##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630415.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2878341
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.839831347293458	31.0	31.0	34.0	30.0	34.0
2	32.03332857364711	33.0	31.0	34.0	30.0	34.0
3	32.19120597594239	34.0	31.0	34.0	30.0	34.0
4	35.83488335815666	37.0	35.0	37.0	35.0	37.0
5	35.75099962096221	37.0	35.0	37.0	35.0	37.0
6	35.81081636956844	37.0	35.0	37.0	35.0	37.0
7	35.77776052246763	37.0	35.0	37.0	35.0	37.0
8	35.75963098187463	37.0	35.0	37.0	33.0	37.0
9	37.47185722608961	39.0	37.0	39.0	35.0	39.0
10	37.38003315104083	39.0	37.0	39.0	34.0	39.0
11	37.46396274798573	39.0	37.0	39.0	35.0	39.0
12	37.332714574124466	39.0	37.0	39.0	34.0	39.0
13	37.377280523746144	39.0	37.0	39.0	34.0	39.0
14	38.60444540796244	40.0	38.0	41.0	34.0	41.0
15	38.59828109317138	40.0	38.0	41.0	34.0	41.0
16	38.580084500064444	40.0	38.0	41.0	34.0	41.0
17	38.541598094179946	40.0	38.0	41.0	34.0	41.0
18	38.50817814845426	40.0	38.0	41.0	34.0	41.0
19	38.51875472711538	40.0	38.0	41.0	34.0	41.0
20	38.46792093084176	40.0	38.0	41.0	34.0	41.0
21	38.42667216983672	40.0	38.0	41.0	34.0	41.0
22	38.39806332884116	40.0	38.0	41.0	34.0	41.0
23	38.345405217797335	40.0	38.0	41.0	34.0	41.0
24	38.332754527694945	40.0	38.0	41.0	34.0	41.0
25	38.30753791854405	40.0	38.0	41.0	34.0	41.0
26	38.147967527127605	40.0	38.0	41.0	33.0	41.0
27	38.05765091766403	40.0	38.0	41.0	33.0	41.0
28	37.98000236942044	40.0	37.0	41.0	33.0	41.0
29	37.91524631723621	40.0	37.0	41.0	33.0	41.0
30	37.85727090709544	40.0	37.0	41.0	33.0	41.0
31	37.75789699691593	40.0	37.0	41.0	33.0	41.0
32	37.666056593016606	40.0	37.0	41.0	32.0	41.0
33	37.60756769263961	40.0	37.0	41.0	32.0	41.0
34	37.52018714947256	40.0	37.0	41.0	32.0	41.0
35	37.44570014463193	40.0	37.0	41.0	32.0	41.0
36	37.35077740962589	40.0	37.0	41.0	31.0	41.0
37	37.291045779495896	39.0	36.0	41.0	31.0	41.0
38	37.19596739927618	39.0	36.0	41.0	31.0	41.0
39	37.1058974596825	39.0	36.0	41.0	31.0	41.0
40	37.01324617201367	39.0	36.0	41.0	31.0	41.0
41	36.89345320794166	39.0	36.0	41.0	30.0	41.0
42	36.835162338305295	39.0	35.0	41.0	30.0	41.0
43	35.642657002766526	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	6.0
12	0.0
13	3.0
14	4.0
15	3.0
16	21.0
17	31.0
18	78.0
19	208.0
20	433.0
21	844.0
22	1572.0
23	2708.0
24	4486.0
25	7052.0
26	10898.0
27	16139.0
28	22873.0
29	32488.0
30	43664.0
31	58440.0
32	76614.0
33	98874.0
34	132149.0
35	179067.0
36	249291.0
37	374504.0
38	684303.0
39	881582.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91885499320615	18.475920677918285	11.813541203074966	26.791683125800592
2	18.797460064669195	20.23773416700801	33.797698048980294	27.167107719342496
3	19.64770678665245	22.266715444764884	29.36771563897398	28.71786212960869
4	14.606747428466607	15.219357261700402	34.09144364757338	36.082451662259615
5	15.243294661751335	35.80006677457605	33.926695968267836	15.029942595404783
6	35.86847423567951	34.51675809085859	15.359403211780675	14.255364461681225
7	30.447191628788943	29.90288502995302	20.769915725760082	18.880007615497956
8	28.28744057774947	33.12060662722033	19.176671561847606	19.415281233182586
