##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630412.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1167901
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.628143995081775	31.0	31.0	34.0	30.0	34.0
2	31.83633458657883	31.0	31.0	34.0	30.0	34.0
3	31.978014403618115	33.0	31.0	34.0	30.0	34.0
4	35.6579187790746	37.0	35.0	37.0	33.0	37.0
5	35.56577569502895	37.0	35.0	37.0	33.0	37.0
6	35.625017017709546	37.0	35.0	37.0	33.0	37.0
7	35.59508725482725	37.0	35.0	37.0	33.0	37.0
8	35.57043961774157	37.0	35.0	37.0	33.0	37.0
9	37.25877450229086	39.0	37.0	39.0	34.0	39.0
10	37.14758956452645	39.0	37.0	39.0	33.0	39.0
11	37.23112232971801	39.0	37.0	39.0	34.0	39.0
12	37.08907861197139	39.0	37.0	39.0	33.0	39.0
13	37.13070285923207	39.0	37.0	39.0	33.0	39.0
14	38.29433059822708	40.0	38.0	41.0	34.0	41.0
15	38.27481010804854	40.0	38.0	41.0	33.0	41.0
16	38.24901853838639	40.0	38.0	41.0	33.0	41.0
17	38.19171316746882	40.0	38.0	41.0	33.0	41.0
18	38.15808017974126	40.0	38.0	41.0	33.0	41.0
19	38.147530484176315	40.0	38.0	41.0	33.0	41.0
20	38.0984073136336	40.0	37.0	41.0	33.0	41.0
21	38.03647483819262	40.0	37.0	41.0	33.0	41.0
22	37.997529756374895	40.0	37.0	41.0	33.0	41.0
23	37.91249857650606	40.0	37.0	41.0	33.0	41.0
24	37.883861731431004	40.0	37.0	41.0	33.0	41.0
25	37.849808331356854	40.0	37.0	41.0	33.0	41.0
26	37.66771926730091	40.0	37.0	41.0	32.0	41.0
27	37.54779900008648	39.0	37.0	41.0	32.0	41.0
28	37.41333726060685	39.0	37.0	41.0	32.0	41.0
29	37.33836429628881	39.0	37.0	41.0	31.0	41.0
30	37.265498531125495	39.0	36.0	41.0	31.0	41.0
31	37.17205567937693	39.0	36.0	41.0	31.0	41.0
32	37.07187167405456	39.0	36.0	41.0	31.0	41.0
33	36.96791851364114	39.0	36.0	40.0	30.0	41.0
34	36.87364511204289	39.0	36.0	40.0	30.0	41.0
35	36.76278040690093	39.0	35.0	40.0	30.0	41.0
36	36.65535263691015	39.0	35.0	40.0	30.0	41.0
37	36.58799761281136	39.0	35.0	40.0	30.0	41.0
38	36.458322237929416	39.0	35.0	40.0	30.0	41.0
39	36.35092101128435	39.0	35.0	40.0	30.0	41.0
40	36.215561079235314	38.0	35.0	40.0	29.0	41.0
41	36.0794219715541	38.0	35.0	40.0	29.0	41.0
42	35.993714364488085	38.0	35.0	40.0	29.0	41.0
43	34.67679366658646	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	2.0
10	4.0
11	1.0
12	2.0
13	2.0
14	6.0
15	5.0
16	16.0
17	32.0
18	82.0
19	137.0
20	286.0
21	591.0
22	1059.0
23	1876.0
24	2850.0
25	4275.0
26	6242.0
27	9147.0
28	12413.0
29	17190.0
30	22185.0
31	29005.0
32	36864.0
33	47551.0
34	62436.0
35	82139.0
36	113901.0
37	169303.0
38	290908.0
39	257389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.965114337602245	18.54343818525714	11.720085863442192	26.771361613698417
2	20.896634218140065	20.154448022563557	31.32654223260362	27.62237552669276
3	20.94244289541665	21.47330980964996	28.310704417583338	29.27354287735005
4	15.556712426823848	15.033209150433127	32.60755834612694	36.802520076616084
5	16.43683839640517	34.736762790681745	32.60216405328876	16.224234759624316
6	37.2914313798858	33.22499081685862	14.609200608613229	14.874377194642355
7	31.276281123143146	28.911183396537893	19.87394479497834	19.938590685340625
8	28.944405390525397	31.127210268678596	18.82128707827119	21.10709726252482
9	27.330912466039504	13.418174999422039	18.329635816734466	40.92127671780399
10	18.52836841478858	24.612873865164943	31.03516479564621	25.82359292440027
11	39.298536434166934	20.075417351299468	19.547461642724855	21.07858457180874
12	23.215580772685357	23.88173312635232	27.0531491967213	25.849536904241027
