FastQCFastQC Report
Fri 10 Feb 2017
ERR1630420.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630420.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015943
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG48320.2396893166126225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44720.22183166885174826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41970.20819041014552495No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT34790.17257432377800364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32040.15893306507178032No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG30600.15179000596743064No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC28320.14048016238554362No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA27370.13576772755975738No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC24780.12292014208735069No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT22760.11290001751041573No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC22700.1126023900477345No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA22160.10992374288360336No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC22020.1092292788040138No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC21720.10774114149060762No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA21300.10565774925183898No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT20890.10362396159018385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.019.7191961
GTCGTTA650.001580492817.07692334
TTGCGCG650.001580492817.07692318
TCTATAC1655.4569682E-1116.8181823
AACGCCG2200.015.9772735
TAGCGAG700.002593403715.85714228
CTAGTAC957.063897E-515.5789483
TCGTTTA1557.21775E-915.516129530
TAGGACA13950.015.38351254
CTTATAC4750.015.18947237
TAACGCC2201.8189894E-1215.1363644
GCTTAGG12650.015.0632411
TAGTACG1601.0977601E-815.031254
ATTAGAC1752.240995E-914.83
ACTCGTC1508.110146E-814.88
AATACCG750.00410583314.85
TTAGGAC13700.014.5839423
CGAATTA2550.014.50980515
CTTAGGA13250.014.3811322
ATGGTCG1551.211447E-714.32258136
Produced by FastQC (version 0.11.5)