FastQCFastQC Report
Fri 10 Feb 2017
ERR1630418.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630418.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64790
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8331.2856922364562433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7471.1529557030405928No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4340.6698564593301436No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.396666152183979No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1610.24849513813860166No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1560.24077789782373824No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1550.23923444976076555No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT1460.22534341719401144No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1410.217626176879148No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1360.2099089365642846No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1190.18367031949374904No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.17440963111591296No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1080.16669239080104953No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1060.16360549467510418No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1050.1620620466121315No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1040.16051859854915881No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1020.15743170242321344No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC990.15280135823429541No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.14817101404537736No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA960.14817101404537736No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA930.14354066985645933No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC920.14199722179348667No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC910.14045377373051396No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC900.1389103256675413No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA820.12656274116375985No Hit
CACACACACTGATTCAGGCTCTGGGCTCCTCCCCGTTCGCTCG820.12656274116375985No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG780.12038894891186912No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC780.12038894891186912No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG750.11575860472295109No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT700.10804136440808766No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC700.10804136440808766No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA690.10649791634511499No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT680.1049544682821423No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT680.1049544682821423No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA660.10186757215619693No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTT660.10186757215619693No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC650.10032412409322426No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT650.10032412409322426No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.10032412409322426No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG650.10032412409322426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTACT200.001829139537.03
CAATCAT250.00545859529.624
GAGCATA250.00545859529.624
AGCATAA250.00545859529.625
CAGGTTG250.00545859529.615
GCATAAA250.00545859529.626
CATAAAC250.00545859529.627
GGATGAG500.00696517218.520
CTTATTG609.109146E-418.528
GAACAGT852.6594551E-517.4117641
AACAGTG852.6594551E-517.4117642
TTATTGA650.00155827517.07692329
CGGTGAT650.00155827517.07692314
GATTCCT650.00155827517.07692318
CTCTATG1005.7075104E-616.6500021
TTGCGTT803.3213297E-416.18754
GGTGATT700.002557243515.85714315
GGGCAGT700.002557243515.8571437
GTACACG700.002557243515.8571434
AGTCGGT700.002557243515.85714311
Produced by FastQC (version 0.11.5)