13	31.609785418455843	17.7112614853485	23.470140020429813	27.208813075765843
14	24.051696162602823	19.377327359082663	22.54480473944281	34.0261717388717
15	27.157181987171857	26.213780106361757	19.95066362645464	26.678374280011745
16	27.801585922094425	24.132096813000416	21.686255941214196	26.380061323690963
17	26.228593005742777	24.48452394509466	22.75783649470289	26.52904655445967
18	26.535896450127193	22.587787834756544	24.152988994786373	26.723326720329894
19	26.531272770551613	23.52904912317054	23.269523701067126	26.670154405210717
20	26.65200218169177	22.780783645189103	23.395476157653775	27.17173801546535
21	27.88669587576344	23.062057486036917	23.190749900890573	25.86049673730907
22	27.543087984341135	22.995271003278532	23.154787948636056	26.306853063744274
23	26.210183911136305	22.791914725648834	23.771364182409297	27.226537180805565
24	26.20050843350592	23.743622104955815	23.34487255340992	26.710996908128344
25	26.99475383615563	23.063170594082887	23.153417969502552	26.788657600258926
26	27.075068862857382	23.95656823652005	23.182872520872916	25.785490379749653
27	27.532556269752316	22.730608159424474	23.080552204339238	26.656283366483972
28	26.188777987175282	23.15821289646982	24.080037605927217	26.572971510427685
29	25.63941635463965	23.344530058626546	24.101101035104858	26.914952551628947
30	25.29777780822176	24.26781037091329	24.20744566534321	26.22696615552174
31	27.02403714013431	23.731634787537644	22.848768859689304	26.39555921263874
32	26.14408241794467	23.265071268883236	22.902711788071077	27.688134525101017
33	25.55156644270362	23.073787932367555	23.99835260009196	27.37629302483687
34	27.14236908779083	22.91255851309315	23.414142123347784	26.530930275768238
35	26.83652124623577	22.922490861811063	24.349067258269322	25.891920633683846
36	26.017273724399587	23.77710097003085	23.731634787537644	26.47399051803192
37	26.747729473645453	22.85364941035242	24.08937058877422	26.309250527227906
38	25.49205797409198	22.70868849328839	24.95990670442101	26.839346828198625
39	26.280138470640917	22.22619896720698	24.591125446420545	26.902537115731555
40	25.71151150654037	22.701239231749952	25.17867524730264	26.408574014407044
41	24.977031443589826	22.78592106693975	26.012221926344786	26.22482556312564
42	25.97840056648637	22.502249762608304	25.954083436866654	25.565266234038674
43	24.821966930416192	21.640361640241768	26.034055968785026	27.50361546055702
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	30.0
2	43.0
3	75.5
4	108.0
5	108.0
6	148.5
7	189.0
8	188.0
9	187.0
10	242.0
11	297.0
12	297.0
13	596.0
14	895.0
15	1348.5
16	1802.0
17	1902.0
18	2002.0
19	2002.0
20	2414.0
21	2826.0
22	2994.5
23	3163.0
24	4013.0
25	4863.0
26	4863.0
27	6314.0
28	7765.0
29	9613.5
30	11462.0
31	13884.5
32	16307.0
33	16307.0
34	20461.0
35	24615.0
36	29698.5
37	34782.0
38	40996.5
39	47211.0
40	47211.0
41	51606.5
42	56002.0
43	60192.0
44	64382.0
45	69680.5
46	74979.0
47	74979.0
48	81142.0
49	87305.0
50	89305.5
51	91306.0
52	95279.0
53	99252.0
54	99252.0
55	96408.5
56	93565.0
57	90768.5
58	87972.0
59	86398.5
60	84825.0
61	84825.0
62	80082.5
63	75340.0
64	68824.5
65	62309.0
66	55169.5
67	48030.0
68	48030.0
69	40239.5
70	32449.0
71	27820.0
72	23191.0
73	17595.5
74	12000.0
75	12000.0
76	9212.0
77	6424.0
78	5143.0
79	3862.0
80	3337.0
81	2812.0
82	2812.0
83	2006.5
84	1201.0
85	1040.0
86	879.0
87	768.0
88	657.0
89	657.0
90	589.5
91	522.0
92	293.5
93	65.0
94	42.0
95	19.0
96	19.0
97	17.0
98	15.0
