##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630416.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	126325
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38055808430635	31.0	31.0	34.0	30.0	34.0
2	31.59925588759153	31.0	31.0	34.0	30.0	34.0
3	31.736212151197307	31.0	31.0	34.0	30.0	34.0
4	35.45713833366317	37.0	35.0	37.0	33.0	37.0
5	35.34938452404512	37.0	35.0	37.0	33.0	37.0
6	35.410377993271325	37.0	35.0	37.0	33.0	37.0
7	35.401369483475165	37.0	35.0	37.0	33.0	37.0
8	35.36658618642391	37.0	35.0	37.0	33.0	37.0
9	37.00769443894716	39.0	37.0	39.0	33.0	39.0
10	36.890116762319415	39.0	37.0	39.0	33.0	39.0
11	36.979465663962	39.0	37.0	39.0	33.0	39.0
12	36.82482485652088	39.0	37.0	39.0	33.0	39.0
13	36.870144468632496	39.0	37.0	39.0	33.0	39.0
14	37.97356026123095	40.0	37.0	41.0	33.0	41.0
15	37.94066099346922	40.0	37.0	41.0	33.0	41.0
16	37.91981792994261	40.0	37.0	41.0	33.0	41.0
17	37.864761527805264	40.0	37.0	41.0	33.0	41.0
18	37.823597862655845	40.0	37.0	41.0	33.0	41.0
19	37.815175143479124	40.0	37.0	41.0	33.0	41.0
20	37.77880071244805	39.0	37.0	41.0	33.0	41.0
21	37.70985157332278	39.0	37.0	41.0	32.0	41.0
22	37.65582426281417	39.0	37.0	41.0	32.0	41.0
23	37.62094597268949	39.0	37.0	40.0	32.0	41.0
24	37.565010884623	39.0	37.0	41.0	32.0	41.0
25	37.536631703938255	39.0	37.0	40.0	32.0	41.0
26	37.3383494953493	39.0	36.0	40.0	31.0	41.0
27	37.21346526815753	39.0	36.0	40.0	31.0	41.0
28	37.09151791015238	39.0	36.0	40.0	31.0	41.0
29	37.037324361765286	39.0	36.0	40.0	31.0	41.0
30	36.97881654462695	39.0	36.0	40.0	31.0	41.0
31	36.884045121709875	39.0	36.0	40.0	30.0	41.0
32	36.80274688303978	39.0	36.0	40.0	30.0	41.0
33	36.72713239659608	39.0	36.0	40.0	30.0	41.0
34	36.63958836334851	39.0	35.0	40.0	30.0	41.0
35	36.56470215713438	39.0	35.0	40.0	30.0	41.0
36	36.42574312289729	39.0	35.0	40.0	30.0	41.0
37	36.38726301207203	38.0	35.0	40.0	30.0	41.0
38	36.28117949732832	38.0	35.0	40.0	30.0	41.0
39	36.16643578072432	38.0	35.0	40.0	29.0	41.0
40	36.07962002770631	38.0	35.0	40.0	29.0	41.0
41	35.95440332475757	38.0	35.0	40.0	28.0	41.0
42	35.90627350089056	38.0	35.0	40.0	28.0	41.0
43	34.55681773204037	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	5.0
18	8.0
19	8.0
20	29.0
21	80.0
22	117.0
23	240.0
24	364.0
25	518.0
26	758.0
27	1099.0
28	1500.0
29	2087.0
30	2667.0
31	3542.0
32	4458.0
33	5536.0
34	7420.0
35	9803.0
36	13133.0
37	19471.0
38	32364.0
39	21117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02552938848209	18.493568177320405	12.44013457352068	26.040767860676826
2	20.761527805264198	19.921630714427074	31.329507223431623	27.9873342568771
3	21.601424896101324	20.926974074807045	28.556501088462298	28.91509994062933
4	16.253314862457945	14.3597862655848	32.83593904611122	36.55095982584603
5	16.030872748862063	35.057985355234514	33.880071244805066	15.031070651098357
6	38.80942014644766	33.27132396596081	13.481891945378981	14.437363942212547
7	31.982584603206014	28.575499703146644	19.225014842667722	20.216900850979616
8	30.321789036216106	30.905996437759747	18.552938848208985	20.21927567781516
9	27.970710469028297	13.256283396002374	17.796160696615875	40.97684543835346
10	18.56402137344152	25.610924203443496	31.344547793390063	24.480506629724914
11	39.53770037601425	20.20344349891154	18.863249554719967	21.395606570354246
