##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630417.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1552986
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.321969418912985	31.0	31.0	34.0	30.0	34.0
2	31.54282395333892	31.0	31.0	34.0	30.0	34.0
3	31.66452241037588	31.0	31.0	34.0	30.0	34.0
4	35.422991578803675	37.0	35.0	37.0	33.0	37.0
5	35.25778854413369	37.0	35.0	37.0	33.0	37.0
6	35.33326700949011	37.0	35.0	37.0	33.0	37.0
7	35.31804150198392	37.0	35.0	37.0	32.0	37.0
8	35.279637421071406	37.0	35.0	37.0	32.0	37.0
9	36.930365759897384	39.0	37.0	39.0	33.0	39.0
10	36.77919955492194	38.0	37.0	39.0	33.0	39.0
11	36.88245096865007	39.0	37.0	39.0	33.0	39.0
12	36.73948380732344	38.0	37.0	39.0	32.0	39.0
13	36.77644164210109	39.0	37.0	39.0	33.0	39.0
14	37.87992615516173	39.0	37.0	41.0	33.0	41.0
15	37.86729114106631	39.0	37.0	41.0	33.0	41.0
16	37.82350967748582	39.0	37.0	41.0	33.0	41.0
17	37.77517054242601	39.0	37.0	40.0	33.0	41.0
18	37.70891817440724	39.0	37.0	40.0	33.0	41.0
19	37.74174139367644	39.0	37.0	40.0	32.0	41.0
20	37.674189593467034	39.0	37.0	40.0	32.0	41.0
21	37.61967847746213	39.0	37.0	40.0	32.0	41.0
22	37.61156056783513	39.0	37.0	40.0	32.0	41.0
23	37.543885134830575	39.0	37.0	40.0	32.0	41.0
24	37.53587411605771	39.0	37.0	40.0	32.0	41.0
25	37.49190784720532	39.0	37.0	40.0	32.0	41.0
26	37.289381874659526	39.0	36.0	40.0	32.0	41.0
27	37.16779095239751	39.0	36.0	40.0	31.0	41.0
28	37.086770904567075	39.0	36.0	40.0	31.0	41.0
29	37.020376873970534	39.0	36.0	40.0	31.0	41.0
30	36.97301392285571	39.0	36.0	40.0	31.0	41.0
31	36.85120406751896	39.0	36.0	40.0	30.0	41.0
32	36.734219754717685	39.0	36.0	40.0	30.0	41.0
33	36.68697077758589	39.0	36.0	40.0	30.0	41.0
34	36.59141486143468	39.0	35.0	40.0	30.0	41.0
35	36.499741143835166	38.0	35.0	40.0	30.0	41.0
36	36.39638605885694	38.0	35.0	40.0	30.0	41.0
37	36.34999349639984	38.0	35.0	40.0	30.0	41.0
38	36.213363159745164	38.0	35.0	40.0	30.0	41.0
39	36.11823867053534	38.0	35.0	40.0	29.0	41.0
40	36.040565723065114	38.0	35.0	40.0	29.0	41.0
41	35.91775392695105	38.0	35.0	40.0	29.0	41.0
42	35.892393749847066	38.0	35.0	40.0	28.0	41.0
43	34.591097408476315	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	0.0
13	3.0
14	6.0
15	7.0
16	18.0
17	62.0
18	121.0
19	244.0
20	494.0
21	876.0
22	1535.0
23	2636.0
24	4221.0
25	6536.0
26	9536.0
27	13528.0
28	19029.0
29	25166.0
30	33690.0
31	42941.0
32	55534.0
33	70732.0
34	91963.0
35	121532.0
36	169332.0
37	254189.0
38	403239.0
39	225812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.399536119449884	19.274223978838187	13.179449138627136	26.146790763084795
2	18.449361423734665	21.76420135146099	34.90520841784794	24.881228806956404
3	19.994835755119492	22.946311170866963	30.92223625969584	26.13661681431771
4	15.777476422839614	15.823452368533907	33.50184740879828	34.897223799828204
5	14.270830516179798	37.56891562448084	34.02026805135397	14.139985807985392
6	35.41551565822229	34.685438246062745	15.432592438051598	14.466453657663367
7	29.421192464066	31.047221288537052	20.545259261834943	18.98632698556201
8	26.311312529539865	35.495812582985295	19.820655176543767	18.37221971093107
9	26.440032299067735	14.971351963250152	18.574346452575877	40.01426928510624
10	16.587464407277334	28.291175838030735	32.428624598032435	22.692735156659495
11	35.941663350474506	21.765875545561904	21.301866211285873	20.99059489267772
12	21.39954899786605	25.96745881804472	28.097870811456122	24.53512137263311
13	30.05010991728193	19.35838442844945	25.519611896050577	25.07189375821804
14	23.032725343306378	20.54480851726931	23.856879585521053	32.56558655390325
15	25.608601751722166	28.182546397713825	21.813590077437915	24.395261773126094
16	25.85599612617242	26.59953148322007	22.674190237387844	24.87028215321967
17	24.710396616582507	26.802173361511304	23.94490355998058	24.542526461925608
18	23.122037159381993	25.48213570502245	26.223224162999536	25.17260297259602
19	25.545626296695527	25.659085143072762	25.51420296126301	23.281085598968694
20	25.23203686317842	24.548708101682823	26.171195361709636	24.048059673429123
21	24.73821399548998	26.060891727291814	24.361842283188643	24.839051994029564