99	9.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1167901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.805377477196764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.10045616670428	40.89044210432753
2	9.692855410838847	9.655126451374356
3	3.025079562826683	4.519956885754088
4	1.4071172121257896	2.8032801561834275
5	0.8126713933068715	2.023770275428409
6	0.5315457783407992	1.5884302882004315
7	0.3797615616608439	1.3239917550893643
8	0.2717196644379185	1.0826480364246212
9	0.20717188349459506	0.9286446474099123
>10	1.3031524516733946	12.421401180318584
>50	0.13563110793368938	4.78391718120547
>100	0.11778977472915253	11.920754703221471
>500	0.011588714242766576	3.864006657938044
>1k	0.003459317684407933	2.1936296771243224
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2093	0.17921039540166503	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2017	0.17270299451751475	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1543	0.13211736268741958	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1495	0.1280074252869036	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1450	0.12415435897391988	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	1393	0.11927380831080717	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	1268	0.10857084633029684	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	1212	0.1037759193630282	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1211	0.10369029566718413	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1182	0.10120720848770572	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1178	0.1008647137043294	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.562369584408268E-5	0.0
5	0.0	0.0	0.0	8.562369584408268E-5	0.0
6	0.0	0.0	0.0	8.562369584408268E-5	0.0
7	0.0	0.0	0.0	8.562369584408268E-5	0.0
8	0.0	0.0	0.0	8.562369584408268E-5	0.0
9	0.0	0.0	0.0	8.562369584408268E-5	0.0
10	0.0	0.0	0.0	8.562369584408268E-5	0.0
11	0.0	0.0	0.0	8.562369584408268E-5	0.0
12	0.0	0.0	0.0	8.562369584408268E-5	0.0
13	0.0	0.0	0.0	8.562369584408268E-5	0.0
14	0.0	0.0	0.0	8.562369584408268E-5	0.0
15	0.0	0.0	0.0	1.7124739168816535E-4	0.0
16	0.0	0.0	0.0	5.993658709085787E-4	0.0
17	0.0	0.0	0.0	9.418606542849094E-4	0.0
18	0.0	0.0	0.0	0.0011131080459730748	0.0
19	0.0	0.0	0.0	0.0014556028293494054	0.0
20	0.0	0.0	0.0	0.0018837213085698189	0.0
21	0.0	0.0	0.0	0.0023974634836343147	0.0
22	0.0	0.0	0.0	0.0037674426171396378	0.0
23	0.0	0.0	0.0	0.005394292838177208	0.0
24	0.0	0.0	0.0	0.007449261538435193	0.0
25	0.0	0.0	0.0	0.009332982847005011	0.0
26	0.0	0.0	0.0	0.012586683289080153	0.0
27	0.0	0.0	0.0	0.02465962440309581	0.0
28	0.0	0.0	0.0	0.05120297011476144	0.0
29	0.0	0.0	0.0	0.097354142174722	0.0
30	0.0	0.0	0.0	0.16020193492427867	0.0
31	0.0	0.0	0.0	0.31089963960986416	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGC	35	8.869483E-4	26.42857	8
GGTATCA	655	0.0	21.183205	1
ACGGAGT	90	9.478572E-8	20.555555	26
TTAGGTT	55	5.1431614E-4	20.181818	4
ATCGACG	55	5.1431614E-4	20.181818	5
ATAATAC	65	6.902253E-5	19.923077	3
CAAGACG	360	0.0	19.01389	4
TCTAAAC	60	9.236937E-4	18.5	3
GCTATAC	50	0.0070349495	18.499998	3
GTTACAC	50	0.0070349495	18.499998	3
ACTGATC	135	1.1514203E-9	17.814814	8
AAGACGG	395	0.0	17.329113	5
CTGGTCG	120	1.0412077E-7	16.958334	9
CGATTTT	80	3.3826227E-4	16.1875	21
CTAGACA	150	4.669346E-9	16.033333	4
TCGACGG	70	0.0025925278	15.857142	6
GTATCAA	890	0.0	15.589887	2
TATACTC	95	7.059828E-5	15.578948	5
ATACTAT	110	1.4518286E-5	15.136364	6
GACGGAC	430	0.0	15.058139	7
>>END_MODULE