##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630413.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42477
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.66906796619347	31.0	30.0	33.0	27.0	34.0
2	30.90672599289027	31.0	31.0	34.0	27.0	34.0
3	31.028156414059374	31.0	31.0	34.0	27.0	34.0
4	34.896767662499705	35.0	35.0	37.0	32.0	37.0
5	34.3987569743626	35.0	35.0	37.0	30.0	37.0
6	34.44937260164324	35.0	35.0	37.0	30.0	37.0
7	34.47230265790898	35.0	35.0	37.0	30.0	37.0
8	34.416248793464696	35.0	35.0	37.0	30.0	37.0
9	35.97422134331521	37.0	35.0	39.0	32.0	39.0
10	35.68526496692328	37.0	35.0	39.0	30.0	39.0
11	35.88250111825223	37.0	35.0	39.0	31.0	39.0
12	35.6758951903383	37.0	35.0	39.0	30.0	39.0
13	35.79984462179532	37.0	35.0	39.0	30.0	39.0
14	36.70748875862231	38.0	36.0	40.0	31.0	41.0
15	36.610400922852364	38.0	36.0	40.0	31.0	41.0
16	36.56694210984768	38.0	36.0	40.0	31.0	41.0
17	36.47091367092779	38.0	35.0	40.0	31.0	41.0
18	36.38743319914307	38.0	35.0	40.0	31.0	41.0
19	36.35044847799986	38.0	35.0	40.0	30.0	41.0
20	36.24552110553947	38.0	35.0	40.0	30.0	41.0
21	36.194929020411045	38.0	35.0	40.0	30.0	41.0
22	36.188878687289595	38.0	35.0	40.0	30.0	41.0
23	36.02759140240601	38.0	34.0	40.0	29.0	41.0
24	36.032017327024036	38.0	34.0	40.0	30.0	41.0
25	35.926901617345855	38.0	34.0	40.0	29.0	41.0
26	35.65541351790381	38.0	34.0	40.0	28.0	41.0
27	35.52840360665772	38.0	34.0	40.0	27.0	41.0
28	35.35367375285448	38.0	34.0	40.0	27.0	41.0
29	35.25204228170539	38.0	34.0	40.0	27.0	40.0
30	35.19309273253761	38.0	34.0	40.0	27.0	40.0
31	35.03260588082963	38.0	33.0	40.0	26.0	40.0
32	34.87049462061822	38.0	33.0	40.0	26.0	40.0
33	34.79478305906726	37.0	33.0	40.0	26.0	40.0
34	34.65868587706288	37.0	33.0	39.0	25.0	40.0
35	34.55891423593945	37.0	33.0	39.0	25.0	40.0
36	34.40968053299433	37.0	33.0	39.0	25.0	40.0
37	34.341196412176004	37.0	33.0	39.0	25.0	40.0
38	34.22174353179368	37.0	32.0	39.0	24.0	40.0
39	34.03780869647103	37.0	32.0	39.0	24.0	40.0
40	33.87885208465758	37.0	32.0	39.0	24.0	40.0
41	33.73185488617369	37.0	31.0	39.0	23.0	40.0
42	33.63300138898698	36.0	31.0	39.0	23.0	40.0
43	32.23087788685642	35.0	30.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	5.0
17	8.0
18	13.0
19	21.0
20	32.0
21	69.0
22	98.0
23	198.0
24	227.0
25	353.0
26	492.0
27	732.0
28	922.0
29	1130.0
30	1405.0
31	1907.0
32	2381.0
33	3014.0
34	3899.0
35	5042.0
36	6390.0
37	7196.0
38	5668.0
39	1274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77084069025591	18.97497469218636	12.82576453139346	26.428420086164277
2	21.437483814770346	20.96664077029922	30.776655601855126	26.819219813075314
3	21.458671751771547	21.689384843562397	29.218165124655698	27.633778280010358
4	17.505944393436447	15.606092708995456	32.31160392683099	34.576358970737104
5	16.56190408927184	35.574546225015894	32.80363490830332	15.05991477740895
6	37.32608235044848	33.89363655625397	14.139416625467902	14.640864467829648
7	31.624173081903145	29.119288085316757	19.942557148574522	19.31398168420557
8	29.69136238434918	32.102078772041345	18.579466534830612	19.627092308778867
9	28.398898227275936	13.353108741201119	17.334086682204486	40.91390634931846
10	18.26400169503496	26.181227487816937	31.108599948207267	24.44617086894084
11	39.80271676436659	19.77305365256492	19.003225274854625	21.421004308213856
12	23.55627751489041	23.71871836523295	27.19824846387457	25.52675565600207
13	32.88838665630812	17.265814440756174	22.746427478400076	27.09937142453563
14	23.9918073310262	18.918473526849823	22.958306848412082	34.1314122937119
15	27.711467382348093	25.806907267462392	19.69065611978247	26.79096923040704
16	27.9445346893613	24.47206723638675	20.938390187630954	26.645007886621
17	26.27068766626645	24.3896697036043	23.130164559644044	26.209478070485204
18	26.240082868375826	22.915930974409683	24.02005791369447	26.823928243520022
19	27.42896155566542	23.763448454457706	23.16783200320173	25.639757986675143
20	27.363043529439462	22.82882501118252	22.925347835299103	26.882783624078915
