FastQCFastQC Report
Fri 10 Feb 2017
ERR1630416.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630416.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126325
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC3940.3118939244013457No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT2610.206609934692262No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA2460.1947358005145458No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG2220.1757371858301999No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG2180.17257075004947556No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA2110.167029487433208No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG1980.15673857114585396No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG1810.14328121907777558No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG1580.12507421333861074No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA1430.11320007916089453No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG1380.10924203443498913No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG1370.10845042548980803No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC1360.10765881654462696No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA1270.10053433603799723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTGA200.001835740537.01
TTAGAGT200.001835740537.04
GCTTTAT507.223207E-933.31
AAGCATT303.581525E-430.83333222
TCGCCCA250.00547806829.59999812
CCTACTC250.00547806829.59999830
ATAGAGA250.00547806829.5999986
TCTAGGG250.00547806829.5999983
GAGAATA250.00547806829.59999818
TTTGCCA250.00547806829.59999827
ATAACGC250.00547806829.5999983
TGTCGCC250.00547806829.59999810
AAAGCAT358.825575E-426.4285721
CAGGACA400.001921736523.1254
CATTTGC400.001921736523.12525
GGCCAAA753.6865094E-722.212
CTTTATT753.6865094E-722.22
GAGCGAA502.6836945E-422.19999916
GGTATCA855.0893505E-821.7647061
TTATTGA806.8597546E-720.81254
Produced by FastQC (version 0.11.5)