FastQCFastQC Report
Fri 10 Feb 2017
ERR1630412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167901
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA20930.17921039540166503No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20170.17270299451751475No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC15430.13211736268741958No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC14950.1280074252869036No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT14500.12415435897391988No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG13930.11927380831080717No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA12680.10857084633029684No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG12120.1037759193630282No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC12110.10369029566718413No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT11820.10120720848770572No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC11780.1008647137043294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGC358.869483E-426.428578
GGTATCA6550.021.1832051
ACGGAGT909.478572E-820.55555526
TTAGGTT555.1431614E-420.1818184
ATCGACG555.1431614E-420.1818185
ATAATAC656.902253E-519.9230773
CAAGACG3600.019.013894
TCTAAAC609.236937E-418.53
GCTATAC500.007034949518.4999983
GTTACAC500.007034949518.4999983
ACTGATC1351.1514203E-917.8148148
AAGACGG3950.017.3291135
CTGGTCG1201.0412077E-716.9583349
CGATTTT803.3826227E-416.187521
CTAGACA1504.669346E-916.0333334
TCGACGG700.002592527815.8571426
GTATCAA8900.015.5898872
TATACTC957.059828E-515.5789485
ATACTAT1101.4518286E-515.1363646
GACGGAC4300.015.0581397
Produced by FastQC (version 0.11.5)