FastQCFastQC Report
Fri 10 Feb 2017
ERR1630414.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630414.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342836
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC82370.3515824411098344No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT53930.23019110172457655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48530.2071421132337048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39870.17017836502426972No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT34060.14537936074057253No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG31700.1353060991038212No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG29490.12587308714737183No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT29340.1252328374670698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29320.12514747084302955No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG27980.11942790703233176No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT27140.1158425088226406No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT25770.10999489507588239No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG25710.10973879520376159No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC24310.10376313152094299No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG24140.10303751521660073No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG23550.10051919980741289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC609.240718E-418.56
GGTATCA22800.017.5263181
GACAGGC22500.017.5133347
TGCATCA21450.017.50815814
CAGGACA25650.016.8771924
GCTGCAT22150.016.87133212
GCATCAG22550.016.57206215
CCAGGAC27350.016.031083
ACAGGCT24400.015.99795158
TCTAGCG1403.4779077E-815.85714228
GGACAGG26700.015.6591756
CTGCATC23950.015.52609513
ATCGTAC1201.937491E-615.41666725
ATCAAGC24000.015.41666730
GTTCGCG1201.937491E-615.41666730
AAGACGG3250.015.369235
TCGCTAA855.367103E-415.23529414
CGCGCTT1601.0981239E-815.03125112
TCCAGGA28800.015.031252
GGCCATC24950.014.97795626
Produced by FastQC (version 0.11.5)