##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630420.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2015943
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72087306039903	31.0	31.0	34.0	30.0	34.0
2	31.94612347670544	33.0	31.0	34.0	30.0	34.0
3	32.069799592548	33.0	31.0	34.0	30.0	34.0
4	35.7371240159072	37.0	35.0	37.0	33.0	37.0
5	35.655809712873825	37.0	35.0	37.0	33.0	37.0
6	35.71383119463199	37.0	35.0	37.0	33.0	37.0
7	35.695806379446246	37.0	35.0	37.0	33.0	37.0
8	35.699313423048174	37.0	35.0	37.0	33.0	37.0
9	37.42549962970183	39.0	37.0	39.0	34.0	39.0
10	37.303935676752765	39.0	37.0	39.0	34.0	39.0
11	37.37418766304405	39.0	37.0	39.0	34.0	39.0
12	37.301476281819475	39.0	37.0	39.0	34.0	39.0
13	37.30508451875871	39.0	37.0	39.0	34.0	39.0
14	38.525955346951775	40.0	38.0	41.0	34.0	41.0
15	38.53793584441624	40.0	38.0	41.0	34.0	41.0
16	38.49098709636136	40.0	38.0	41.0	34.0	41.0
17	38.46322490268822	40.0	38.0	41.0	34.0	41.0
18	38.405464341005676	40.0	38.0	41.0	34.0	41.0
19	38.453347639293376	40.0	38.0	41.0	34.0	41.0
20	38.429161439584355	40.0	38.0	41.0	34.0	41.0
21	38.36324340519548	40.0	38.0	41.0	34.0	41.0
22	38.3523338705509	40.0	38.0	41.0	34.0	41.0
23	38.29880457929614	40.0	38.0	41.0	34.0	41.0
24	38.26520491898828	40.0	38.0	41.0	34.0	41.0
25	38.2434721616633	40.0	38.0	41.0	34.0	41.0
26	38.08133563300153	40.0	38.0	41.0	33.0	41.0
27	37.96544445949117	40.0	37.0	41.0	33.0	41.0
28	37.91346481522543	40.0	37.0	41.0	33.0	41.0
29	37.86029614924628	40.0	37.0	41.0	33.0	41.0
30	37.80312885830601	40.0	37.0	41.0	33.0	41.0
31	37.747067749435374	40.0	37.0	41.0	33.0	41.0
32	37.655563178125576	40.0	37.0	41.0	32.0	41.0
33	37.57465364844145	40.0	37.0	41.0	32.0	41.0
34	37.51662373390518	40.0	37.0	41.0	32.0	41.0
35	37.41053591296976	40.0	37.0	41.0	32.0	41.0
36	37.33109963922591	40.0	36.0	41.0	31.0	41.0
37	37.2736545626538	39.0	36.0	41.0	31.0	41.0
38	37.16717536160497	39.0	36.0	41.0	31.0	41.0
39	37.086327341596466	39.0	36.0	41.0	31.0	41.0
40	37.004710450642705	39.0	36.0	41.0	31.0	41.0
41	36.896104205327234	39.0	36.0	41.0	30.0	41.0
42	36.87452968660324	39.0	36.0	41.0	30.0	41.0
43	35.64664278702325	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	6.0
12	0.0
13	1.0
14	7.0
15	8.0
16	18.0
17	41.0
18	77.0
19	167.0
20	271.0
21	605.0
22	1169.0
23	1958.0
24	3269.0
25	5414.0
26	8122.0
27	12097.0
28	17383.0
29	23615.0
30	32231.0
31	42571.0
32	54947.0
33	70201.0
34	92461.0
35	123370.0
36	173537.0
37	264988.0
38	500239.0
39	587164.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.30218810750105	19.20709067667092	12.453774734702321	26.036946481125707
2	17.829918802267724	21.588606423891946	35.297724191606605	25.283750582233722
3	19.38090511487676	23.526310019678135	31.00935889556401	26.08342596988109
4	14.841788681525223	15.74082203713101	34.96358776016981	34.45380152117396
5	14.050149235370245	37.39689068589737	34.599688582464886	13.953271496267503
6	34.07239192774796	35.46811591399162	16.097131714537564	14.362360443722864
7	28.497879156305512	31.322611800036015	21.600263499513627	18.579245544144847
8	26.660029574248874	34.823306016092715	20.231524403219733	18.285140006438674
9	26.436213722312583	14.756468808889933	19.377780026518607	39.42953744227887
10	16.176697456227682	27.698104559503918	33.776897461882605	22.348300522385802
11	34.86145193589303	22.24958741392986	22.080981456320938	20.807979193856173
12	20.638331540127872	25.90276610003358	29.900895015384858	23.55800734445369
13	29.429701137383347	20.038612202825178	25.854748869387677	24.676937790403798
