FastQCFastQC Report
Fri 10 Feb 2017
ERR1630417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1552986
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG63060.4060564615521325No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG46720.30083980151785017No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA40100.2582122440253808No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT37550.24179226342027552No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT36420.2345159582893857No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC32580.20978939926052131No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG26760.17231320823239876No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC25800.16613156847518265No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC25420.16368466940461796No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT24550.15808255837464086No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA23160.14913205914283836No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC22800.14681394423388233No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC22510.14494657389055662No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA22500.14488218180975232No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA22380.14410947684010028No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC22250.14327237978964397No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA21380.13767026875966687No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG21010.13528776176990648No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC20810.13399992015381978No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA20720.1334203914265808No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC20670.1330984310225591No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC20370.13116666859842907No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC20120.12955686657832075No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA19790.1274319279117777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.12221616936662662No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT18750.12073515150812693No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG18250.1175155474679102No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG18000.11590574544780184No Hit
GATCAGGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGA17800.11461790383171515No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT17700.11397398302367182No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC17440.11229978892275912No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT17370.11184904435712878No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA17340.11165586811471577No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG17000.10946653736736842No Hit
TTCCCAGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAG16410.1056674045999127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15730.10128874310521795No Hit
CTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTT15700.10109556686280494No Hit
ACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTC15680.10096678270119627No Hit
CTGGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGAC15620.10058043021637025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC454.009449E-628.7777793
TTAATAC1550.020.2903233
TCTTATA7250.018.62758437
GACGGAC2300.018.57
TATACGG500.00703596818.52
AAGACGG2750.018.1636375
ACGGACC2450.018.1224488
ATACCGT1603.45608E-1117.343756
GCTTAGG14650.017.300341
GGATATA2900.017.2241382
CGGCCTT9100.017.07692324
CAAGACG3050.016.9836064
CTTATAC3300.016.81818237
TACCGTC1352.2208042E-816.4444437
TAGAACA15350.016.2703594
TCCTCGG9800.016.23469420
TTCCTCG9700.016.2113419
TATACAA3550.016.154935
CGGACCA2750.016.1454549
GTAGAAC14950.016.0869563
Produced by FastQC (version 0.11.5)