21	27.669091508345694	23.177248864091155	22.325022953598417	26.828636673964734
22	27.991618993808416	22.82882501118252	22.574569767168114	26.60498622784095
23	27.085246133201498	22.513360171386868	23.26435482731831	27.13703886809332
24	27.09937142453563	23.440920968994984	22.558090260611625	26.90161734585776
25	27.63613249523271	22.687572097841187	23.113685053087554	26.56261035383855
26	27.497233797113736	23.89057607646491	22.576923982390472	26.03526614403089
27	27.506650658003156	23.582173882336324	22.48040115827389	26.430774301386634
28	27.221790616098122	23.25022953598418	22.92299362007675	26.60498622784095
29	26.626174164842148	23.516255856110366	23.447983614662053	26.409586364385433
30	26.084704663700354	24.592132212726888	23.076017609529863	26.24714551404289
31	27.44544106222191	23.5515690844457	22.39800362549144	26.60498622784095
32	26.96047272641665	23.07366339430751	22.69934317395296	27.266520705322883
33	26.67561268451162	23.269063257763026	23.13722720531111	26.918096852414248
34	27.009911246086116	22.901805683075548	22.842950302516655	27.245332768321678
35	26.776843939072908	22.708760034842385	23.728135226122372	26.78626079996233
36	26.73446806507051	24.00357840713798	22.92299362007675	26.338959907714766
37	27.466628999223108	22.89003460696377	23.365586081879606	26.277750311933517
38	26.329543046825343	23.25022953598418	24.005932622360337	26.414294794830145
39	26.600277797396238	22.494526449608024	24.180144548814653	26.72505120418109
40	25.514984579890292	22.83588765684959	25.326647362101845	26.32248040115827
41	25.331355792546557	22.656967299950562	25.37608588177131	26.63559102573157
42	26.237728653153468	22.23320855992655	25.427878616663136	26.101184170256847
43	25.58325682133861	21.91538950490854	25.71744708901288	26.783906584739974
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	5.0
5	5.0
6	4.5
7	4.0
8	3.0
9	2.0
10	4.5
11	7.0
12	7.0
13	13.0
14	19.0
15	33.5
16	48.0
17	39.0
18	30.0
19	30.0
20	46.0
21	62.0
22	70.5
23	79.0
24	107.0
25	135.0
26	135.0
27	180.5
28	226.0
29	295.0
30	364.0
31	460.5
32	557.0
33	557.0
34	690.0
35	823.0
36	1015.0
37	1207.0
38	1422.5
39	1638.0
40	1638.0
41	1791.5
42	1945.0
43	2052.5
44	2160.0
45	2473.0
46	2786.0
47	2786.0
48	2971.0
49	3156.0
50	3357.0
51	3558.0
52	3877.0
53	4196.0
54	4196.0
55	3945.0
56	3694.0
57	3569.0
58	3444.0
59	3235.0
60	3026.0
61	3026.0
62	2841.5
63	2657.0
64	2404.0
65	2151.0
66	1865.0
67	1579.0
68	1579.0
69	1341.0
70	1103.0
71	934.5
72	766.0
73	615.5
74	465.0
75	465.0
76	353.5
77	242.0
78	204.5
79	167.0
80	127.0
81	87.0
82	87.0
83	66.0
84	45.0
85	34.0
86	23.0
87	19.0
88	15.0
89	15.0
90	10.0
91	5.0
92	2.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	42477.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.88141817924995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.2824543020142	73.83054358829484
2	4.587262777971825	7.420486380864939
3	1.504831761555478	3.651387809873578
4	0.8673885202002561	2.8062245450479084
5	0.4540691582256374	1.8362878734373898
6	0.3201769705437187	1.5537820467547143
7	0.27069507509605306	1.5325941097535136
8	0.14844568634299685	0.9605198107210962
9	0.10769589009197811	0.7839536690444241
>10	0.4569798579578531	5.624220166207595
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAG	47	0.1106481154507145	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	44	0.10358546978364762	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0023542152223556276	0.0
24	0.0	0.0	0.0	0.004708430444711255	0.0
25	0.0	0.0	0.0	0.004708430444711255	0.0
26	0.0	0.0	0.0	0.00941686088942251	0.0
27	0.0	0.0	0.0	0.011771076111778139	0.0
28	0.0	0.0	0.0	0.035313228335334415	0.0
29	0.0	0.0	0.0	0.06356381100360195	0.0
30	0.0	0.0	0.0	0.10123125456129199	0.0
31	0.0	0.0	0.0	0.21423358523436212	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTTCC	20	0.0018220422	37.0	31
GTTTCCC	25	0.005437655	29.6	32
CCACTCC	25	0.005437655	29.6	33
CACTCCC	25	0.005437655	29.6	34
GTCAGCC	25	0.005437655	29.6	32
GGCAGCA	45	0.0037663416	20.555555	12
>>END_MODULE