22	24.92450028525692	25.065905294703235	24.576396696428688	25.433197723611162
23	23.745481285729557	26.141510612458834	24.96596878529491	25.147039316516697
24	25.899331996553737	25.887870206170565	24.583415433236357	23.62938236403934
25	25.479238061386262	25.020895230221008	25.13654340734559	24.363323301047142
26	25.736484424199574	25.27588787020617	24.49088401312053	24.496743692473725
27	25.353222759252176	24.636796468223153	24.74941821754993	25.26056255497474
28	24.233637650307216	25.143755320395677	25.651422485457047	24.97118454384006
29	24.29403742210168	26.041509710969706	24.865066394674518	24.799386472254096
30	23.794032914656025	27.082794049656595	24.817223078636896	24.305949957050483
31	25.30177348668951	25.377884926200235	25.006085051636013	24.31425653547424
32	23.833698436431494	25.693856866707105	25.217097900431813	25.25534679642959
33	24.30549921248485	24.95083664630589	25.32920451311216	25.4144596280971
34	24.564097808995058	25.133903332032613	25.493597495405623	24.808401363566706
35	25.20402630802853	24.918511821742115	25.260369378732328	24.617092491497026
36	24.31586633749435	25.96803834677196	25.291857106245647	24.424238209488045
37	25.952841815702136	24.20208553071309	24.934416665700784	24.910655987883988
38	24.64967488438402	24.990437776000558	26.239129006958205	24.120758332657218
39	24.87433885431034	24.409943167549482	26.20525877245513	24.51045920568505
40	24.241557876246148	23.97355803593851	26.98305071649068	24.801833371324662
41	23.560933582144333	24.964874119921234	27.120270240684718	24.35392205724971
42	24.891595931965902	23.708906583832697	26.536427244031817	24.863070240169584
43	23.59937565438452	24.08057767423531	26.963797484330186	25.35624918704998
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	26.0
2	34.0
3	110.0
4	186.0
5	186.0
6	279.5
7	373.0
8	352.0
9	331.0
10	487.5
11	644.0
12	644.0
13	1124.0
14	1604.0
15	3194.5
16	4785.0
17	4614.0
18	4443.0
19	4443.0
20	5270.5
21	6098.0
22	7103.0
23	8108.0
24	9743.5
25	11379.0
26	11379.0
27	13607.0
28	15835.0
29	24763.5
30	33692.0
31	35612.0
32	37532.0
33	37532.0
34	43510.5
35	49489.0
36	53369.0
37	57249.0
38	73422.0
39	89595.0
40	89595.0
41	98624.5
42	107654.0
43	100154.0
44	92654.0
45	103692.5
46	114731.0
47	114731.0
48	124163.5
49	133596.0
50	138415.5
51	143235.0
52	153624.0
53	164013.0
54	164013.0
55	135954.0
56	107895.0
57	99041.5
58	90188.0
59	80286.5
60	70385.0
61	70385.0
62	64839.0
63	59293.0
64	52271.0
65	45249.0
66	39457.0
67	33665.0
68	33665.0
69	28877.0
70	24089.0
71	21222.0
72	18355.0
73	14802.0
74	11249.0
75	11249.0
76	8738.5
77	6228.0
78	5247.5
79	4267.0
80	3254.5
81	2242.0
82	2242.0
83	1672.5
84	1103.0
85	886.5
86	670.0
87	545.5
88	421.0
89	421.0
90	366.5
91	312.0
92	189.0
93	66.0
94	45.5
95	25.0
96	25.0
97	15.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1552986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.86735221470958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43596824428599	35.723803019179975
2	10.744974548929804	9.427071661519877
3	3.22485242027841	4.24397210882435
4	1.3244066630147893	2.3239285424791176
5	0.7120293773986726	1.561742174278397
6	0.473052762386217	1.2450943286242502
7	0.3201645029892528	0.9831338313493895
8	0.23462267739185813	0.8233820501365455
9	0.16043119495404423	0.6333922561748218
>10	1.0481281876293984	8.923119766465994
>50	0.139029177093449	4.30373303946365
>100	0.14731445284877423	13.915104820757982
>500	0.024429753246624202	7.349064194738165
>1k	0.010448870364519991	8.136353258433587
>5k	1.4716718823267594E-4	0.4071049475738683
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	6306	0.4060564615521325	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	4672	0.30083980151785017	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	4010	0.2582122440253808	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	3755	0.24179226342027552	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3642	0.2345159582893857	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3258	0.20978939926052131	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	2676	0.17231320823239876	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	2580	0.16613156847518265	