##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630418.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64790
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21463188763698	31.0	31.0	34.0	28.0	34.0
2	31.41801203889489	31.0	31.0	34.0	28.0	34.0
3	31.420095693779903	31.0	31.0	34.0	28.0	34.0
4	35.22816792714925	37.0	35.0	37.0	32.0	37.0
5	35.13661058805371	37.0	35.0	37.0	32.0	37.0
6	35.22484951381386	37.0	35.0	37.0	32.0	37.0
7	35.24965272418583	37.0	35.0	37.0	32.0	37.0
8	35.29418120080259	37.0	35.0	37.0	32.0	37.0
9	36.848989041518756	39.0	37.0	39.0	33.0	39.0
10	36.703457323661056	38.0	35.0	39.0	32.0	39.0
11	36.81353603951227	38.0	37.0	39.0	33.0	39.0
12	36.713798425682974	38.0	35.0	39.0	32.0	39.0
13	36.76880691464732	38.0	37.0	39.0	32.0	39.0
14	37.7001543448063	39.0	37.0	40.0	32.0	41.0
15	37.70350362710295	39.0	37.0	40.0	32.0	41.0
16	37.52457169316253	39.0	36.0	40.0	32.0	41.0
17	37.57399290013891	39.0	37.0	40.0	32.0	41.0
18	37.59177342182436	39.0	37.0	40.0	32.0	41.0
19	37.62324432782837	39.0	37.0	40.0	32.0	41.0
20	37.61646859083192	39.0	37.0	40.0	32.0	41.0
21	37.56453156351289	39.0	37.0	40.0	32.0	41.0
22	37.50021608272882	39.0	36.0	40.0	32.0	41.0
23	37.474347893193396	39.0	36.0	40.0	32.0	41.0
24	37.392838400987806	39.0	36.0	40.0	32.0	41.0
25	37.31632968050625	39.0	36.0	40.0	32.0	41.0
26	37.041966352832226	39.0	36.0	40.0	31.0	41.0
27	36.84601018675721	39.0	36.0	40.0	31.0	41.0
28	36.76323506713999	39.0	35.0	40.0	30.0	41.0
29	36.688424139527704	39.0	35.0	40.0	30.0	41.0
30	36.48456551937027	38.0	35.0	40.0	30.0	41.0
31	36.339990739311624	38.0	35.0	40.0	30.0	41.0
32	36.20584966815866	38.0	35.0	40.0	30.0	41.0
33	36.08859391881463	38.0	35.0	40.0	30.0	41.0
34	35.98149405772496	38.0	35.0	40.0	30.0	41.0
35	35.79760765550239	38.0	35.0	40.0	29.0	41.0
36	35.58962802901682	38.0	34.0	40.0	28.0	41.0
37	35.49572464886557	38.0	34.0	40.0	27.0	41.0
38	35.26981015588825	38.0	34.0	40.0	26.0	41.0
39	35.09004475999382	38.0	34.0	40.0	26.0	41.0
40	34.876369810155886	38.0	33.0	40.0	25.0	41.0
41	34.68161753357	38.0	33.0	40.0	24.0	41.0
42	34.5051396820497	38.0	33.0	40.0	24.0	41.0
43	33.1891495601173	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	6.0
18	3.0
19	7.0
20	27.0
21	36.0
22	85.0
23	143.0
24	239.0
25	350.0
26	538.0
27	696.0
28	1017.0
29	1300.0
30	1698.0
31	2087.0
32	2588.0
33	3234.0
34	4258.0
35	5414.0
36	7601.0
37	11301.0
38	15107.0
39	7053.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80907547461028	20.342645469979935	14.235221484797037	22.61305757061275
2	20.80722333693471	22.98965889797808	32.49421206976385	23.708905695323352
3	22.531254823275194	23.688840870504706	30.882852292020374	22.897052014199723
4	15.71075783299892	18.701960179039975	33.688840870504706	31.8984411174564
5	14.872665534804755	37.244945207593766	32.969594073159435	14.912795184442043
6	31.007871585121162	33.72125327982714	17.643154807840716	17.627720327210987
7	26.545763235067138	32.02654730668313	20.578793023614754	20.848896434634977
8	28.578484334002162	31.77342182435561	20.572619231362864	19.075474610279365
9	26.855996295724648	13.094613366260225	21.35206050316407	38.69732983485106
10	21.435406698564595	25.97468745176725	29.07547461027936	23.514431239388795
11	34.457478005865106	23.215002315172097	19.039975304830993	23.287544374131812
12	20.56953233523692	27.650872048155577	28.2204043833925	23.559191233215003
13	32.988115449915114	18.674178113906468	25.866646087359165	22.471060348819265
14	22.569841024849513	22.444821731748725	27.090600401296495	27.89473684210526
15	30.202191696249418	24.68590831918506	22.907856150640534	22.204043833924988
16	22.78592375366569	25.064053094613364	26.971754900447596	25.178268251273344
17	21.674641148325357	27.63543756752585	25.85584195091835	24.834079333230434
18	22.068220404383393	23.19339404229048	29.035344960642075	25.703040592684058
19	22.139219015280137	26.470134279981476	28.862478777589136	22.52816792714925