14	22.374789366564432	21.05119043544386	25.301161788800574	31.272858409191134
15	24.67822750940875	28.337904395114343	22.771973215512542	24.211894879964362
16	24.61269986304176	27.251762574636285	23.91679725071592	24.218740311606034
17	23.477598324952638	27.234996227571912	25.596904277551495	23.69050116992395
18	22.67623638168341	26.107633003512497	27.03191508886908	24.18421552593501
19	24.136942363945806	26.196028359928825	27.109397438320425	22.557631837804937
20	24.341610849116268	25.435639797355382	27.09223425463914	23.130515098889205
21	23.519166960573788	26.487703273356438	25.902071635953995	24.091058130115783
22	23.806575880369635	25.87429307277041	26.198508588784502	24.120622458075452
23	23.263504970130604	26.456055553158002	26.291864402912186	23.988575073799208
24	24.510514434187872	26.176930597740117	26.136552471969694	23.17600249610232
25	24.2988517036444	25.604295359541418	26.37520009246293	23.721652844351254
26	24.167697201756198	26.07176889425941	26.04468479515542	23.71584910882897
27	24.090859711807326	25.702661235957564	25.965466285505094	24.241012766730012
28	23.307156997990518	26.108674699631884	26.62565360230919	23.958514700068402
29	23.52705408833484	26.641775089871096	26.231743655450572	23.599427166343492
30	23.117320281377005	27.343183810256537	26.132236873760817	23.40725903460564
31	23.937631173103604	26.217556746396102	26.33834389166757	23.50646818883272
32	23.086367025258156	26.53805191912668	26.423614159725744	23.951966895889417
33	23.454532196594844	25.9358027483912	26.58969028390188	24.01997477111208
34	23.586976417487996	26.097712088089793	26.36428708549795	23.95102440892426
35	23.980539132306816	26.132931337840404	26.354911820423492	23.531617709429284
36	23.560636387040702	26.4732683414164	26.30679538062336	23.659299890919534
37	24.53571355936155	25.158747047907603	26.246575424007524	24.058963968723322
38	23.774184091514492	25.92756838859035	26.908895737627503	23.389351782267653
39	23.65180959977539	25.735152233966936	27.070854681903207	23.542183484354467
40	23.36157321908407	25.02962633368106	27.668093790350223	23.940706656884643
41	22.76691354864696	25.857625934860263	27.771965774825976	23.603494741666804
42	23.556568811717398	24.910674557762793	27.37041672309187	24.16233990742794
43	22.924705708445128	25.04475572970069	27.623300857216698	24.40723770463748
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	218.0
1	208.0
2	198.0
3	445.5
4	693.0
5	693.0
6	935.5
7	1178.0
8	1172.5
9	1167.0
10	1763.5
11	2360.0
12	2360.0
13	3962.5
14	5565.0
15	9090.5
16	12616.0
17	12898.0
18	13180.0
19	13180.0
20	15927.5
21	18675.0
22	21366.5
23	24058.0
24	27489.0
25	30920.0
26	30920.0
27	34610.5
28	38301.0
29	48993.5
30	59686.0
31	62954.5
32	66223.0
33	66223.0
34	72697.5
35	79172.0
36	83912.0
37	88652.0
38	101093.0
39	113534.0
40	113534.0
41	123202.0
42	132870.0
43	124450.5
44	116031.0
45	124416.5
46	132802.0
47	132802.0
48	139578.0
49	146354.0
50	151578.0
51	156802.0
52	163045.0
53	169288.0
54	169288.0
55	147491.5
56	125695.0
57	118156.0
58	110617.0
59	99662.5
60	88708.0
61	88708.0
62	82588.5
63	76469.0
64	68509.0
65	60549.0
66	53935.0
67	47321.0
68	47321.0
69	40787.0
70	34253.0
71	29625.0
72	24997.0
73	20234.0
74	15471.0
75	15471.0
76	12227.0
77	8983.0
78	7619.0
79	6255.0
80	4609.0
81	2963.0
82	2963.0
83	2235.5
84	1508.0
85	1135.5
86	763.0
87	618.5
88	474.0
89	474.0
90	377.0
91	280.0
92	170.5
93	61.0
94	43.5
95	26.0
96	26.0
