FastQCFastQC Report
Fri 10 Feb 2017
ERR1630419.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630419.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104950
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29062.7689375893282517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26532.527870414483087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15171.4454502143878036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11271.0738446879466412No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.5250119104335398No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4560.4344926155312053No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.3582658408766079No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3760.3582658408766079No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3580.34111481657932347No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.28013339685564553No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.26965221534063843No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA2410.22963315864697473No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.22486898523106244No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA2020.1924726060028585No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1990.1896141019533111No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA1900.1810385898046689No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.18008575512148642No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1860.177227251071939No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1840.17532158170557408No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.16388756550738448No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1680.1600762267746546No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1600.15245354930919486No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1530.14578370652691758No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1530.14578370652691758No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1460.1391138637446403No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1450.13816102906145783No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1420.13530252501191042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1320.12577417818008574No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.12577417818008574No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA1310.12482134349690327No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT1240.118151500714626No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1230.11719866603144354No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1230.11719866603144354No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA1150.1095759885659838No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1100.10481181515007146No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.103858980466889No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1080.10290614578370653No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1070.10195331110052405No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1060.10100047641734158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT200.001834326237.07
GGACCGT200.001834326237.06
TAGGACC200.001834326237.04
ACCATGC303.577431E-430.8333328
GCCGCCG303.577431E-430.83333223
TCCTAGC250.005473896429.62
GTTACGG250.005473896429.617
GCCGATA250.005473896429.62
CGGTGTG250.005473896429.630
CGAATCA250.005473896429.617
TTACGGC250.005473896429.618
CGTTTAC250.005473896429.628
CCGTTTA250.005473896429.627
ACGGTGT250.005473896429.629
AGGACCG358.815583E-426.428575
CTAGGCA358.815583E-426.428574
CCTTAGG400.001919580723.1250021
CATGCTT400.001919580723.12500210
CAGCATG400.001919580723.12500236
TACTGGT400.001919580723.1250022
Produced by FastQC (version 0.11.5)