12	25.01880071244805	23.886008311893924	26.140510587769644	24.954680387888384
13	34.0209776370473	17.272907183851178	22.42232337225411	26.283791806847418
14	24.608747278844252	18.73342568771027	22.309914902038393	34.347912131407085
15	27.78072432218484	26.302790421531764	19.738769048090244	26.177716208193154
16	27.783890757965562	24.18444488422719	21.326736592123492	26.70492776568375
17	26.774589352859685	24.038788838313874	22.38353453394023	26.803087274886206
18	26.07401543637443	22.79833762121512	24.266772214526025	26.860874727884426
19	27.911339798139718	22.67959627943796	23.92638036809816	25.482683554324165
20	29.052839897090838	22.19829804076786	21.996437759746684	26.752424302394616
21	28.62062141302197	22.434989115377004	22.20542252127449	26.738966950326535
22	28.409261824658614	22.590936077577677	23.03186226004354	25.967939837720166
23	27.527409459726893	22.517316445675835	22.31466455570948	27.64060953888779
24	26.924995052444096	23.101523847219475	23.56778151593113	26.4056995844053
25	27.718187215515535	22.985948941223036	22.359786265584802	26.936077577676627
26	27.746685137542055	23.122897288739363	22.474569562636056	26.65584801108253
27	27.568573124876313	22.047892341183456	22.97961606966159	27.403918464278647
28	26.95032653868989	23.156144864436968	22.371660399762515	27.521868197110628
29	26.508608747278846	23.42766673263408	23.85988521670295	26.20383930338413
30	27.32871561448644	23.109439936671283	22.546605976647534	27.015238472194735
31	26.949534929744708	24.002374826835542	22.831585196912727	26.216505046507027
32	26.324163863051652	22.830001979022363	22.999406293291113	27.84642786463487
33	25.432812190777753	23.482287749851576	23.614486443696812	27.470413615673856
34	27.863051652483673	21.599841678210964	23.68256481298239	26.854541856322978
35	26.852958638432618	22.43894716010291	23.41975064318227	27.288343558282207
36	25.592717197704335	23.58440530377993	24.13932317435187	26.68355432416386
37	26.939244013457355	22.141302196714825	24.453591925588757	26.465861864239066
38	26.507025529388486	22.83475163269345	23.73006134969325	26.928161488224816
39	25.96398179299426	21.900653077379776	25.147041361567386	26.98832376805858
40	24.976845438353454	21.387690480902435	25.76607955669899	27.86938452404512
41	24.204235107856718	21.812784484464675	26.462695428458343	27.520284979220268
42	25.015634276667324	21.127251137937858	27.22422323372254	26.63289135167227
43	24.732238274292502	20.958638432614286	26.919453789827823	27.389669503265385
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	3.5
4	5.0
5	5.0
6	6.5
7	8.0
8	12.5
9	17.0
10	15.0
11	13.0
12	13.0
13	27.5
14	42.0
15	83.5
16	125.0
17	114.0
18	103.0
19	103.0
20	154.5
21	206.0
22	239.5
23	273.0
24	372.5
25	472.0
26	472.0
27	634.5
28	797.0
29	1017.0
30	1237.0
31	1566.0
32	1895.0
33	1895.0
34	2215.5
35	2536.0
36	3040.0
37	3544.0
38	3994.5
39	4445.0
40	4445.0
41	4906.0
42	5367.0
43	5763.5
44	6160.0
45	6863.0
46	7566.0
47	7566.0
48	8358.5
49	9151.0
50	9409.5
51	9668.0
52	10088.5
53	10509.0
54	10509.0
55	10362.5
56	10216.0
57	10421.5
58	10627.0
59	10637.5
60	10648.0
61	10648.0
62	9876.5
63	9105.0
64	8336.0
65	7567.0
66	6551.0
67	5535.0
68	5535.0
69	4738.5
70	3942.0
71	3208.5
72	2475.0
73	1739.5
74	1004.0
75	1004.0
76	728.0
77	452.0
78	361.0
79	270.0
80	214.0
81	158.0
82	158.0
83	121.0