9	27.041375570163506	13.893454597631067	18.669295959026396	40.39587387317903
10	17.055901298699496	26.13390838681032	32.696264966520644	24.11392534796954
11	37.46800674416269	21.01675930683682	20.857014509399686	20.658219439600796
12	22.254104013388268	24.381371074518274	29.12212277836434	24.242402133729115
13	30.95321228443746	18.947928685308653	23.98642134479549	26.112437685458396
14	23.21455310541732	19.58861024458186	24.14793799622769	33.04889865377313
15	25.845929999259994	27.0876174852111	21.120395394430332	25.946057121098576
16	25.93306352513479	25.563197689224452	23.171229538126305	25.33250924751445
17	24.60976652870525	25.534709056362676	24.577386765501377	25.278137649430697
18	24.98887379917807	24.651422468706798	25.420163906917214	24.93953982519792
19	25.345919750300606	24.777710493648947	25.05585682863844	24.820512927412004
20	25.555068006188286	24.512314558976854	24.853170628497455	25.0794468063374
21	25.9149975628322	24.505644049818976	24.569222340230016	25.01013604711881
22	25.770990997939435	24.312477222122048	24.70412643950109	25.212405340437428
23	25.287900217521138	24.3682037673785	24.94937187775875	25.394524137341612
24	25.33292615433682	24.83621641772118	24.586662942299053	25.244194485642947
25	25.6202444394184	24.27321849634911	24.880373798656937	25.22616326557555
26	25.423116996908984	24.877455450900364	24.74227341374771	24.957154138442945
27	25.462966340680275	24.363895730214036	24.685782539316918	25.487355389788767
28	25.238566243540983	24.51929774825151	24.926893651586106	25.315242356621404
29	24.963025576191285	24.832985389847835	25.14917447237836	25.05481456158252
30	24.899065121193075	25.09136339301007	24.9871714296534	25.02240005614345
31	25.53272874895643	24.763709372864437	24.601289423317112	25.102272454862018
32	24.921786543012104	24.84684754169155	24.74064052869344	25.490725386602907
33	24.789140689028855	24.52645464870215	25.203546070462117	25.480858591806875
34	25.47335426900426	24.199773411142043	24.95837011667485	25.368502203178846
35	25.386846103363013	24.497236428901232	25.37437364092719	24.741543826808567
36	25.063395893676255	24.882041425946404	24.861786702826386	25.19277597755096
37	25.13440902241951	24.428446803210598	25.258369317603442	25.178774856766452
38	24.835556315252433	24.402320642342236	25.57963076647277	25.18249227593256
39	24.817073446127473	24.257792943921515	25.563788307222808	25.361345302728207
40	24.785319043157152	24.281904055148434	25.897244280646387	25.035532621048027
41	24.282529415381983	24.255743152044875	26.23691911417028	25.224808318402857
42	24.601706330139482	24.280305912329357	26.121262213198506	24.996725544332655
43	24.35858016822885	23.65758608865315	26.33482273295624	25.649011010161754
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	86.0
1	99.5
2	113.0
3	301.5
4	490.0
5	490.0
6	667.0
7	844.0
8	780.5
9	717.0
10	1046.0
11	1375.0
12	1375.0
13	2455.0
14	3535.0
15	5520.5
16	7506.0
17	7946.0
18	8386.0
19	8386.0
20	10814.5
21	13243.0
22	14432.0
23	15621.0
24	20191.5
25	24762.0
26	24762.0
27	29844.5
28	34927.0
29	42394.5
30	49862.0
