FastQCFastQC Report
Fri 10 Feb 2017
ERR1630415.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630415.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2878341
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32510.11294700662638651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32420.11263432650961093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG502.703471E-422.27
GGTATCA17700.017.0367241
GACGGAC6450.016.6356587
CAAGACG7950.015.358494
AGACGGA7150.015.2657366
ATCGTAC855.367634E-415.23529425
TTAGACT2350.014.9574474
CGGACCA7200.014.9027789
AAGACGG7950.014.8930815
TCTAGCG3750.014.80000128
GCGCAAG7250.014.7999991
TTAACGG908.27935E-414.38888835
ACGGACC7950.013.9622638
GCGAAAG7450.013.9060418
CTAGCGG3750.013.81333429
TCTTATA11450.013.410480537
CGCAAGA7950.013.2641512
TATCTAG4200.013.2142851
GTATTAA4100.013.0853661
TAGACTG4100.013.0853665
Produced by FastQC (version 0.11.5)