FastQCFastQC Report
Fri 10 Feb 2017
ERR1630413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42477
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAG470.1106481154507145No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA440.10358546978364762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTCC200.001822042237.031
GTTTCCC250.00543765529.632
CCACTCC250.00543765529.633
CACTCCC250.00543765529.634
GTCAGCC250.00543765529.632
GGCAGCA450.003766341620.55555512
Produced by FastQC (version 0.11.5)