20	24.02376910016978	22.53434171940114	31.344343262849208	22.097545917579872
21	25.351134434326283	23.637907084426608	28.393270566445437	22.617687914801667
22	25.11807377681741	24.267633894119463	27.527396203117764	23.086896125945362
23	24.824818644852602	24.344806297268097	28.194165766321966	22.63620929155734
24	23.738231208519835	24.38030560271647	28.12934094767711	23.75212224108659
25	24.20743941966353	25.017749652724184	28.512116067294336	22.26269486031795
26	25.067139990739314	24.221330452230283	27.397746565828058	23.313782991202345
27	23.827751196172247	24.71060348819262	28.64639604877296	22.81524926686217
28	22.81524926686217	26.058033647167772	28.1185368112363	23.008180274733757
29	23.34619540052477	25.24926686217009	27.87930236147554	23.525235375829602
30	22.409322426300353	27.079796264855688	29.986108967433246	20.52477234141071
31	24.27843803056027	24.92205587281988	27.69563204198179	23.103874054638062
32	21.30730050933786	25.91294952924834	28.533724340175954	24.246025621237845
33	22.16082728816175	24.878839327056646	30.25621237845347	22.704121006328137
34	21.477079796264857	25.19524617996605	28.400987806760302	24.926686217008797
35	22.713381694705973	26.953233523691928	28.25127334465195	22.082111436950147
36	22.028090754746103	26.788084580953853	28.161753356999537	23.02207130730051
37	22.4633431085044	27.12146936255595	27.740392035807993	22.674795493131654
38	22.083654885013118	26.102793640993983	28.084580953850903	23.728970520142
39	22.11915419046149	24.736842105263158	29.783917271183824	23.360086433091524
40	21.901528013582343	25.749344034573234	29.334773884858773	23.014354066985646
41	21.322734989967586	24.693625559499925	30.125019293100785	23.858620157431705
42	20.906004012964964	25.135051705510108	30.094150331841334	23.864793949683595
43	20.9322426300355	25.95925297113752	29.523074548541437	23.58542985028554
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	9.5
2	17.0
3	36.5
4	56.0
5	56.0
6	95.5
7	135.0
8	135.5
9	136.0
10	200.0
11	264.0
12	264.0
13	477.0
14	690.0
15	1108.5
16	1527.0
17	1265.0
18	1003.0
19	1003.0
20	1208.5
21	1414.0
22	951.0
23	488.0
24	521.5
25	555.0
26	555.0
27	588.5
28	622.0
29	680.0
30	738.0
31	811.5
32	885.0
33	885.0
34	1135.0
35	1385.0
36	1422.0
37	1459.0
38	1764.0
39	2069.0
40	2069.0
41	2443.0
42	2817.0
43	3539.5
44	4262.0
45	5083.5
46	5905.0
47	5905.0
48	6966.5
49	8028.0
50	7229.5
51	6431.0
52	6082.5
53	5734.0
54	5734.0
55	5517.0
56	5300.0
57	4802.0
58	4304.0
59	3648.0
60	2992.0
61	2992.0
62	2536.5
63	2081.0
64	1686.0
65	1291.0
66	1085.5
67	880.0
68	880.0
69	792.5
70	705.0
71	522.0
72	339.0
73	241.0
74	143.0
75	143.0
76	108.5
77	74.0
78	53.0
79	32.0
80	23.5
81	15.0
82	15.0
83	11.5
84	8.0
85	5.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	64790.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.97823738231209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.93253080389499	37.68019756135206
2	10.78057542621246	10.560271646859084
3	3.989537705227996	5.862015743170242
4	2.18699776258154	4.284611822812162
5	1.301484259288438	3.1872202500385862
6	0.8981186777172029	2.6392961876832843
7	0.6901332997195349	2.36610588053712
8	0.4884505089339174	1.9138755980861244
9	0.3813065263290581	1.6808149405772497
>10	2.1491822393092366	18.91186911560426
>50	0.14811079948318784	4.858774502237999
>100	0.0472694040903791	3.616298811544991
>500	0.0063025872120505465	2.438647939496836