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	2542	0.16368466940461796	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	2455	0.15808255837464086	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	2316	0.14913205914283836	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	2280	0.14681394423388233	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2251	0.14494657389055662	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	2250	0.14488218180975232	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	2238	0.14410947684010028	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	2225	0.14327237978964397	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	2138	0.13767026875966687	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	2101	0.13528776176990648	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2081	0.13399992015381978	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2072	0.1334203914265808	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	2067	0.1330984310225591	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	2037	0.13116666859842907	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	2012	0.12955686657832075	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	1979	0.1274319279117777	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1898	0.12221616936662662	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	1875	0.12073515150812693	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1825	0.1175155474679102	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	1800	0.11590574544780184	No Hit
GATCAGGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGA	1780	0.11461790383171515	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1770	0.11397398302367182	No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC	1744	0.11229978892275912	No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT	1737	0.11184904435712878	No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA	1734	0.11165586811471577	No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG	1700	0.10946653736736842	No Hit
TTCCCAGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAG	1641	0.1056674045999127	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1573	0.10128874310521795	No Hit
CTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTT	1570	0.10109556686280494	No Hit
ACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTC	1568	0.10096678270119627	No Hit
CTGGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGAC	1562	0.10058043021637025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	6.439208080433436E-5	0.0
15	0.0	0.0	0.0	1.2878416160866873E-4	0.0
16	0.0	0.0	0.0	2.5756832321733745E-4	0.0
17	6.439208080433436E-5	0.0	0.0	4.507445656303405E-4	0.0
18	6.439208080433436E-5	0.0	0.0	4.507445656303405E-4	0.0
19	6.439208080433436E-5	0.0	0.0	4.507445656303405E-4	0.0
20	6.439208080433436E-5	0.0	0.0	6.439208080433436E-4	0.0
21	6.439208080433436E-5	0.0	0.0	9.658812120650154E-4	0.0
22	6.439208080433436E-5	0.0	0.0	0.001609802020108359	0.0
23	6.439208080433436E-5	0.0	0.0	0.0018673703433256964	0.0
24	6.439208080433436E-5	0.0	0.0	0.0027688594745863774	0.0
25	6.439208080433436E-5	0.0	0.0	0.0032839961210210525	0.0
26	6.439208080433436E-5	0.0	0.0	0.0037347406866513927	0.0
27	6.439208080433436E-5	0.0	0.0	0.0065036001612377705	0.0
28	6.439208080433436E-5	0.0	0.0	0.016870725170735602	0.0
29	6.439208080433436E-5	0.0	0.0	0.03432097906871021	0.0
30	6.439208080433436E-5	0.0	0.0	0.06200957381457399	0.0
31	6.439208080433436E-5	0.0	0.0	0.17308591320205075	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	45	4.009449E-6	28.777779	3
TTAATAC	155	0.0	20.290323	3
TCTTATA	725	0.0	18.627584	37
GACGGAC	230	0.0	18.5	7
TATACGG	50	0.007035968	18.5	2
AAGACGG	275	0.0	18.163637	5
ACGGACC	245	0.0	18.122448	8
ATACCGT	160	3.45608E-11	17.34375	6
GCTTAGG	1465	0.0	17.30034	1
GGATATA	290	0.0	17.224138	2
CGGCCTT	910	0.0	17.076923	24
CAAGACG	305	0.0	16.983606	4
CTTATAC	330	0.0	16.818182	37
TACCGTC	135	2.2208042E-8	16.444443	7
TAGAACA	1535	0.0	16.270359	4
TCCTCGG	980	0.0	16.234694	20
TTCCTCG	970	0.0	16.21134	19
TATACAA	355	0.0	16.15493	5
CGGACCA	275	0.0	16.145454	9
GTAGAAC	1495	0.0	16.086956	3
>>END_MODULE