31	58598.0
32	67334.0
33	67334.0
34	78455.5
35	89577.0
36	102045.5
37	114514.0
38	127804.5
39	141095.0
40	141095.0
41	155471.5
42	169848.0
43	176874.5
44	183901.0
45	191514.5
46	199128.0
47	199128.0
48	205460.0
49	211792.0
50	214490.0
51	217188.0
52	218246.0
53	219304.0
54	219304.0
55	213786.5
56	208269.0
57	200372.0
58	192475.0
59	184544.0
60	176613.0
61	176613.0
62	162989.5
63	149366.0
64	133271.0
65	117176.0
66	102367.0
67	87558.0
68	87558.0
69	75689.0
70	63820.0
71	53968.5
72	44117.0
73	35394.5
74	26672.0
75	26672.0
76	21174.5
77	15677.0
78	12361.0
79	9045.0
80	7392.5
81	5740.0
82	5740.0
83	4278.0
84	2816.0
85	2284.0
86	1752.0
87	1443.5
88	1135.0
89	1135.0
90	952.5
91	770.0
92	452.5
93	135.0
94	88.5
95	42.0
96	42.0
97	27.0
98	12.0
99	7.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2878341.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.58098643257985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54191145852577	41.54772105815578
2	12.395854473742066	13.283642207556156
3	4.063273683097343	6.531426362576924
4	1.854054435140303	3.973682621380683
5	1.0554811336146561	2.8276860150025445
6	0.6444830945260691	2.0719223966297036
7	0.417146784403568	1.564579533686532
8	0.3145117436405366	1.3481479575112496
9	0.23879611363574085	1.1515438192382483
>10	1.295047731266874	12.797712265606433
>50	0.10856160872414586	4.051542124834971
>100	0.06621475705608716	6.706565781762253
>500	0.0037562915606096006	1.3649638834460351
>1k	9.066910663067557E-4	0.7788639726125386
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3251	0.11294700662638651	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3242	0.11263432650961093	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	3.474223519728899E-5	0.0
14	0.0	0.0	0.0	6.948447039457799E-5	0.0
15	0.0	0.0	0.0	1.3896894078915597E-4	0.0
16	0.0	0.0	0.0	2.0845341118373397E-4	0.0
17	0.0	0.0	0.0	3.474223519728899E-4	0.0
18	0.0	0.0	0.0	3.8216458717017896E-4	0.0
19	0.0	0.0	0.0	6.253602335512019E-4	0.0
20	0.0	0.0	0.0	7.990714095376469E-4	0.0
21	0.0	0.0	0.0	0.001077009291115959	0.0
22	0.0	0.0	0.0	0.0013549471726942708	0.0
23	0.0	0.0	0.0	0.0019108229358508946	0.0
24	0.0	0.0	0.0	0.0029530899917695644	0.0
25	0.0	0.0	0.0	0.0035437079901234772	0.0
26	0.0	0.0	0.0	0.0045859750460421475	0.0
27	0.0	0.0	0.0	0.009032981151295139	0.0
28	0.0	0.0	0.0	0.022582452878237846	0.0
29	0.0	0.0	0.0	0.04078738412161728	0.0
30	0.0	0.0	0.0	0.06604498911004637	0.0
31	0.0	0.0	0.0	0.15439449321675228	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	50	2.703471E-4	22.2	7
GGTATCA	1770	0.0	17.036724	1
GACGGAC	645	0.0	16.635658	7
CAAGACG	795	0.0	15.35849	4
AGACGGA	715	0.0	15.265736	6
ATCGTAC	85	5.367634E-4	15.235294	25
TTAGACT	235	0.0	14.957447	4
CGGACCA	720	0.0	14.902778	9
AAGACGG	795	0.0	14.893081	5
TCTAGCG	375	0.0	14.800001	28
GCGCAAG	725	0.0	14.799999	1
TTAACGG	90	8.27935E-4	14.388888	35
ACGGACC	795	0.0	13.962263	8
GCGAAAG	745	0.0	13.90604	18
CTAGCGG	375	0.0	13.813334	29
TCTTATA	1145	0.0	13.4104805	37
CGCAAGA	795	0.0	13.264151	2
TATCTAG	420	0.0	13.214285	1
GTATTAA	410	0.0	13.085366	1
TAGACTG	410	0.0	13.085366	5
>>END_MODULE