84	84.0
85	61.5
86	39.0
87	35.5
88	32.0
89	32.0
90	26.5
91	21.0
92	12.5
93	4.0
94	2.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	126325.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.0288937264991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.52328453650138	68.49396398179299
2	4.123314477581616	6.104888185236494
3	1.127067805853481	2.5030674846625764
4	0.5400087684592106	1.5990500692657827
5	0.30154945090197505	1.1161686127053236
6	0.2149341830897056	0.9546803878883832
7	0.16788391415464568	0.8699782307540075
8	0.12083364521958576	0.7156144864436967
9	0.10479378080990621	0.698199089649713
>10	0.6865061967342836	10.516524836730655
>50	0.06202080905076083	3.2083910548189194
>100	0.02780243164344451	3.2194735800514547
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	394	0.3118939244013457	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	261	0.206609934692262	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	246	0.1947358005145458	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	222	0.1757371858301999	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	218	0.17257075004947556	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	211	0.167029487433208	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	198	0.15673857114585396	No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG	181	0.14328121907777558	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	158	0.12507421333861074	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	143	0.11320007916089453	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	138	0.10924203443498913	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	137	0.10845042548980803	No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC	136	0.10765881654462696	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	127	0.10053433603799723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.001583217890362161	0.0
17	0.0	0.0	0.0	0.001583217890362161	0.0
18	0.0	0.0	0.0	0.001583217890362161	0.0
19	0.0	0.0	0.0	0.001583217890362161	0.0
20	0.0	0.0	0.0	0.001583217890362161	0.0
21	0.0	0.0	0.0	0.002374826835543242	0.0
22	0.0	0.0	0.0	0.003958044725905403	0.0
23	0.0	0.0	0.0	0.007124480506629725	0.0
24	0.0	0.0	0.0	0.009499307342172967	0.0
25	0.0	0.0	0.0	0.011082525232535128	0.0
26	0.0	0.0	0.0	0.012665743122897288	0.0
27	0.0	0.0	0.0	0.018998614684345935	0.0
28	0.0	0.0	0.0	0.04195527409459727	0.0
29	0.0	0.0	0.0	0.09420146447654859	0.0
30	7.916089451810805E-4	0.0	0.0	0.15119730852958638	0.0
31	7.916089451810805E-4	0.0	0.0	0.3823471205224619	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTGA	20	0.0018357405	37.0	1
TTAGAGT	20	0.0018357405	37.0	4
GCTTTAT	50	7.223207E-9	33.3	1
AAGCATT	30	3.581525E-4	30.833332	22
TCGCCCA	25	0.005478068	29.599998	12
CCTACTC	25	0.005478068	29.599998	30
ATAGAGA	25	0.005478068	29.599998	6
TCTAGGG	25	0.005478068	29.599998	3
GAGAATA	25	0.005478068	29.599998	18
TTTGCCA	25	0.005478068	29.599998	27
ATAACGC	25	0.005478068	29.599998	3
TGTCGCC	25	0.005478068	29.599998	10
AAAGCAT	35	8.825575E-4	26.42857	21
CAGGACA	40	0.0019217365	23.125	4
CATTTGC	40	0.0019217365	23.125	25
GGCCAAA	75	3.6865094E-7	22.2	12
CTTTATT	75	3.6865094E-7	22.2	2
GAGCGAA	50	2.6836945E-4	22.199999	16
GGTATCA	85	5.0893505E-8	21.764706	1
TTATTGA	80	6.8597546E-7	20.8125	4
>>END_MODULE