97	15.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2015943.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.287115988727386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.40362875681741	41.10724324418018
2	9.48581933445764	9.35057355571053
3	2.733469610805263	4.0417450127826156
4	1.2064221065338019	2.378442651843853
5	0.6601692355343128	1.6268918841984583
6	0.4430200501280448	1.3101108357595659
7	0.2900537965166565	1.0007140578321028
8	0.21321122843032397	0.8406853236595541
9	0.18205809953133334	0.8075806801459291
>10	1.112604748132079	10.62425640667322
>50	0.13481348737780496	4.675600365245352
>100	0.11304884748384045	11.80274855814461
>500	0.01573111128940127	5.150253002955144
>1k	0.005949586962017146	5.283154420868968
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4832	0.2396893166126225	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4472	0.22183166885174826	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4197	0.20819041014552495	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3479	0.17257432377800364	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3204	0.15893306507178032	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3060	0.15179000596743064	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2832	0.14048016238554362	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2737	0.13576772755975738	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2478	0.12292014208735069	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2276	0.11290001751041573	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	2270	0.1126023900477345	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2216	0.10992374288360336	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2202	0.1092292788040138	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	2172	0.10774114149060762	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	2130	0.10565774925183898	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	2089	0.10362396159018385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	9.920915422707884E-5	0.0
13	0.0	0.0	0.0	9.920915422707884E-5	0.0
14	0.0	0.0	0.0	1.4881373134061827E-4	0.0
15	0.0	0.0	0.0	2.480228855676971E-4	0.0
16	0.0	0.0	0.0	2.480228855676971E-4	0.0
17	0.0	0.0	0.0	3.9683661690831536E-4	0.0
18	0.0	0.0	0.0	3.9683661690831536E-4	0.0
19	0.0	0.0	0.0	4.960457711353942E-4	0.0
20	0.0	0.0	0.0	6.448595024760124E-4	0.0
21	0.0	0.0	0.0	0.0010416961193843279	0.0
22	0.0	0.0	0.0	0.0012401144278384856	0.0
23	0.0	0.0	0.0	0.001884973930314498	0.0
24	0.0	0.0	0.0	0.0032739020894936017	0.0
25	0.0	0.0	0.0	0.003670738706401917	0.0
26	0.0	0.0	0.0	0.005010062288467482	0.0
27	0.0	0.0	0.0	0.01190509850724946	0.0
28	0.0	0.0	0.0	0.02723291283533314	0.0
29	0.0	0.0	0.0	0.049108531342404026	0.0
30	0.0	0.0	0.0	0.0768870945259861	0.0
31	0.0	0.0	0.0	0.16895318964871528	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1745	0.0	19.719196	1
GTCGTTA	65	0.0015804928	17.076923	34
TTGCGCG	65	0.0015804928	17.076923	18
TCTATAC	165	5.4569682E-11	16.818182	3
AACGCCG	220	0.0	15.977273	5
TAGCGAG	70	0.0025934037	15.857142	28
CTAGTAC	95	7.063897E-5	15.578948	3
TCGTTTA	155	7.21775E-9	15.5161295	30
TAGGACA	1395	0.0	15.3835125	4
CTTATAC	475	0.0	15.189472	37
TAACGCC	220	1.8189894E-12	15.136364	4
GCTTAGG	1265	0.0	15.063241	1
TAGTACG	160	1.0977601E-8	15.03125	4
ATTAGAC	175	2.240995E-9	14.8	3
ACTCGTC	150	8.110146E-8	14.8	8
AATACCG	75	0.004105833	14.8	5
TTAGGAC	1370	0.0	14.583942	3
CGAATTA	255	0.0	14.509805	15
CTTAGGA	1325	0.0	14.381132	2
ATGGTCG	155	1.211447E-7	14.322581	36
>>END_MODULE