.0	0.03780718336483932	0.0
30	0.0	5.207601014440678E-5	0.0	0.0660323808631078	0.0
31	0.0	5.207601014440678E-5	0.0	0.153624229926	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	45	1.3236637E-4	24.666666	5
GGTATCA	1150	0.0	16.891304	1
TCGCACG	220	0.0	16.818182	22
CGATCGC	215	0.0	16.348837	19
CCGATCG	205	0.0	16.243904	18
TATACTG	255	0.0	15.235294	5
ATCGGAT	75	0.004105738	14.8	36
TAGATAA	295	0.0	14.423729	4
CTAATAC	440	0.0	14.295454	3
TACACCG	105	1.6565439E-4	14.095238	5
ATACCGC	395	0.0	14.050632	27
CTAGATA	305	0.0	13.95082	3
TATCGCG	80	0.0063006515	13.875	7
TACCGCA	405	0.0	13.703704	28
GTACTAT	285	0.0	13.631578	1
ATCGCAC	275	0.0	13.454546	21
GTACTGG	385	0.0	13.454545	1
TCGTTTA	180	5.163747E-8	13.361111	30
GTCTATA	125	4.8476875E-5	13.320001	1
CGGGTAA	210	2.042725E-9	13.214285	24
>>END_MODULE