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	833	1.2856922364562433	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	747	1.1529557030405928	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	434	0.6698564593301436	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	257	0.396666152183979	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	161	0.24849513813860166	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	156	0.24077789782373824	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	155	0.23923444976076555	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	146	0.22534341719401144	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	141	0.217626176879148	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	136	0.2099089365642846	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	119	0.18367031949374904	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	113	0.17440963111591296	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	108	0.16669239080104953	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	106	0.16360549467510418	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	105	0.1620620466121315	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	104	0.16051859854915881	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	102	0.15743170242321344	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	99	0.15280135823429541	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96	0.14817101404537736	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	96	0.14817101404537736	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	93	0.14354066985645933	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	92	0.14199722179348667	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	91	0.14045377373051396	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	90	0.1389103256675413	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	82	0.12656274116375985	No Hit
CACACACACTGATTCAGGCTCTGGGCTCCTCCCCGTTCGCTCG	82	0.12656274116375985	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	78	0.12038894891186912	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	78	0.12038894891186912	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	75	0.11575860472295109	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	70	0.10804136440808766	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	70	0.10804136440808766	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	69	0.10649791634511499	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	68	0.1049544682821423	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	68	0.1049544682821423	No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA	66	0.10186757215619693	No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTT	66	0.10186757215619693	No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC	65	0.10032412409322426	No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT	65	0.10032412409322426	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65	0.10032412409322426	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	65	0.10032412409322426	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0015434480629726809	0.0
17	0.0	0.0	0.0	0.007717240314863405	0.0
18	0.0	0.0	0.0	0.007717240314863405	0.0
19	0.0	0.0	0.0	0.007717240314863405	0.0
20	0.0	0.0	0.0	0.007717240314863405	0.0
21	0.0	0.0	0.0	0.01543448062972681	0.0
22	0.0	0.0	0.0	0.024695169007562894	0.0
23	0.0	0.0	0.0	0.03395585738539898	0.0
24	0.0	0.0	0.0	0.055564130267016516	0.0
25	0.0	0.0	0.0	0.05865102639296188	0.0
26	0.0	0.0	0.0	0.06791171477079797	0.0
27	0.0	0.0	0.0	0.08180274733755209	0.0
28	0.0	0.0	0.0	0.09260688377836086	0.0
29	0.0	0.0	0.0	0.14199722179348664	0.0
30	0.0	0.0	0.0	0.20836548850131192	0.0
31	0.0	0.0	0.0	0.41673097700262385	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTACT	20	0.0018291395	37.0	3
CAATCAT	25	0.005458595	29.6	24
GAGCATA	25	0.005458595	29.6	24
AGCATAA	25	0.005458595	29.6	25
CAGGTTG	25	0.005458595	29.6	15
GCATAAA	25	0.005458595	29.6	26
CATAAAC	25	0.005458595	29.6	27
GGATGAG	50	0.006965172	18.5	20
CTTATTG	60	9.109146E-4	18.5	28
GAACAGT	85	2.6594551E-5	17.411764	1
AACAGTG	85	2.6594551E-5	17.411764	2
TTATTGA	65	0.001558275	17.076923	29
CGGTGAT	65	0.001558275	17.076923	14
GATTCCT	65	0.001558275	17.076923	18
CTCTATG	100	5.7075104E-6	16.650002	1
TTGCGTT	80	3.3213297E-4	16.1875	4
GGTGATT	70	0.0025572435	15.857143	15
GGGCAGT	70	0.0025572435	15.857143	7
GTACACG	70	0.0025572435	15.857143	4
AGTCGGT	70	0.0025572435	15.857143	11
>>END_MODULE